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Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?
Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955189/ https://www.ncbi.nlm.nih.gov/pubmed/35328683 http://dx.doi.org/10.3390/ijms23063262 |
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author | Grzanka, Małgorzata Stachurska-Skrodzka, Anna Adamiok-Ostrowska, Anna Gajda, Ewa Czarnocka, Barbara |
author_facet | Grzanka, Małgorzata Stachurska-Skrodzka, Anna Adamiok-Ostrowska, Anna Gajda, Ewa Czarnocka, Barbara |
author_sort | Grzanka, Małgorzata |
collection | PubMed |
description | Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lines (CGTH, FTC-133, 8505c, TPC-1 and BcPAP) were used for treatment of normal thyroid cells (NTHY), as well as monocytes and endothelial cells (HUVEC). EVs’ size/number were analyzed by flow cytometry and confocal microscopy. Gene expression, protein level and localization were investigated by qRT-PCR, WB and ICC/IF, respectively. Proliferation, migration and tube formation were analyzed. When compared with NTHY, CGTH and BcPAP secreted significantly more EVs. Treatment of NTHY with cancer-derived EVs changed the expression of tetraspanin genes, but did not affect proliferation and migration. Cancer-derived EVs suppressed tube formation by endothelial cells and did not affect the phagocytic index of monocytes. The number of 6 μm size fraction of cancer-derived EVs correlated negatively with the CD63 and CD81 expression in NTHY cells, as well as positively with angiogenesis in vitro. Thyroid cancer-derived EVs can affect the expression of tetraspanins in normal thyroid cells. It is possible that 6 μm EVs contribute to the regulation of NTHY gene expression and angiogenesis. |
format | Online Article Text |
id | pubmed-8955189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89551892022-03-26 Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? Grzanka, Małgorzata Stachurska-Skrodzka, Anna Adamiok-Ostrowska, Anna Gajda, Ewa Czarnocka, Barbara Int J Mol Sci Article Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lines (CGTH, FTC-133, 8505c, TPC-1 and BcPAP) were used for treatment of normal thyroid cells (NTHY), as well as monocytes and endothelial cells (HUVEC). EVs’ size/number were analyzed by flow cytometry and confocal microscopy. Gene expression, protein level and localization were investigated by qRT-PCR, WB and ICC/IF, respectively. Proliferation, migration and tube formation were analyzed. When compared with NTHY, CGTH and BcPAP secreted significantly more EVs. Treatment of NTHY with cancer-derived EVs changed the expression of tetraspanin genes, but did not affect proliferation and migration. Cancer-derived EVs suppressed tube formation by endothelial cells and did not affect the phagocytic index of monocytes. The number of 6 μm size fraction of cancer-derived EVs correlated negatively with the CD63 and CD81 expression in NTHY cells, as well as positively with angiogenesis in vitro. Thyroid cancer-derived EVs can affect the expression of tetraspanins in normal thyroid cells. It is possible that 6 μm EVs contribute to the regulation of NTHY gene expression and angiogenesis. MDPI 2022-03-17 /pmc/articles/PMC8955189/ /pubmed/35328683 http://dx.doi.org/10.3390/ijms23063262 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grzanka, Małgorzata Stachurska-Skrodzka, Anna Adamiok-Ostrowska, Anna Gajda, Ewa Czarnocka, Barbara Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? |
title | Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? |
title_full | Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? |
title_fullStr | Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? |
title_full_unstemmed | Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? |
title_short | Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors? |
title_sort | extracellular vesicles as signal carriers in malignant thyroid tumors? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955189/ https://www.ncbi.nlm.nih.gov/pubmed/35328683 http://dx.doi.org/10.3390/ijms23063262 |
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