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α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition

Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short α/β-peptide tags with high affinity to...

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Detalles Bibliográficos
Autores principales: Hetényi, Anasztázia, Szabó, Enikő, Imre, Norbert, Bhaumik, Kaushik Nath, Tököli, Attila, Füzesi, Tamás, Hollandi, Réka, Horvath, Peter, Czibula, Ágnes, Monostori, Éva, Deli, Mária A., Martinek, Tamás A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953856/
https://www.ncbi.nlm.nih.gov/pubmed/35335956
http://dx.doi.org/10.3390/pharmaceutics14030580
Descripción
Sumario:Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short α/β-peptide tags with high affinity to the lipid raft-associated ganglioside GM1. These sequences induce effective internalization of the attached immunoglobulin cargo. The structural requirements of the GM1-peptide interaction are presented, and the importance of the membrane components are shown. The results contribute to the development of a receptor-based cell delivery platform.