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Metabonomics Analysis of Stem Extracts from Dalbergia sissoo

Dalbergia sissoo is a woody plant with economic and medicinal value. As the pharmacological qualities and properties of the wood from this plant primarily depend on its extractives, in this study, the metabolomic analysis of extractives from its stems was carried out using UPLC-MS/MS. A total of 735...

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Autores principales: Li, Mengxue, Liu, Mengying, Wang, Bingyi, Shi, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953952/
https://www.ncbi.nlm.nih.gov/pubmed/35335342
http://dx.doi.org/10.3390/molecules27061982
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author Li, Mengxue
Liu, Mengying
Wang, Bingyi
Shi, Lei
author_facet Li, Mengxue
Liu, Mengying
Wang, Bingyi
Shi, Lei
author_sort Li, Mengxue
collection PubMed
description Dalbergia sissoo is a woody plant with economic and medicinal value. As the pharmacological qualities and properties of the wood from this plant primarily depend on its extractives, in this study, the metabolomic analysis of extractives from its stems was carried out using UPLC-MS/MS. A total of 735 metabolites were detected from two groups of samples, heartwood and sapwood, with the largest number of terpenoids in type and the largest number of flavonoids in quantity. The PCA and cluster analysis showed significant differences in the metabolite composition between the two groups. The differential metabolites were mainly organic oxygen compounds, flavonoids, and isoflavones. Among the 105 differential metabolites, 26 metabolites were significantly higher in relative content in sapwood than in heartwood, while the other 79 metabolites were significantly higher in relative content in heartwood than in sapwood. KEGG metabolic pathway enrichment analysis showed that these differential metabolites were mainly enriched in three metabolic pathways: Flavonoid biosynthesis, isoflavonoid biosynthesis, and flavonoid and flavonol biosynthesis. This study provides a reference for metabolomics studies in Dalbergia and other woody plants.
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spelling pubmed-89539522022-03-26 Metabonomics Analysis of Stem Extracts from Dalbergia sissoo Li, Mengxue Liu, Mengying Wang, Bingyi Shi, Lei Molecules Article Dalbergia sissoo is a woody plant with economic and medicinal value. As the pharmacological qualities and properties of the wood from this plant primarily depend on its extractives, in this study, the metabolomic analysis of extractives from its stems was carried out using UPLC-MS/MS. A total of 735 metabolites were detected from two groups of samples, heartwood and sapwood, with the largest number of terpenoids in type and the largest number of flavonoids in quantity. The PCA and cluster analysis showed significant differences in the metabolite composition between the two groups. The differential metabolites were mainly organic oxygen compounds, flavonoids, and isoflavones. Among the 105 differential metabolites, 26 metabolites were significantly higher in relative content in sapwood than in heartwood, while the other 79 metabolites were significantly higher in relative content in heartwood than in sapwood. KEGG metabolic pathway enrichment analysis showed that these differential metabolites were mainly enriched in three metabolic pathways: Flavonoid biosynthesis, isoflavonoid biosynthesis, and flavonoid and flavonol biosynthesis. This study provides a reference for metabolomics studies in Dalbergia and other woody plants. MDPI 2022-03-18 /pmc/articles/PMC8953952/ /pubmed/35335342 http://dx.doi.org/10.3390/molecules27061982 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Mengxue
Liu, Mengying
Wang, Bingyi
Shi, Lei
Metabonomics Analysis of Stem Extracts from Dalbergia sissoo
title Metabonomics Analysis of Stem Extracts from Dalbergia sissoo
title_full Metabonomics Analysis of Stem Extracts from Dalbergia sissoo
title_fullStr Metabonomics Analysis of Stem Extracts from Dalbergia sissoo
title_full_unstemmed Metabonomics Analysis of Stem Extracts from Dalbergia sissoo
title_short Metabonomics Analysis of Stem Extracts from Dalbergia sissoo
title_sort metabonomics analysis of stem extracts from dalbergia sissoo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8953952/
https://www.ncbi.nlm.nih.gov/pubmed/35335342
http://dx.doi.org/10.3390/molecules27061982
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