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Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats

Caloric restriction (CR) has been shown to be an effective nutritional intervention for increasing longevity in some animal species. The objective of this study was to evaluate CR’s effects on metabolic and reproductive parameters in 12-month-old male Wistar rats. The rats were distributed in three...

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Autores principales: de Jesús, Pablo López, Arenas-Ríos, Edith, Ruíz-Ramos, Mirna, Flores-Alonso, Juan Carlos, Mendoza-Núñez, Víctor Manuel, Arrieta-Cruz, Isabel, Arteaga-Silva, Marcela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952234/
https://www.ncbi.nlm.nih.gov/pubmed/35334913
http://dx.doi.org/10.3390/nu14061256
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author de Jesús, Pablo López
Arenas-Ríos, Edith
Ruíz-Ramos, Mirna
Flores-Alonso, Juan Carlos
Mendoza-Núñez, Víctor Manuel
Arrieta-Cruz, Isabel
Arteaga-Silva, Marcela
author_facet de Jesús, Pablo López
Arenas-Ríos, Edith
Ruíz-Ramos, Mirna
Flores-Alonso, Juan Carlos
Mendoza-Núñez, Víctor Manuel
Arrieta-Cruz, Isabel
Arteaga-Silva, Marcela
author_sort de Jesús, Pablo López
collection PubMed
description Caloric restriction (CR) has been shown to be an effective nutritional intervention for increasing longevity in some animal species. The objective of this study was to evaluate CR’s effects on metabolic and reproductive parameters in 12-month-old male Wistar rats. The rats were distributed in three groups: control, CR at 15%, and CR at 35% for 6 (up to 18 months of age) and 12 months (up to 24 months of age). At the end of CR treatment, we evaluated reproductive (male sexual behavior (MSB), sperm quality) and biochemical parameters (plasma glucose, glucose-regulating hormone, and sex steroid levels), and quantified annexin V in the seminiferous epithelium. Results showed that MSB and sperm quality were improved after 6 months of CR associated with increases in plasma testosterone and decrease annexin V in the seminiferous epithelium of the testicles compared to their control group. The metabolic profile of the CR rats also improved compared to controls. However, these effects of CR on reproductive parameters were not maintained after 12 months of CR. Findings suggest that beginning CR at the age of maturity reestablishes the behavioral sexual response and reproductive function in older animals after 6 months of CR and improves endocrine functioning during aging.
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spelling pubmed-89522342022-03-26 Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats de Jesús, Pablo López Arenas-Ríos, Edith Ruíz-Ramos, Mirna Flores-Alonso, Juan Carlos Mendoza-Núñez, Víctor Manuel Arrieta-Cruz, Isabel Arteaga-Silva, Marcela Nutrients Article Caloric restriction (CR) has been shown to be an effective nutritional intervention for increasing longevity in some animal species. The objective of this study was to evaluate CR’s effects on metabolic and reproductive parameters in 12-month-old male Wistar rats. The rats were distributed in three groups: control, CR at 15%, and CR at 35% for 6 (up to 18 months of age) and 12 months (up to 24 months of age). At the end of CR treatment, we evaluated reproductive (male sexual behavior (MSB), sperm quality) and biochemical parameters (plasma glucose, glucose-regulating hormone, and sex steroid levels), and quantified annexin V in the seminiferous epithelium. Results showed that MSB and sperm quality were improved after 6 months of CR associated with increases in plasma testosterone and decrease annexin V in the seminiferous epithelium of the testicles compared to their control group. The metabolic profile of the CR rats also improved compared to controls. However, these effects of CR on reproductive parameters were not maintained after 12 months of CR. Findings suggest that beginning CR at the age of maturity reestablishes the behavioral sexual response and reproductive function in older animals after 6 months of CR and improves endocrine functioning during aging. MDPI 2022-03-16 /pmc/articles/PMC8952234/ /pubmed/35334913 http://dx.doi.org/10.3390/nu14061256 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Jesús, Pablo López
Arenas-Ríos, Edith
Ruíz-Ramos, Mirna
Flores-Alonso, Juan Carlos
Mendoza-Núñez, Víctor Manuel
Arrieta-Cruz, Isabel
Arteaga-Silva, Marcela
Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats
title Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats
title_full Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats
title_fullStr Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats
title_full_unstemmed Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats
title_short Effect of Chronic Moderate Caloric Restriction on the Reproductive Function in Aged Male Wistar Rats
title_sort effect of chronic moderate caloric restriction on the reproductive function in aged male wistar rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952234/
https://www.ncbi.nlm.nih.gov/pubmed/35334913
http://dx.doi.org/10.3390/nu14061256
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