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Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
Rotaviruses infect humans and animals and are a main cause of diarrhea. They are non-enveloped viruses with a genome of 11 double-stranded RNA segments. Based on genome analysis and amino acid sequence identities of the capsid protein VP6, the rotavirus species A to J (RVA-RVJ) have been defined so...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954357/ https://www.ncbi.nlm.nih.gov/pubmed/35336869 http://dx.doi.org/10.3390/v14030462 |
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author | Johne, Reimar Schilling-Loeffler, Katja Ulrich, Rainer G. Tausch, Simon H. |
author_facet | Johne, Reimar Schilling-Loeffler, Katja Ulrich, Rainer G. Tausch, Simon H. |
author_sort | Johne, Reimar |
collection | PubMed |
description | Rotaviruses infect humans and animals and are a main cause of diarrhea. They are non-enveloped viruses with a genome of 11 double-stranded RNA segments. Based on genome analysis and amino acid sequence identities of the capsid protein VP6, the rotavirus species A to J (RVA-RVJ) have been defined so far. In addition, rotaviruses putatively assigned to the novel rotavirus species K (RVK) and L (RVL) have been recently identified in common shrews (Sorex araneus), based on partial genome sequences. Here, the complete genome sequence of strain KS14/0241, a prototype strain of RVL, is presented. The deduced amino acid sequence for VP6 of this strain shows only up to 47% identity to that of RVA to RVJ reference strains. Phylogenetic analyses indicate a clustering separated from the established rotavirus species for all 11 genome segments of RVL, with the closest relationship to RVH and RVJ within the phylogenetic RVB-like clade. The non-coding genome segment termini of RVL showed conserved sequences at the 5′-end (positive-sense RNA strand), which are common to all rotaviruses, and those conserved among the RVB-like clade at the 3′-end. The results are consistent with a classification of the virus into a novel rotavirus species L. |
format | Online Article Text |
id | pubmed-8954357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89543572022-03-26 Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L Johne, Reimar Schilling-Loeffler, Katja Ulrich, Rainer G. Tausch, Simon H. Viruses Article Rotaviruses infect humans and animals and are a main cause of diarrhea. They are non-enveloped viruses with a genome of 11 double-stranded RNA segments. Based on genome analysis and amino acid sequence identities of the capsid protein VP6, the rotavirus species A to J (RVA-RVJ) have been defined so far. In addition, rotaviruses putatively assigned to the novel rotavirus species K (RVK) and L (RVL) have been recently identified in common shrews (Sorex araneus), based on partial genome sequences. Here, the complete genome sequence of strain KS14/0241, a prototype strain of RVL, is presented. The deduced amino acid sequence for VP6 of this strain shows only up to 47% identity to that of RVA to RVJ reference strains. Phylogenetic analyses indicate a clustering separated from the established rotavirus species for all 11 genome segments of RVL, with the closest relationship to RVH and RVJ within the phylogenetic RVB-like clade. The non-coding genome segment termini of RVL showed conserved sequences at the 5′-end (positive-sense RNA strand), which are common to all rotaviruses, and those conserved among the RVB-like clade at the 3′-end. The results are consistent with a classification of the virus into a novel rotavirus species L. MDPI 2022-02-24 /pmc/articles/PMC8954357/ /pubmed/35336869 http://dx.doi.org/10.3390/v14030462 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Johne, Reimar Schilling-Loeffler, Katja Ulrich, Rainer G. Tausch, Simon H. Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L |
title | Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L |
title_full | Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L |
title_fullStr | Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L |
title_full_unstemmed | Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L |
title_short | Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L |
title_sort | whole genome sequence analysis of a prototype strain of the novel putative rotavirus species l |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954357/ https://www.ncbi.nlm.nih.gov/pubmed/35336869 http://dx.doi.org/10.3390/v14030462 |
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