Cargando…

A New Approach to Supramolecular Structure Determination in Pharmaceutical Preparation of Self-Assembling Peptides: A Case Study of Lanreotide Autogel

The supramolecular structure in peptides’ prolonged-released gel formulations is the most critical parameter for the determination of the pharmaceutical profile of the drug. Here, we report our investigation on lanreotide Autogel as a case study. For the first time, we describe the use of the pulsed...

Descripción completa

Detalles Bibliográficos
Autores principales: Grimaldi, Manuela, Santoro, Angelo, Buonocore, Michela, Crivaro, Claudio, Funicello, Nicola, Sublimi Saponetti, Matilde, Ripoli, Cristina, Rodriquez, Manuela, De Pasquale, Salvatore, Bobba, Fabrizio, Ferrazzano, Lucia, Cabri, Walter, D’Ursi, Anna Maria, Ricci, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954372/
https://www.ncbi.nlm.nih.gov/pubmed/35336055
http://dx.doi.org/10.3390/pharmaceutics14030681
Descripción
Sumario:The supramolecular structure in peptides’ prolonged-released gel formulations is the most critical parameter for the determination of the pharmaceutical profile of the drug. Here, we report our investigation on lanreotide Autogel as a case study. For the first time, we describe the use of the pulsed field gradient (PFG) diffusion-ordered spectroscopy (DOSY) magic-angle spinning NMR to characterize the supramolecular self-assembly and molecular mobility of different samples of lanreotide Autogel formulations prepared according to different formulation protocols. The diffusion coefficient was used to calculate the hydrodynamic radii of supramolecular assemblies and build relative molecular models. DOSY data were integrated with NMR imaging (MRI) measurements and atomic force microscopy (AFM) imaging.