Cargando…

Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice

Retinitis pigmentosa (RP) is an inherited eye disorder which triggers a cascade of retinal disorders leading to photoreceptor cell death and for which there is currently no effective treatment. The purpose of this research was to study whether ocular administration of a solution of progesterone (PG)...

Descripción completa

Detalles Bibliográficos
Autores principales: Alambiaga-Caravaca, Adrián M., Cantó, Antolín, Rodilla, Vicent, Miranda, María, López-Castellano, Alicia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953546/
https://www.ncbi.nlm.nih.gov/pubmed/35337126
http://dx.doi.org/10.3390/ph15030328
_version_ 1784675877785698304
author Alambiaga-Caravaca, Adrián M.
Cantó, Antolín
Rodilla, Vicent
Miranda, María
López-Castellano, Alicia
author_facet Alambiaga-Caravaca, Adrián M.
Cantó, Antolín
Rodilla, Vicent
Miranda, María
López-Castellano, Alicia
author_sort Alambiaga-Caravaca, Adrián M.
collection PubMed
description Retinitis pigmentosa (RP) is an inherited eye disorder which triggers a cascade of retinal disorders leading to photoreceptor cell death and for which there is currently no effective treatment. The purpose of this research was to study whether ocular administration of a solution of progesterone (PG) in β-cyclodextrins (CD) could delay photoreceptor cell death and counteract the gliosis process in an animal model of RP (rds mice). The possible effect of PG reaching the contralateral eye through the circulatory system was also evaluated. Finally, this research discusses and evaluates the diffusion of the drug from possible topical formulations for ocular administration of PG. A group of rds mice received one drop of a solution of PG in CD every 12 h for 10 days to the left eye, while the right eye was left untreated. Another group of rds mice (control) received the drug vehicle (PBS) on the left eye and, again, the right eye was left untreated. Once the treatment was finished on postnatal day 21, the animals were euthanized and histological immunofluorescence studies (TUNEL, GFAP, and DAPI staining) were carried out. Our results showed that the administration of a solution of PG in CD (CD-PG) as drops significantly decreased cell death and inflammation in the retina of the PG-treated eyes of rds mice. No effect was seen in the contralateral eye from PG that may have entered systemic circulation. In conclusion, CD-PG applied topically as drops to the eye decreases photoreceptor cell death in the early stages of RP, delaying vision loss and decreasing gliosis.
format Online
Article
Text
id pubmed-8953546
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89535462022-03-26 Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice Alambiaga-Caravaca, Adrián M. Cantó, Antolín Rodilla, Vicent Miranda, María López-Castellano, Alicia Pharmaceuticals (Basel) Article Retinitis pigmentosa (RP) is an inherited eye disorder which triggers a cascade of retinal disorders leading to photoreceptor cell death and for which there is currently no effective treatment. The purpose of this research was to study whether ocular administration of a solution of progesterone (PG) in β-cyclodextrins (CD) could delay photoreceptor cell death and counteract the gliosis process in an animal model of RP (rds mice). The possible effect of PG reaching the contralateral eye through the circulatory system was also evaluated. Finally, this research discusses and evaluates the diffusion of the drug from possible topical formulations for ocular administration of PG. A group of rds mice received one drop of a solution of PG in CD every 12 h for 10 days to the left eye, while the right eye was left untreated. Another group of rds mice (control) received the drug vehicle (PBS) on the left eye and, again, the right eye was left untreated. Once the treatment was finished on postnatal day 21, the animals were euthanized and histological immunofluorescence studies (TUNEL, GFAP, and DAPI staining) were carried out. Our results showed that the administration of a solution of PG in CD (CD-PG) as drops significantly decreased cell death and inflammation in the retina of the PG-treated eyes of rds mice. No effect was seen in the contralateral eye from PG that may have entered systemic circulation. In conclusion, CD-PG applied topically as drops to the eye decreases photoreceptor cell death in the early stages of RP, delaying vision loss and decreasing gliosis. MDPI 2022-03-09 /pmc/articles/PMC8953546/ /pubmed/35337126 http://dx.doi.org/10.3390/ph15030328 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alambiaga-Caravaca, Adrián M.
Cantó, Antolín
Rodilla, Vicent
Miranda, María
López-Castellano, Alicia
Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice
title Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice
title_full Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice
title_fullStr Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice
title_full_unstemmed Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice
title_short Topical Ocular Administration of Progesterone Decreases Photoreceptor Cell Death in Retinal Degeneration Slow (rds) Mice
title_sort topical ocular administration of progesterone decreases photoreceptor cell death in retinal degeneration slow (rds) mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953546/
https://www.ncbi.nlm.nih.gov/pubmed/35337126
http://dx.doi.org/10.3390/ph15030328
work_keys_str_mv AT alambiagacaravacaadrianm topicalocularadministrationofprogesteronedecreasesphotoreceptorcelldeathinretinaldegenerationslowrdsmice
AT cantoantolin topicalocularadministrationofprogesteronedecreasesphotoreceptorcelldeathinretinaldegenerationslowrdsmice
AT rodillavicent topicalocularadministrationofprogesteronedecreasesphotoreceptorcelldeathinretinaldegenerationslowrdsmice
AT mirandamaria topicalocularadministrationofprogesteronedecreasesphotoreceptorcelldeathinretinaldegenerationslowrdsmice
AT lopezcastellanoalicia topicalocularadministrationofprogesteronedecreasesphotoreceptorcelldeathinretinaldegenerationslowrdsmice