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Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice
The primary aim of this study was to evaluate the efficacy of phage against mastitis induced by drug-resistant S. aureus in a mouse model. In this study, five S. aureus phages—4086-1, 4086-2, 4086-3, 4086-4, and 4086-6—were isolated from milk samples secreted by mastitis cows. Transmission electron...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954217/ https://www.ncbi.nlm.nih.gov/pubmed/35337027 http://dx.doi.org/10.3390/v14030620 |
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author | Teng, Fei Xiong, Xiaoyu Zhang, Songsong Li, Guiwei Wang, Ruichong Zhang, Lanlan Wang, Xiaona Zhou, Han Li, Jiaxuan Li, Yijing Jiang, Yanping Cui, Wen Tang, Lijie Wang, Li Qiao, Xinyuan |
author_facet | Teng, Fei Xiong, Xiaoyu Zhang, Songsong Li, Guiwei Wang, Ruichong Zhang, Lanlan Wang, Xiaona Zhou, Han Li, Jiaxuan Li, Yijing Jiang, Yanping Cui, Wen Tang, Lijie Wang, Li Qiao, Xinyuan |
author_sort | Teng, Fei |
collection | PubMed |
description | The primary aim of this study was to evaluate the efficacy of phage against mastitis induced by drug-resistant S. aureus in a mouse model. In this study, five S. aureus phages—4086-1, 4086-2, 4086-3, 4086-4, and 4086-6—were isolated from milk samples secreted by mastitis cows. Transmission electron microscopy showed that all the five phages had icosahedral heads and short non-contractile tails, which are typical characteristics of the family Podoviridae. All these phages were species-specific against S. aureus. The one-step growth curve showed a short latency period (10–20 min) and high burst size (up to 400 PFU/infected cell). To evaluate the effectiveness of the phage 4086-1 in the treatment against mastitis, a mouse model of mastitis was challenged with drug-resistant S. aureus. The results showed the proliferation of S. aureus in the mammary glands was significantly inhibited after treating by phage 4086-1. The concentrations of TNF-α and IL-6 decreased significantly, which demonstrated the phages could effectively alleviate the inflammatory responses. Furthermore, the histopathological analysis showed that inflammatory infiltration in the mammary glands was significantly reduced. These results demonstrate that phage may be a promising alternative therapy against mastitis caused by drug-resistant S. aureus. |
format | Online Article Text |
id | pubmed-8954217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89542172022-03-26 Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice Teng, Fei Xiong, Xiaoyu Zhang, Songsong Li, Guiwei Wang, Ruichong Zhang, Lanlan Wang, Xiaona Zhou, Han Li, Jiaxuan Li, Yijing Jiang, Yanping Cui, Wen Tang, Lijie Wang, Li Qiao, Xinyuan Viruses Article The primary aim of this study was to evaluate the efficacy of phage against mastitis induced by drug-resistant S. aureus in a mouse model. In this study, five S. aureus phages—4086-1, 4086-2, 4086-3, 4086-4, and 4086-6—were isolated from milk samples secreted by mastitis cows. Transmission electron microscopy showed that all the five phages had icosahedral heads and short non-contractile tails, which are typical characteristics of the family Podoviridae. All these phages were species-specific against S. aureus. The one-step growth curve showed a short latency period (10–20 min) and high burst size (up to 400 PFU/infected cell). To evaluate the effectiveness of the phage 4086-1 in the treatment against mastitis, a mouse model of mastitis was challenged with drug-resistant S. aureus. The results showed the proliferation of S. aureus in the mammary glands was significantly inhibited after treating by phage 4086-1. The concentrations of TNF-α and IL-6 decreased significantly, which demonstrated the phages could effectively alleviate the inflammatory responses. Furthermore, the histopathological analysis showed that inflammatory infiltration in the mammary glands was significantly reduced. These results demonstrate that phage may be a promising alternative therapy against mastitis caused by drug-resistant S. aureus. MDPI 2022-03-16 /pmc/articles/PMC8954217/ /pubmed/35337027 http://dx.doi.org/10.3390/v14030620 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Teng, Fei Xiong, Xiaoyu Zhang, Songsong Li, Guiwei Wang, Ruichong Zhang, Lanlan Wang, Xiaona Zhou, Han Li, Jiaxuan Li, Yijing Jiang, Yanping Cui, Wen Tang, Lijie Wang, Li Qiao, Xinyuan Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice |
title | Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice |
title_full | Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice |
title_fullStr | Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice |
title_full_unstemmed | Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice |
title_short | Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice |
title_sort | efficacy assessment of phage therapy in treating staphylococcus aureus-induced mastitis in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954217/ https://www.ncbi.nlm.nih.gov/pubmed/35337027 http://dx.doi.org/10.3390/v14030620 |
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