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Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering

Background: Meniscus tissue engineering has yet to achieve clinical application because it requires chondrogenic induction and in vitro cell expansion. Contrarily, cartilage engineering from autologous chondrocytes has been successfully applied in one-stage surgery. If the natural chondrogenic poten...

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Autores principales: Numpaisal, Piya-on, Jiang, Ching-Chuan, Hsieh, Chang-Hsun, Chiang, Hongsen, Chien, Chung-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952194/
https://www.ncbi.nlm.nih.gov/pubmed/35335980
http://dx.doi.org/10.3390/pharmaceutics14030605
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author Numpaisal, Piya-on
Jiang, Ching-Chuan
Hsieh, Chang-Hsun
Chiang, Hongsen
Chien, Chung-Liang
author_facet Numpaisal, Piya-on
Jiang, Ching-Chuan
Hsieh, Chang-Hsun
Chiang, Hongsen
Chien, Chung-Liang
author_sort Numpaisal, Piya-on
collection PubMed
description Background: Meniscus tissue engineering has yet to achieve clinical application because it requires chondrogenic induction and in vitro cell expansion. Contrarily, cartilage engineering from autologous chondrocytes has been successfully applied in one-stage surgery. If the natural chondrogenic potential of meniscus cells can be demonstrated, meniscus tissue engineering would have more value in clinical settings. Materials and Methods: In total, 10 menisci and pieces of cartilage were obtained during total knee replacements. The tissues were collected for cell isolation and expansion. Their chondrogenic properties were examined by immunohistofluorescence and gene expression analyses. Results: In native cartilage, immunofluorescence demonstrated the presence of collagen I, aggrecan, and traces of collagen I, whereas comparable staining was seen in the inner and middle meniscus. The presence of collagen I but the absence of collagen II and aggrecan were observed in the outer meniscus. In passage 2, chondrocytes showed the presence of collagen II and aggrecan, and the absence of vimentin. The vimentin and aggrecan staining were comparable in the inner and middle meniscus cells, whereas the outer cells showed only vimentin staining. In the gene expression analyses, the expressions of collagen II and aggrecan in the native chondrocyte and the inner and middle meniscus were higher than those of the cells from the outer meniscus, but they were not different in collagen I. In the passage 2 culture, chondrocytes had a higher expression of collagen II and aggrecan than the meniscus cells. Cells from the inner and middle areas had higher collagen II and aggrecan expression than those from the outer meniscus. Conclusion: Without chondrogenic induction, inner and middle meniscus cells possess a chondrogenic phenotype. Specifically, native meniscus cells exhibited more robust chondrogenic potential compared with those of the passage 2 monolayer culture.
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spelling pubmed-89521942022-03-26 Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering Numpaisal, Piya-on Jiang, Ching-Chuan Hsieh, Chang-Hsun Chiang, Hongsen Chien, Chung-Liang Pharmaceutics Article Background: Meniscus tissue engineering has yet to achieve clinical application because it requires chondrogenic induction and in vitro cell expansion. Contrarily, cartilage engineering from autologous chondrocytes has been successfully applied in one-stage surgery. If the natural chondrogenic potential of meniscus cells can be demonstrated, meniscus tissue engineering would have more value in clinical settings. Materials and Methods: In total, 10 menisci and pieces of cartilage were obtained during total knee replacements. The tissues were collected for cell isolation and expansion. Their chondrogenic properties were examined by immunohistofluorescence and gene expression analyses. Results: In native cartilage, immunofluorescence demonstrated the presence of collagen I, aggrecan, and traces of collagen I, whereas comparable staining was seen in the inner and middle meniscus. The presence of collagen I but the absence of collagen II and aggrecan were observed in the outer meniscus. In passage 2, chondrocytes showed the presence of collagen II and aggrecan, and the absence of vimentin. The vimentin and aggrecan staining were comparable in the inner and middle meniscus cells, whereas the outer cells showed only vimentin staining. In the gene expression analyses, the expressions of collagen II and aggrecan in the native chondrocyte and the inner and middle meniscus were higher than those of the cells from the outer meniscus, but they were not different in collagen I. In the passage 2 culture, chondrocytes had a higher expression of collagen II and aggrecan than the meniscus cells. Cells from the inner and middle areas had higher collagen II and aggrecan expression than those from the outer meniscus. Conclusion: Without chondrogenic induction, inner and middle meniscus cells possess a chondrogenic phenotype. Specifically, native meniscus cells exhibited more robust chondrogenic potential compared with those of the passage 2 monolayer culture. MDPI 2022-03-10 /pmc/articles/PMC8952194/ /pubmed/35335980 http://dx.doi.org/10.3390/pharmaceutics14030605 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Numpaisal, Piya-on
Jiang, Ching-Chuan
Hsieh, Chang-Hsun
Chiang, Hongsen
Chien, Chung-Liang
Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering
title Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering
title_full Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering
title_fullStr Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering
title_full_unstemmed Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering
title_short Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering
title_sort prospective application of partially digested autologous chondrocyte for meniscus tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952194/
https://www.ncbi.nlm.nih.gov/pubmed/35335980
http://dx.doi.org/10.3390/pharmaceutics14030605
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