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MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve...

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Autores principales: Yang, Jing, Zhong, Wei, Yu, Qi, Zou, Jin, Gao, Yansha, Liu, Shuwu, Zhang, Songbai, Wang, Xiaoqiang, Lu, Limin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953677/
https://www.ncbi.nlm.nih.gov/pubmed/35335235
http://dx.doi.org/10.3390/molecules27061871
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author Yang, Jing
Zhong, Wei
Yu, Qi
Zou, Jin
Gao, Yansha
Liu, Shuwu
Zhang, Songbai
Wang, Xiaoqiang
Lu, Limin
author_facet Yang, Jing
Zhong, Wei
Yu, Qi
Zou, Jin
Gao, Yansha
Liu, Shuwu
Zhang, Songbai
Wang, Xiaoqiang
Lu, Limin
author_sort Yang, Jing
collection PubMed
description A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve the quantity of active sites and electronic conductivity. The aptamers (Apts) were able to immobilize on the MXene–AuNP modified electrode surface through Au–S interaction. Upon specifically binding with CAP with high affinity, the CAP–Apt complexes produced low conductivity on the aptasensor surface, leading to a decreased electrochemical signal. The resulting current change was quantitatively correlated with CAP concentration. Under optimized experimental conditions, the constructed aptasensor exhibited a good linear relationship within a wide range of 0.0001–10 nM and with a low detection limit of 0.03 pM for CAP. Moreover, the developed aptasensor has been applied to the determination of CAP concentration in honey samples with satisfactory results.
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spelling pubmed-89536772022-03-26 MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey Yang, Jing Zhong, Wei Yu, Qi Zou, Jin Gao, Yansha Liu, Shuwu Zhang, Songbai Wang, Xiaoqiang Lu, Limin Molecules Article A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve the quantity of active sites and electronic conductivity. The aptamers (Apts) were able to immobilize on the MXene–AuNP modified electrode surface through Au–S interaction. Upon specifically binding with CAP with high affinity, the CAP–Apt complexes produced low conductivity on the aptasensor surface, leading to a decreased electrochemical signal. The resulting current change was quantitatively correlated with CAP concentration. Under optimized experimental conditions, the constructed aptasensor exhibited a good linear relationship within a wide range of 0.0001–10 nM and with a low detection limit of 0.03 pM for CAP. Moreover, the developed aptasensor has been applied to the determination of CAP concentration in honey samples with satisfactory results. MDPI 2022-03-14 /pmc/articles/PMC8953677/ /pubmed/35335235 http://dx.doi.org/10.3390/molecules27061871 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Jing
Zhong, Wei
Yu, Qi
Zou, Jin
Gao, Yansha
Liu, Shuwu
Zhang, Songbai
Wang, Xiaoqiang
Lu, Limin
MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey
title MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey
title_full MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey
title_fullStr MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey
title_full_unstemmed MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey
title_short MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey
title_sort mxene–aunp-based electrochemical aptasensor for ultra-sensitive detection of chloramphenicol in honey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953677/
https://www.ncbi.nlm.nih.gov/pubmed/35335235
http://dx.doi.org/10.3390/molecules27061871
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