Cargando…

Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma

Few studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB...

Descripción completa

Detalles Bibliográficos
Autores principales: Trevissón, Bibiana, Becerro-de-Bengoa-Vallejo, Ricardo, Sevillano, David, González, Natalia, Losa-Iglesias, Marta Elena, López-López, Daniel, Alou, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953315/
https://www.ncbi.nlm.nih.gov/pubmed/35330008
http://dx.doi.org/10.3390/jcm11061683
_version_ 1784675819952537600
author Trevissón, Bibiana
Becerro-de-Bengoa-Vallejo, Ricardo
Sevillano, David
González, Natalia
Losa-Iglesias, Marta Elena
López-López, Daniel
Alou, Luis
author_facet Trevissón, Bibiana
Becerro-de-Bengoa-Vallejo, Ricardo
Sevillano, David
González, Natalia
Losa-Iglesias, Marta Elena
López-López, Daniel
Alou, Luis
author_sort Trevissón, Bibiana
collection PubMed
description Few studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB and PRP were compared between genders, and after the population was split into pre (≤50 years)- and post (>50 years)-menopausal ages. In WB, platelet–total WBC densities were comparable in men and women. The phagocytic cell composition differed (p ≤ 0.04). After dividing by ages, phagocytic cell discrepancies were linked to women > 50 years (p ≤ 0.05), and differences emerged in lymphocyte counts (lower in >50 years groups, within and between genders, p ≤ 0.05). In PRP, densities were significantly higher, but the PRP/WB ratios varied according to blood cell (lower for phagocytic cells) and between subjects (more favorable at a lower density of a particular blood cell in WB). This “system compensatory efficiency” reduced/reverted PRP differences in the leukocyte composition between genders/age–sex groups in WB. In PRP, neutrophils were higher in younger men than older women (p < 0.05). WB lymphocyte differences between age–sex groups persisted. Age is a more determining factor than sex in the preparation of PRP. Post-menopause, sexual dimorphism strongly influences the composition of leukocytes, also conditioned by the capture efficiency of the system.
format Online
Article
Text
id pubmed-8953315
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89533152022-03-26 Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma Trevissón, Bibiana Becerro-de-Bengoa-Vallejo, Ricardo Sevillano, David González, Natalia Losa-Iglesias, Marta Elena López-López, Daniel Alou, Luis J Clin Med Article Few studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB and PRP were compared between genders, and after the population was split into pre (≤50 years)- and post (>50 years)-menopausal ages. In WB, platelet–total WBC densities were comparable in men and women. The phagocytic cell composition differed (p ≤ 0.04). After dividing by ages, phagocytic cell discrepancies were linked to women > 50 years (p ≤ 0.05), and differences emerged in lymphocyte counts (lower in >50 years groups, within and between genders, p ≤ 0.05). In PRP, densities were significantly higher, but the PRP/WB ratios varied according to blood cell (lower for phagocytic cells) and between subjects (more favorable at a lower density of a particular blood cell in WB). This “system compensatory efficiency” reduced/reverted PRP differences in the leukocyte composition between genders/age–sex groups in WB. In PRP, neutrophils were higher in younger men than older women (p < 0.05). WB lymphocyte differences between age–sex groups persisted. Age is a more determining factor than sex in the preparation of PRP. Post-menopause, sexual dimorphism strongly influences the composition of leukocytes, also conditioned by the capture efficiency of the system. MDPI 2022-03-18 /pmc/articles/PMC8953315/ /pubmed/35330008 http://dx.doi.org/10.3390/jcm11061683 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trevissón, Bibiana
Becerro-de-Bengoa-Vallejo, Ricardo
Sevillano, David
González, Natalia
Losa-Iglesias, Marta Elena
López-López, Daniel
Alou, Luis
Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
title Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
title_full Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
title_fullStr Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
title_full_unstemmed Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
title_short Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
title_sort influence of sexual dimorphism, aging, and differential cell capture efficiency of blood separation systems on the quality of platelet-rich plasma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953315/
https://www.ncbi.nlm.nih.gov/pubmed/35330008
http://dx.doi.org/10.3390/jcm11061683
work_keys_str_mv AT trevissonbibiana influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma
AT becerrodebengoavallejoricardo influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma
AT sevillanodavid influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma
AT gonzaleznatalia influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma
AT losaiglesiasmartaelena influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma
AT lopezlopezdaniel influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma
AT alouluis influenceofsexualdimorphismaginganddifferentialcellcaptureefficiencyofbloodseparationsystemsonthequalityofplateletrichplasma