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Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L

Rotaviruses infect humans and animals and are a main cause of diarrhea. They are non-enveloped viruses with a genome of 11 double-stranded RNA segments. Based on genome analysis and amino acid sequence identities of the capsid protein VP6, the rotavirus species A to J (RVA-RVJ) have been defined so...

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Autores principales: Johne, Reimar, Schilling-Loeffler, Katja, Ulrich, Rainer G., Tausch, Simon H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954357/
https://www.ncbi.nlm.nih.gov/pubmed/35336869
http://dx.doi.org/10.3390/v14030462
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author Johne, Reimar
Schilling-Loeffler, Katja
Ulrich, Rainer G.
Tausch, Simon H.
author_facet Johne, Reimar
Schilling-Loeffler, Katja
Ulrich, Rainer G.
Tausch, Simon H.
author_sort Johne, Reimar
collection PubMed
description Rotaviruses infect humans and animals and are a main cause of diarrhea. They are non-enveloped viruses with a genome of 11 double-stranded RNA segments. Based on genome analysis and amino acid sequence identities of the capsid protein VP6, the rotavirus species A to J (RVA-RVJ) have been defined so far. In addition, rotaviruses putatively assigned to the novel rotavirus species K (RVK) and L (RVL) have been recently identified in common shrews (Sorex araneus), based on partial genome sequences. Here, the complete genome sequence of strain KS14/0241, a prototype strain of RVL, is presented. The deduced amino acid sequence for VP6 of this strain shows only up to 47% identity to that of RVA to RVJ reference strains. Phylogenetic analyses indicate a clustering separated from the established rotavirus species for all 11 genome segments of RVL, with the closest relationship to RVH and RVJ within the phylogenetic RVB-like clade. The non-coding genome segment termini of RVL showed conserved sequences at the 5′-end (positive-sense RNA strand), which are common to all rotaviruses, and those conserved among the RVB-like clade at the 3′-end. The results are consistent with a classification of the virus into a novel rotavirus species L.
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spelling pubmed-89543572022-03-26 Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L Johne, Reimar Schilling-Loeffler, Katja Ulrich, Rainer G. Tausch, Simon H. Viruses Article Rotaviruses infect humans and animals and are a main cause of diarrhea. They are non-enveloped viruses with a genome of 11 double-stranded RNA segments. Based on genome analysis and amino acid sequence identities of the capsid protein VP6, the rotavirus species A to J (RVA-RVJ) have been defined so far. In addition, rotaviruses putatively assigned to the novel rotavirus species K (RVK) and L (RVL) have been recently identified in common shrews (Sorex araneus), based on partial genome sequences. Here, the complete genome sequence of strain KS14/0241, a prototype strain of RVL, is presented. The deduced amino acid sequence for VP6 of this strain shows only up to 47% identity to that of RVA to RVJ reference strains. Phylogenetic analyses indicate a clustering separated from the established rotavirus species for all 11 genome segments of RVL, with the closest relationship to RVH and RVJ within the phylogenetic RVB-like clade. The non-coding genome segment termini of RVL showed conserved sequences at the 5′-end (positive-sense RNA strand), which are common to all rotaviruses, and those conserved among the RVB-like clade at the 3′-end. The results are consistent with a classification of the virus into a novel rotavirus species L. MDPI 2022-02-24 /pmc/articles/PMC8954357/ /pubmed/35336869 http://dx.doi.org/10.3390/v14030462 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Johne, Reimar
Schilling-Loeffler, Katja
Ulrich, Rainer G.
Tausch, Simon H.
Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
title Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
title_full Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
title_fullStr Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
title_full_unstemmed Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
title_short Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
title_sort whole genome sequence analysis of a prototype strain of the novel putative rotavirus species l
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954357/
https://www.ncbi.nlm.nih.gov/pubmed/35336869
http://dx.doi.org/10.3390/v14030462
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