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Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?

Skeletal muscle is highly plastic and dynamically regulated by the body’s physical demands. This study aimed to determine the plasticity of skeletal muscle DNA methylation in response to 8 weeks of supervised exercise training in volunteers with a range of insulin sensitivities. We studied 13 sedent...

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Autores principales: Garcia, Luis A., Zapata-Bustos, Rocio, Day, Samantha E., Campos, Baltazar, Hamzaoui, Yassin, Wu, Linda, Leon, Alma D., Krentzel, Judith, Coletta, Richard L., De Filippis, Eleanna, Roust, Lori R., Mandarino, Lawrence J., Coletta, Dawn K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953782/
https://www.ncbi.nlm.nih.gov/pubmed/35323665
http://dx.doi.org/10.3390/metabo12030222
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author Garcia, Luis A.
Zapata-Bustos, Rocio
Day, Samantha E.
Campos, Baltazar
Hamzaoui, Yassin
Wu, Linda
Leon, Alma D.
Krentzel, Judith
Coletta, Richard L.
De Filippis, Eleanna
Roust, Lori R.
Mandarino, Lawrence J.
Coletta, Dawn K.
author_facet Garcia, Luis A.
Zapata-Bustos, Rocio
Day, Samantha E.
Campos, Baltazar
Hamzaoui, Yassin
Wu, Linda
Leon, Alma D.
Krentzel, Judith
Coletta, Richard L.
De Filippis, Eleanna
Roust, Lori R.
Mandarino, Lawrence J.
Coletta, Dawn K.
author_sort Garcia, Luis A.
collection PubMed
description Skeletal muscle is highly plastic and dynamically regulated by the body’s physical demands. This study aimed to determine the plasticity of skeletal muscle DNA methylation in response to 8 weeks of supervised exercise training in volunteers with a range of insulin sensitivities. We studied 13 sedentary participants and performed euglycemic hyperinsulinemic clamps with basal vastus lateralis muscle biopsies and peak aerobic activity (VO2 peak) tests before and after training. We extracted DNA from the muscle biopsies and performed global methylation using Illumina’s Methylation EPIC 850K BeadChip. Training significantly increased peak aerobic capacity and insulin-stimulated glucose disposal. Fasting serum insulin and insulin levels during the steady state of the clamp were significantly lower post-training. Insulin clearance rates during the clamp increased following the training. We identified 13 increased and 90 decreased differentially methylated cytosines (DMCs) in response to 8 weeks of training. Of the 13 increased DMCs, 2 were within the following genes, FSTL3, and RP11-624M8.1. Of the 90 decreased DMCs, 9 were within the genes CNGA1, FCGR2A, KIF21A, MEIS1, NT5DC1, OR4D1, PRPF4B, SLC26A7, and ZNF280C. Moreover, pathway analysis showed an enrichment in metabolic and actin-cytoskeleton pathways for the decreased DMCs, and for the increased DMCs, an enrichment in signal-dependent regulation of myogenesis, NOTCH2 activation and transmission, and SMAD2/3: SMAD4 transcriptional activity pathways. Our findings showed that 8 weeks of exercise training alters skeletal muscle DNA methylation of specific genes and pathways in people with varying degrees of insulin sensitivity.
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spelling pubmed-89537822022-03-26 Can Exercise Training Alter Human Skeletal Muscle DNA Methylation? Garcia, Luis A. Zapata-Bustos, Rocio Day, Samantha E. Campos, Baltazar Hamzaoui, Yassin Wu, Linda Leon, Alma D. Krentzel, Judith Coletta, Richard L. De Filippis, Eleanna Roust, Lori R. Mandarino, Lawrence J. Coletta, Dawn K. Metabolites Article Skeletal muscle is highly plastic and dynamically regulated by the body’s physical demands. This study aimed to determine the plasticity of skeletal muscle DNA methylation in response to 8 weeks of supervised exercise training in volunteers with a range of insulin sensitivities. We studied 13 sedentary participants and performed euglycemic hyperinsulinemic clamps with basal vastus lateralis muscle biopsies and peak aerobic activity (VO2 peak) tests before and after training. We extracted DNA from the muscle biopsies and performed global methylation using Illumina’s Methylation EPIC 850K BeadChip. Training significantly increased peak aerobic capacity and insulin-stimulated glucose disposal. Fasting serum insulin and insulin levels during the steady state of the clamp were significantly lower post-training. Insulin clearance rates during the clamp increased following the training. We identified 13 increased and 90 decreased differentially methylated cytosines (DMCs) in response to 8 weeks of training. Of the 13 increased DMCs, 2 were within the following genes, FSTL3, and RP11-624M8.1. Of the 90 decreased DMCs, 9 were within the genes CNGA1, FCGR2A, KIF21A, MEIS1, NT5DC1, OR4D1, PRPF4B, SLC26A7, and ZNF280C. Moreover, pathway analysis showed an enrichment in metabolic and actin-cytoskeleton pathways for the decreased DMCs, and for the increased DMCs, an enrichment in signal-dependent regulation of myogenesis, NOTCH2 activation and transmission, and SMAD2/3: SMAD4 transcriptional activity pathways. Our findings showed that 8 weeks of exercise training alters skeletal muscle DNA methylation of specific genes and pathways in people with varying degrees of insulin sensitivity. MDPI 2022-03-02 /pmc/articles/PMC8953782/ /pubmed/35323665 http://dx.doi.org/10.3390/metabo12030222 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia, Luis A.
Zapata-Bustos, Rocio
Day, Samantha E.
Campos, Baltazar
Hamzaoui, Yassin
Wu, Linda
Leon, Alma D.
Krentzel, Judith
Coletta, Richard L.
De Filippis, Eleanna
Roust, Lori R.
Mandarino, Lawrence J.
Coletta, Dawn K.
Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?
title Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?
title_full Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?
title_fullStr Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?
title_full_unstemmed Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?
title_short Can Exercise Training Alter Human Skeletal Muscle DNA Methylation?
title_sort can exercise training alter human skeletal muscle dna methylation?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953782/
https://www.ncbi.nlm.nih.gov/pubmed/35323665
http://dx.doi.org/10.3390/metabo12030222
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