Larvicidal Activity of Carbon Black against the Yellow Fever Mosquito Aedes aegypti

SIMPLE SUMMARY: Nanoparticles have previously shown potential to control mosquito vectors. The present study examined whether carbon black, an industrial source of carbon-based nanoparticles (CNPs), was toxic to larvae of the yellow fever mosquito (Aedes aegypti). We found that exposing the first developmental stages of mosquito larvae to a modified form of carbon black EMPEROR(®) 1800 (E1800), caused concentration-dependent mortality within 48 h of exposure; however, the development of larvae exposed to sub-lethal concentrations of E1800 was not disrupted. Analyses of E1800 suspensions suggest this carbon black forms CNPs that coalesce into larger aggregations. Microscopic observations of dead larvae showed the presence of CNP aggregations in the digestive tract and on external structures associated with swimming, breathing, and food uptake. Our results suggest carbon black is a source of CNPs that may have potential use for treating sources of standing water that mosquitoes use as breeding sites. ABSTRACT: The yellow fever mosquito Aedes aegypti is one of the deadliest animals on the planet because it transmits several medically important arboviruses, including Zika, chikungunya, dengue, and yellow fever. Carbon-based nanoparticles (CNPs) derived from natural sources have previously been shown to have toxic effects on mosquito larvae and offer a potential alternative to chemical insecticides such as pyrethroids, for which mosquitoes have evolved resistance. However, CNPs derived from industrial sources, such as carbon black, have not previously been evaluated as larvicides. Here, we evaluate the effects of a commercially-available carbon black, EMPEROR(®) 1800 (E1800), on mortality and development of pyrethroid-susceptible (PS) and pyrethroid-resistant (PR) strains of Ae. aegypti. We found that E1800 exhibited concentration-dependent mortality against 1st instar larvae of both strains within the first 120 h after exposure, but after this period, surviving larvae did not show delays in their development to adults. Physical characterization of E1800 suspensions suggests that they form primary particles of ~30 nm in diameter that fuse into fundamental aggregates of ~170 nm in diameter. Notably, larvae treated with E1800 showed internal accumulation of E1800 in the gut and external accumulation on the respiratory siphon, anal papillae, and setae, suggesting a physical mode of toxic action. Taken together, our results suggest that E1800 has potential use as a larvicide with a novel mode of action for controlling PS and PR mosquitoes.