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Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel

Localized intra-periodontal pocket drug delivery using an injectable in situ forming gel is an effective periodontitis treatment. The aqueous insoluble property of rosin is suitable for preparing a solvent exchange-induced in situ forming gel. This study aims to investigate the role of incorporating...

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Autores principales: Khaing, Ei Mon, Mahadlek, Jongjan, Okonogi, Siriporn, Phaechamud, Thawatchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951584/
https://www.ncbi.nlm.nih.gov/pubmed/35323282
http://dx.doi.org/10.3390/gels8030169
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author Khaing, Ei Mon
Mahadlek, Jongjan
Okonogi, Siriporn
Phaechamud, Thawatchai
author_facet Khaing, Ei Mon
Mahadlek, Jongjan
Okonogi, Siriporn
Phaechamud, Thawatchai
author_sort Khaing, Ei Mon
collection PubMed
description Localized intra-periodontal pocket drug delivery using an injectable in situ forming gel is an effective periodontitis treatment. The aqueous insoluble property of rosin is suitable for preparing a solvent exchange-induced in situ forming gel. This study aims to investigate the role of incorporating lime peel oil (LO) on the physicochemical properties of injectable in situ forming gels based on rosin loaded with 5% w/w doxycycline hyclate (DH) in dimethyl sulfoxide (DMSO) and N-methyl pyrrolidone (NMP). Their gel formation, viscosity, injectability, mechanical properties, wettability, drug release, and antimicrobial activities were evaluated. The presence of LO slowed gel formation due to the loose precipitate formation of gel with a high LO content. The viscosity and injectability were slightly increased with higher LO content for the DH-loaded rosin-based in situ forming gel. The addition of 10% LO lowered gel hardness with higher adhesion. LO incorporation promoted a higher drug release pattern than the no oil-added formulation over 10 days and the gel formation rate related to burst drug release. The drug release kinetics followed the non-Fickian diffusion mechanism for oil-added formulations. LO exhibited high antimicrobial activity against Porphyromonas gingivalis and Staphylococcus aureus. The DH-loaded rosin in situ forming gel with an addition of LO (0, 2.5, 5, and 10% w/w) inhibited all tested microorganisms. Adding 10% LO to rosin-based in situ forming gel improved the antimicrobial activities, especially for the P. gingivalis and S. aureus. As a result, the study demonstrates the possibility of using an LO amount of less than 10% loading into a rosin-based in situ forming gel for efficient periodontitis treatment.
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spelling pubmed-89515842022-03-26 Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel Khaing, Ei Mon Mahadlek, Jongjan Okonogi, Siriporn Phaechamud, Thawatchai Gels Article Localized intra-periodontal pocket drug delivery using an injectable in situ forming gel is an effective periodontitis treatment. The aqueous insoluble property of rosin is suitable for preparing a solvent exchange-induced in situ forming gel. This study aims to investigate the role of incorporating lime peel oil (LO) on the physicochemical properties of injectable in situ forming gels based on rosin loaded with 5% w/w doxycycline hyclate (DH) in dimethyl sulfoxide (DMSO) and N-methyl pyrrolidone (NMP). Their gel formation, viscosity, injectability, mechanical properties, wettability, drug release, and antimicrobial activities were evaluated. The presence of LO slowed gel formation due to the loose precipitate formation of gel with a high LO content. The viscosity and injectability were slightly increased with higher LO content for the DH-loaded rosin-based in situ forming gel. The addition of 10% LO lowered gel hardness with higher adhesion. LO incorporation promoted a higher drug release pattern than the no oil-added formulation over 10 days and the gel formation rate related to burst drug release. The drug release kinetics followed the non-Fickian diffusion mechanism for oil-added formulations. LO exhibited high antimicrobial activity against Porphyromonas gingivalis and Staphylococcus aureus. The DH-loaded rosin in situ forming gel with an addition of LO (0, 2.5, 5, and 10% w/w) inhibited all tested microorganisms. Adding 10% LO to rosin-based in situ forming gel improved the antimicrobial activities, especially for the P. gingivalis and S. aureus. As a result, the study demonstrates the possibility of using an LO amount of less than 10% loading into a rosin-based in situ forming gel for efficient periodontitis treatment. MDPI 2022-03-08 /pmc/articles/PMC8951584/ /pubmed/35323282 http://dx.doi.org/10.3390/gels8030169 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khaing, Ei Mon
Mahadlek, Jongjan
Okonogi, Siriporn
Phaechamud, Thawatchai
Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel
title Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel
title_full Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel
title_fullStr Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel
title_full_unstemmed Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel
title_short Lime Peel Oil–Incorporated Rosin-Based Antimicrobial In Situ Forming Gel
title_sort lime peel oil–incorporated rosin-based antimicrobial in situ forming gel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951584/
https://www.ncbi.nlm.nih.gov/pubmed/35323282
http://dx.doi.org/10.3390/gels8030169
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