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In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes
Microvascular disease is frequently found in major pathologies affecting vital organs, such as the brain, heart, and kidneys. While imaging modalities, such as ultrasound, computed tomography, single photon emission computed tomography, and magnetic resonance imaging, are widely used to visualize va...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953049/ https://www.ncbi.nlm.nih.gov/pubmed/35337090 http://dx.doi.org/10.3390/ph15030292 |
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author | Wang, Shaowei Budzevich, Mikalai Abdalah, Mahmoud A. Balagurunathan, Yoganand Choi, Jung W. |
author_facet | Wang, Shaowei Budzevich, Mikalai Abdalah, Mahmoud A. Balagurunathan, Yoganand Choi, Jung W. |
author_sort | Wang, Shaowei |
collection | PubMed |
description | Microvascular disease is frequently found in major pathologies affecting vital organs, such as the brain, heart, and kidneys. While imaging modalities, such as ultrasound, computed tomography, single photon emission computed tomography, and magnetic resonance imaging, are widely used to visualize vascular abnormalities, the ability to non-invasively assess an organ’s total vasculature, including microvasculature, is often limited or cumbersome. Previously, we have demonstrated proof of concept that non-invasive imaging of the total mouse vasculature can be achieved with 18F-fluorodeoxyglucose (18F-FDG)-labeled human erythrocytes and positron emission tomography/computerized tomography (PET/CT). In this work, we demonstrate that changes in the total vascular volume of the brain and left ventricular myocardium of normal rats can be seen after pharmacological vasodilation using 18F-FDG-labeled rat red blood cells (FDG RBCs) and microPET/CT imaging. FDG RBC PET imaging was also used to approximate the location of myocardial injury in a surgical myocardial infarction rat model. Finally, we show that FDG RBC PET imaging can detect relative differences in the degree of drug-induced intra-myocardial vasodilation between diabetic rats and normal controls. This FDG-labeled RBC PET imaging technique may thus be useful for assessing microvascular disease pathologies and characterizing pharmacological responses in the vascular bed of interest. |
format | Online Article Text |
id | pubmed-8953049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89530492022-03-26 In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes Wang, Shaowei Budzevich, Mikalai Abdalah, Mahmoud A. Balagurunathan, Yoganand Choi, Jung W. Pharmaceuticals (Basel) Article Microvascular disease is frequently found in major pathologies affecting vital organs, such as the brain, heart, and kidneys. While imaging modalities, such as ultrasound, computed tomography, single photon emission computed tomography, and magnetic resonance imaging, are widely used to visualize vascular abnormalities, the ability to non-invasively assess an organ’s total vasculature, including microvasculature, is often limited or cumbersome. Previously, we have demonstrated proof of concept that non-invasive imaging of the total mouse vasculature can be achieved with 18F-fluorodeoxyglucose (18F-FDG)-labeled human erythrocytes and positron emission tomography/computerized tomography (PET/CT). In this work, we demonstrate that changes in the total vascular volume of the brain and left ventricular myocardium of normal rats can be seen after pharmacological vasodilation using 18F-FDG-labeled rat red blood cells (FDG RBCs) and microPET/CT imaging. FDG RBC PET imaging was also used to approximate the location of myocardial injury in a surgical myocardial infarction rat model. Finally, we show that FDG RBC PET imaging can detect relative differences in the degree of drug-induced intra-myocardial vasodilation between diabetic rats and normal controls. This FDG-labeled RBC PET imaging technique may thus be useful for assessing microvascular disease pathologies and characterizing pharmacological responses in the vascular bed of interest. MDPI 2022-02-27 /pmc/articles/PMC8953049/ /pubmed/35337090 http://dx.doi.org/10.3390/ph15030292 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Shaowei Budzevich, Mikalai Abdalah, Mahmoud A. Balagurunathan, Yoganand Choi, Jung W. In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes |
title | In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes |
title_full | In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes |
title_fullStr | In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes |
title_full_unstemmed | In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes |
title_short | In Vivo Imaging of Rat Vascularity with FDG-Labeled Erythrocytes |
title_sort | in vivo imaging of rat vascularity with fdg-labeled erythrocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953049/ https://www.ncbi.nlm.nih.gov/pubmed/35337090 http://dx.doi.org/10.3390/ph15030292 |
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