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The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis

Monocytes expressing the inflammation suppressing active CD11b, a beta2 integrin, may regulate neuroinflammation and modify clinical outcomes in amyotrophic lateral sclerosis (ALS). In this single site, retrospective study, peripheral blood mononuclear cells from 38 individuals living with ALS and 2...

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Autores principales: Yildiz, Ozlem, Schroth, Johannes, Lombardi, Vittoria, Pucino, Valentina, Bobeva, Yoana, Yip, Ping Kei, Schmierer, Klaus, Mauro, Claudio, Tree, Timothy, Henson, Sian Mari, Malaspina, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952310/
https://www.ncbi.nlm.nih.gov/pubmed/35328793
http://dx.doi.org/10.3390/ijms23063370
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author Yildiz, Ozlem
Schroth, Johannes
Lombardi, Vittoria
Pucino, Valentina
Bobeva, Yoana
Yip, Ping Kei
Schmierer, Klaus
Mauro, Claudio
Tree, Timothy
Henson, Sian Mari
Malaspina, Andrea
author_facet Yildiz, Ozlem
Schroth, Johannes
Lombardi, Vittoria
Pucino, Valentina
Bobeva, Yoana
Yip, Ping Kei
Schmierer, Klaus
Mauro, Claudio
Tree, Timothy
Henson, Sian Mari
Malaspina, Andrea
author_sort Yildiz, Ozlem
collection PubMed
description Monocytes expressing the inflammation suppressing active CD11b, a beta2 integrin, may regulate neuroinflammation and modify clinical outcomes in amyotrophic lateral sclerosis (ALS). In this single site, retrospective study, peripheral blood mononuclear cells from 38 individuals living with ALS and 20 non-neurological controls (NNC) were investigated using flow cytometry to study active CD11b integrin classical (CM), intermediate (IM) and non-classical (NCM) monocytes during ALS progression. Seventeen ALS participants were sampled at the baseline (V1) and at two additional time points (V2 and V3) for longitudinal analysis. Active CD11b+ CM frequencies increased steeply between the baseline and V3 (ANOVA repeated measurement, p < 0.001), and the V2/V1 ratio negatively correlated with the disease progression rate, similar to higher frequencies of active CD11b+ NCM at the baseline (R = −0.6567; p = 0.0031 and R = 0.3862; p = 0.0168, respectively). CD11b NCM, clinical covariates and neurofilament light-chain plasma concentration at the baseline predicted shorter survival in a multivariable and univariate analysis (CD11b NCM—HR: 1.05, CI: 1.01–1.11, p = 0.013. Log rank: above median: 43 months and below median: 21.22 months; p = 0.0022). Blood samples with the highest frequencies of active CD11b+ IM and NCM contained the lowest concentrations of soluble CD11b. Our preliminary data suggest that the levels of active CD11b+ monocytes and NCM in the blood predict different clinical outcomes in ALS.
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spelling pubmed-89523102022-03-26 The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis Yildiz, Ozlem Schroth, Johannes Lombardi, Vittoria Pucino, Valentina Bobeva, Yoana Yip, Ping Kei Schmierer, Klaus Mauro, Claudio Tree, Timothy Henson, Sian Mari Malaspina, Andrea Int J Mol Sci Article Monocytes expressing the inflammation suppressing active CD11b, a beta2 integrin, may regulate neuroinflammation and modify clinical outcomes in amyotrophic lateral sclerosis (ALS). In this single site, retrospective study, peripheral blood mononuclear cells from 38 individuals living with ALS and 20 non-neurological controls (NNC) were investigated using flow cytometry to study active CD11b integrin classical (CM), intermediate (IM) and non-classical (NCM) monocytes during ALS progression. Seventeen ALS participants were sampled at the baseline (V1) and at two additional time points (V2 and V3) for longitudinal analysis. Active CD11b+ CM frequencies increased steeply between the baseline and V3 (ANOVA repeated measurement, p < 0.001), and the V2/V1 ratio negatively correlated with the disease progression rate, similar to higher frequencies of active CD11b+ NCM at the baseline (R = −0.6567; p = 0.0031 and R = 0.3862; p = 0.0168, respectively). CD11b NCM, clinical covariates and neurofilament light-chain plasma concentration at the baseline predicted shorter survival in a multivariable and univariate analysis (CD11b NCM—HR: 1.05, CI: 1.01–1.11, p = 0.013. Log rank: above median: 43 months and below median: 21.22 months; p = 0.0022). Blood samples with the highest frequencies of active CD11b+ IM and NCM contained the lowest concentrations of soluble CD11b. Our preliminary data suggest that the levels of active CD11b+ monocytes and NCM in the blood predict different clinical outcomes in ALS. MDPI 2022-03-21 /pmc/articles/PMC8952310/ /pubmed/35328793 http://dx.doi.org/10.3390/ijms23063370 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yildiz, Ozlem
Schroth, Johannes
Lombardi, Vittoria
Pucino, Valentina
Bobeva, Yoana
Yip, Ping Kei
Schmierer, Klaus
Mauro, Claudio
Tree, Timothy
Henson, Sian Mari
Malaspina, Andrea
The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis
title The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis
title_full The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis
title_fullStr The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis
title_full_unstemmed The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis
title_short The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis
title_sort expression of active cd11b monocytes in blood and disease progression in amyotrophic lateral sclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952310/
https://www.ncbi.nlm.nih.gov/pubmed/35328793
http://dx.doi.org/10.3390/ijms23063370
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