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Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING

In recent decades, the demand for biomedical imaging tools has grown very rapidly as a key feature for biomedical research and diagnostic applications. Particularly, fluorescence imaging has gained increased attention as a non-invasive, inexpensive technique that allows real-time imaging. However, t...

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Autores principales: Calatayud, David G., Jardiel, Teresa, Cordero-Oyonarte, Erica, Caballero, Amador C., Villegas, Marina, Valle-Noguera, Ana, Cruz-Adalia, Aranzazu, Peiteado, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954243/
https://www.ncbi.nlm.nih.gov/pubmed/35328831
http://dx.doi.org/10.3390/ijms23063410
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author Calatayud, David G.
Jardiel, Teresa
Cordero-Oyonarte, Erica
Caballero, Amador C.
Villegas, Marina
Valle-Noguera, Ana
Cruz-Adalia, Aranzazu
Peiteado, Marco
author_facet Calatayud, David G.
Jardiel, Teresa
Cordero-Oyonarte, Erica
Caballero, Amador C.
Villegas, Marina
Valle-Noguera, Ana
Cruz-Adalia, Aranzazu
Peiteado, Marco
author_sort Calatayud, David G.
collection PubMed
description In recent decades, the demand for biomedical imaging tools has grown very rapidly as a key feature for biomedical research and diagnostic applications. Particularly, fluorescence imaging has gained increased attention as a non-invasive, inexpensive technique that allows real-time imaging. However, tissue auto-fluorescence under external illumination, together with a weak tissue penetration of low wavelength excitation light, largely restricts the application of the technique. Accordingly, new types of fluorescent labels are currently being investigated and, in this search, phosphorescent nanoparticles promise great potential, as they combine the interesting size-dependent properties of nanoscale materials with a long-lasting phosphorescence-type emission that allows optical imaging well after excitation (so avoiding autofluorescence). In this work, core-shell structures consisting of SrAlO:Eu,Dy luminescent cores encapsulated within a biocompatible silica shell were prepared, showing a green persistent phosphorescence with an afterglow time of more than 1000 s. A high-energy ball milling procedure was used to reduce the size of the starting phosphors to a size suitable for cellular uptake, while the silica coating was produced by a reverse micelle methodology that eventually allows the excitation and emission light to pass efficiently through the shell. Confocal fluorescence microscopy using HeLa cancer cells confirmed the potential of the all-ceramic composites produced as feasible labels for in vitro optical imaging.
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spelling pubmed-89542432022-03-26 Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING Calatayud, David G. Jardiel, Teresa Cordero-Oyonarte, Erica Caballero, Amador C. Villegas, Marina Valle-Noguera, Ana Cruz-Adalia, Aranzazu Peiteado, Marco Int J Mol Sci Article In recent decades, the demand for biomedical imaging tools has grown very rapidly as a key feature for biomedical research and diagnostic applications. Particularly, fluorescence imaging has gained increased attention as a non-invasive, inexpensive technique that allows real-time imaging. However, tissue auto-fluorescence under external illumination, together with a weak tissue penetration of low wavelength excitation light, largely restricts the application of the technique. Accordingly, new types of fluorescent labels are currently being investigated and, in this search, phosphorescent nanoparticles promise great potential, as they combine the interesting size-dependent properties of nanoscale materials with a long-lasting phosphorescence-type emission that allows optical imaging well after excitation (so avoiding autofluorescence). In this work, core-shell structures consisting of SrAlO:Eu,Dy luminescent cores encapsulated within a biocompatible silica shell were prepared, showing a green persistent phosphorescence with an afterglow time of more than 1000 s. A high-energy ball milling procedure was used to reduce the size of the starting phosphors to a size suitable for cellular uptake, while the silica coating was produced by a reverse micelle methodology that eventually allows the excitation and emission light to pass efficiently through the shell. Confocal fluorescence microscopy using HeLa cancer cells confirmed the potential of the all-ceramic composites produced as feasible labels for in vitro optical imaging. MDPI 2022-03-21 /pmc/articles/PMC8954243/ /pubmed/35328831 http://dx.doi.org/10.3390/ijms23063410 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Calatayud, David G.
Jardiel, Teresa
Cordero-Oyonarte, Erica
Caballero, Amador C.
Villegas, Marina
Valle-Noguera, Ana
Cruz-Adalia, Aranzazu
Peiteado, Marco
Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
title Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
title_full Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
title_fullStr Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
title_full_unstemmed Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
title_short Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
title_sort biocompatible probes based on rare-earth doped strontium aluminates with long-lasting phosphorescent properties for in vitro optical imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954243/
https://www.ncbi.nlm.nih.gov/pubmed/35328831
http://dx.doi.org/10.3390/ijms23063410
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