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Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus

Aedes albopictus is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific viruses, which can...

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Autores principales: Batool, Khadija, Xiao, Jie, Xu, Ye, Yang, Ting, Tao, Peiwen, Zhao, Siyu, Chen, Jiao, Alam, Intikhab, Xie, Yugu, Gu, Jinbao, Chen, Xiaoguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954509/
https://www.ncbi.nlm.nih.gov/pubmed/35336882
http://dx.doi.org/10.3390/v14030475
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author Batool, Khadija
Xiao, Jie
Xu, Ye
Yang, Ting
Tao, Peiwen
Zhao, Siyu
Chen, Jiao
Alam, Intikhab
Xie, Yugu
Gu, Jinbao
Chen, Xiaoguang
author_facet Batool, Khadija
Xiao, Jie
Xu, Ye
Yang, Ting
Tao, Peiwen
Zhao, Siyu
Chen, Jiao
Alam, Intikhab
Xie, Yugu
Gu, Jinbao
Chen, Xiaoguang
author_sort Batool, Khadija
collection PubMed
description Aedes albopictus is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific viruses, which can reduce the capacity of isolated vectors and decrease mosquito-borne viral disease transmission. However, their variable pathogenicity restricts their commercial use. In the present study, we developed a series of novel larvicide oil suspensions (denoted Bacillus thuringiensis (Bti) oil, Ae. albopictus densovirus (AalDV-5) oil, and a mixture of AalDV-5+Bti oil), which were tested against Ae. albopictus larvae under experimental semi-field and open-field conditions. The effect of AalDV-5 on non-target species was also evaluated. The combined effect of AalDV-5+Bti was greater than that of individual toxins and was longer lasting and more persistent compared with the laboratory AalDV-5 virus strain. The virus was quantified on a weekly basis by quantitative polymerase chain reaction (qPCR) and was persistently detected in rearing water as well as in dead larvae. Wildtype densovirus is not pathogenic to non-target organisms. The present findings confirm the improved effect of a mixed microbial suspension (AalDV-5+Bti oil) larvicide against Ae. albopictus. The development and testing of these products will enable better control of the vector mosquitoes.
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spelling pubmed-89545092022-03-26 Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus Batool, Khadija Xiao, Jie Xu, Ye Yang, Ting Tao, Peiwen Zhao, Siyu Chen, Jiao Alam, Intikhab Xie, Yugu Gu, Jinbao Chen, Xiaoguang Viruses Article Aedes albopictus is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific viruses, which can reduce the capacity of isolated vectors and decrease mosquito-borne viral disease transmission. However, their variable pathogenicity restricts their commercial use. In the present study, we developed a series of novel larvicide oil suspensions (denoted Bacillus thuringiensis (Bti) oil, Ae. albopictus densovirus (AalDV-5) oil, and a mixture of AalDV-5+Bti oil), which were tested against Ae. albopictus larvae under experimental semi-field and open-field conditions. The effect of AalDV-5 on non-target species was also evaluated. The combined effect of AalDV-5+Bti was greater than that of individual toxins and was longer lasting and more persistent compared with the laboratory AalDV-5 virus strain. The virus was quantified on a weekly basis by quantitative polymerase chain reaction (qPCR) and was persistently detected in rearing water as well as in dead larvae. Wildtype densovirus is not pathogenic to non-target organisms. The present findings confirm the improved effect of a mixed microbial suspension (AalDV-5+Bti oil) larvicide against Ae. albopictus. The development and testing of these products will enable better control of the vector mosquitoes. MDPI 2022-02-25 /pmc/articles/PMC8954509/ /pubmed/35336882 http://dx.doi.org/10.3390/v14030475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Batool, Khadija
Xiao, Jie
Xu, Ye
Yang, Ting
Tao, Peiwen
Zhao, Siyu
Chen, Jiao
Alam, Intikhab
Xie, Yugu
Gu, Jinbao
Chen, Xiaoguang
Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
title Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
title_full Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
title_fullStr Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
title_full_unstemmed Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
title_short Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
title_sort densovirus oil suspension significantly improves the efficacy and duration of larvicidal activity against aedes albopictus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954509/
https://www.ncbi.nlm.nih.gov/pubmed/35336882
http://dx.doi.org/10.3390/v14030475
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