Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes

Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly impr...

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Detalles Bibliográficos
Autores principales: Antoshkina, Irina V., Glazkova, Dina V., Urusov, Felix A., Bogoslovskaya, Elena V., Shipulin, German A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954814/
https://www.ncbi.nlm.nih.gov/pubmed/35336935
http://dx.doi.org/10.3390/v14030528
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author Antoshkina, Irina V.
Glazkova, Dina V.
Urusov, Felix A.
Bogoslovskaya, Elena V.
Shipulin, German A.
author_facet Antoshkina, Irina V.
Glazkova, Dina V.
Urusov, Felix A.
Bogoslovskaya, Elena V.
Shipulin, German A.
author_sort Antoshkina, Irina V.
collection PubMed
description Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes.
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spelling pubmed-89548142022-03-26 Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes Antoshkina, Irina V. Glazkova, Dina V. Urusov, Felix A. Bogoslovskaya, Elena V. Shipulin, German A. Viruses Article Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes. MDPI 2022-03-04 /pmc/articles/PMC8954814/ /pubmed/35336935 http://dx.doi.org/10.3390/v14030528 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Antoshkina, Irina V.
Glazkova, Dina V.
Urusov, Felix A.
Bogoslovskaya, Elena V.
Shipulin, German A.
Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
title Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
title_full Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
title_fullStr Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
title_full_unstemmed Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
title_short Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
title_sort comparison of recombinant mva selection methods based on f13l, d4r and k1l genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8954814/
https://www.ncbi.nlm.nih.gov/pubmed/35336935
http://dx.doi.org/10.3390/v14030528
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