Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes

Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly impr...

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Detalles Bibliográficos
Autores principales: Antoshkina, Irina V., Glazkova, Dina V., Urusov, Felix A., Bogoslovskaya, Elena V., Shipulin, German A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954814/
https://www.ncbi.nlm.nih.gov/pubmed/35336935
http://dx.doi.org/10.3390/v14030528
Descripción
Sumario:Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes.

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