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Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin

Environmental contamination by nanoparticles (NPs) and drugs represents one of the most debated issues of the last years. The aquatic biome and, indirectly, human health are strongly influenced by the negative effects induced by the widespread presence of pharmaceutical products in wastewater, mainl...

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Autores principales: Mottola, Filomena, Iovine, Concetta, Santonastaso, Marianna, Carfora, Vincenzo, Pacifico, Severina, Rocco, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954801/
https://www.ncbi.nlm.nih.gov/pubmed/35324757
http://dx.doi.org/10.3390/toxics10030132
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author Mottola, Filomena
Iovine, Concetta
Santonastaso, Marianna
Carfora, Vincenzo
Pacifico, Severina
Rocco, Lucia
author_facet Mottola, Filomena
Iovine, Concetta
Santonastaso, Marianna
Carfora, Vincenzo
Pacifico, Severina
Rocco, Lucia
author_sort Mottola, Filomena
collection PubMed
description Environmental contamination by nanoparticles (NPs) and drugs represents one of the most debated issues of the last years. The aquatic biome and, indirectly, human health are strongly influenced by the negative effects induced by the widespread presence of pharmaceutical products in wastewater, mainly due to the massive use of antibiotics and inefficient treatment of the waters. The present study aimed to evaluate the harmful consequences due to exposure to antibiotics and NPs, alone and in combination, in the aquatic environment. By exploiting some of their peculiar characteristics, such as small size and ability to bind different types of substances, NPs can carry drugs into the body, showing potential genotoxic effects. The research was conducted on zebrafish (Danio rerio) exposed in vivo to lincomycin (100 mg/L) and titanium dioxide nanoparticles (TiO(2) NPs) (10 µg/L) for 7 and 14 exposure days. The effects on zebrafish were evaluated in terms of cell viability, DNA fragmentation, and genomic template stability (GTS%) investigated using Trypan blue staining, TUNEL assay, and the random amplification of polymorphic DNA PCR (RAPD PCR) technique, respectively. Our results show that after TiO(2) NPs exposure, as well as after TiO(2) NPs and lincomycin co-exposure, the percentage of damaged DNA significantly increased and cell viability decreased. On the contrary, exposure to lincomycin alone caused only a GTS% reduction after 14 exposure days. Therefore, the results allow us to assert that genotoxic effect in target cells could be through a synergistic effect, also potentially mediated by the establishment of intermolecular interactions between lincomycin and TiO(2) NPs.
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spelling pubmed-89548012022-03-26 Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin Mottola, Filomena Iovine, Concetta Santonastaso, Marianna Carfora, Vincenzo Pacifico, Severina Rocco, Lucia Toxics Article Environmental contamination by nanoparticles (NPs) and drugs represents one of the most debated issues of the last years. The aquatic biome and, indirectly, human health are strongly influenced by the negative effects induced by the widespread presence of pharmaceutical products in wastewater, mainly due to the massive use of antibiotics and inefficient treatment of the waters. The present study aimed to evaluate the harmful consequences due to exposure to antibiotics and NPs, alone and in combination, in the aquatic environment. By exploiting some of their peculiar characteristics, such as small size and ability to bind different types of substances, NPs can carry drugs into the body, showing potential genotoxic effects. The research was conducted on zebrafish (Danio rerio) exposed in vivo to lincomycin (100 mg/L) and titanium dioxide nanoparticles (TiO(2) NPs) (10 µg/L) for 7 and 14 exposure days. The effects on zebrafish were evaluated in terms of cell viability, DNA fragmentation, and genomic template stability (GTS%) investigated using Trypan blue staining, TUNEL assay, and the random amplification of polymorphic DNA PCR (RAPD PCR) technique, respectively. Our results show that after TiO(2) NPs exposure, as well as after TiO(2) NPs and lincomycin co-exposure, the percentage of damaged DNA significantly increased and cell viability decreased. On the contrary, exposure to lincomycin alone caused only a GTS% reduction after 14 exposure days. Therefore, the results allow us to assert that genotoxic effect in target cells could be through a synergistic effect, also potentially mediated by the establishment of intermolecular interactions between lincomycin and TiO(2) NPs. MDPI 2022-03-08 /pmc/articles/PMC8954801/ /pubmed/35324757 http://dx.doi.org/10.3390/toxics10030132 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mottola, Filomena
Iovine, Concetta
Santonastaso, Marianna
Carfora, Vincenzo
Pacifico, Severina
Rocco, Lucia
Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin
title Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin
title_full Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin
title_fullStr Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin
title_full_unstemmed Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin
title_short Evaluation of Zebrafish DNA Integrity after Individual and Combined Exposure to TiO(2) Nanoparticles and Lincomycin
title_sort evaluation of zebrafish dna integrity after individual and combined exposure to tio(2) nanoparticles and lincomycin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954801/
https://www.ncbi.nlm.nih.gov/pubmed/35324757
http://dx.doi.org/10.3390/toxics10030132
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