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The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination

Accurate host identification is paramount to understand disease epidemiology and to apply appropriate control measures. This is especially important for multi-host pathogens such as the rabies virus, a major and almost invariably fatal zoonosis that has mobilized unanimous engagement at an internati...

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Autores principales: De Benedictis, Paola, Leopardi, Stefania, Markotter, Wanda, Velasco-Villa, Andres
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954416/
https://www.ncbi.nlm.nih.gov/pubmed/35336899
http://dx.doi.org/10.3390/v14030492
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author De Benedictis, Paola
Leopardi, Stefania
Markotter, Wanda
Velasco-Villa, Andres
author_facet De Benedictis, Paola
Leopardi, Stefania
Markotter, Wanda
Velasco-Villa, Andres
author_sort De Benedictis, Paola
collection PubMed
description Accurate host identification is paramount to understand disease epidemiology and to apply appropriate control measures. This is especially important for multi-host pathogens such as the rabies virus, a major and almost invariably fatal zoonosis that has mobilized unanimous engagement at an international level towards the final goal of zero human deaths due to canine rabies. Currently, diagnostic laboratories implement a standardized identification using taxonomic keys. However, this method is challenged by high and undiscovered biodiversity, decomposition of carcasses and subjective misevaluation, as has been attested to by findings from a cohort of 242 archived specimens collected across Sub-Saharan Africa and submitted for rabies diagnosis. We applied two simple and cheap methods targeting the Cytochrome b and Cytochrome c oxidase subunit I to confirm the initial classification. We therefore suggest prioritizing a standardized protocol that includes, as a first step, the implementation of taxonomic keys at a family or subfamily level, followed by the molecular characterization of the host species.
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spelling pubmed-89544162022-03-26 The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination De Benedictis, Paola Leopardi, Stefania Markotter, Wanda Velasco-Villa, Andres Viruses Perspective Accurate host identification is paramount to understand disease epidemiology and to apply appropriate control measures. This is especially important for multi-host pathogens such as the rabies virus, a major and almost invariably fatal zoonosis that has mobilized unanimous engagement at an international level towards the final goal of zero human deaths due to canine rabies. Currently, diagnostic laboratories implement a standardized identification using taxonomic keys. However, this method is challenged by high and undiscovered biodiversity, decomposition of carcasses and subjective misevaluation, as has been attested to by findings from a cohort of 242 archived specimens collected across Sub-Saharan Africa and submitted for rabies diagnosis. We applied two simple and cheap methods targeting the Cytochrome b and Cytochrome c oxidase subunit I to confirm the initial classification. We therefore suggest prioritizing a standardized protocol that includes, as a first step, the implementation of taxonomic keys at a family or subfamily level, followed by the molecular characterization of the host species. MDPI 2022-02-28 /pmc/articles/PMC8954416/ /pubmed/35336899 http://dx.doi.org/10.3390/v14030492 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
De Benedictis, Paola
Leopardi, Stefania
Markotter, Wanda
Velasco-Villa, Andres
The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination
title The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination
title_full The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination
title_fullStr The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination
title_full_unstemmed The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination
title_short The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination
title_sort importance of accurate host species identification in the framework of rabies surveillance, control and elimination
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954416/
https://www.ncbi.nlm.nih.gov/pubmed/35336899
http://dx.doi.org/10.3390/v14030492
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