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Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein

Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is a tick-borne zoonotic virus that causes hemorrhagic fever in humans. The CCHFV nucleoprotein (NP) is the antigen most used for serological screening of CCHFV infection in animals and humans. To gain insights into antibody epitopes on th...

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Autores principales: Lombe, Boniface Pongombo, Saito, Takeshi, Miyamoto, Hiroko, Mori-Kajihara, Akina, Kajihara, Masahiro, Saijo, Masayuki, Masumu, Justin, Hattori, Takanari, Igarashi, Manabu, Takada, Ayato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955205/
https://www.ncbi.nlm.nih.gov/pubmed/35336951
http://dx.doi.org/10.3390/v14030544
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author Lombe, Boniface Pongombo
Saito, Takeshi
Miyamoto, Hiroko
Mori-Kajihara, Akina
Kajihara, Masahiro
Saijo, Masayuki
Masumu, Justin
Hattori, Takanari
Igarashi, Manabu
Takada, Ayato
author_facet Lombe, Boniface Pongombo
Saito, Takeshi
Miyamoto, Hiroko
Mori-Kajihara, Akina
Kajihara, Masahiro
Saijo, Masayuki
Masumu, Justin
Hattori, Takanari
Igarashi, Manabu
Takada, Ayato
author_sort Lombe, Boniface Pongombo
collection PubMed
description Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is a tick-borne zoonotic virus that causes hemorrhagic fever in humans. The CCHFV nucleoprotein (NP) is the antigen most used for serological screening of CCHFV infection in animals and humans. To gain insights into antibody epitopes on the NP molecule, we produced recombinant chimeric NPs between CCHFV and Nairobi sheep disease virus (NSDV), which is another nairovirus, and tested rabbit and mouse antisera/immune ascites, anti-NP monoclonal antibodies, and CCHFV-infected animal/human sera for their reactivities to the NP antigens. We found that the amino acids at positions 161–320 might include dominant epitopes recognized by anti-CCHFV IgG antibodies, whereas cross-reactivity between anti-CCHFV and anti-NSDV antibodies was limited. Their binding capacities were further tested using a series of synthetic peptides whose sequences were derived from CCHFV NP. IgG antibodies in CCHFV-infected monkeys and patients were reactive to some of the synthetic peptide antigens (e.g., amino acid residues at positions 131–150 and 211–230). Only a few peptides were recognized by IgG antibodies in the anti-NSDV serum. These results provide useful information to improve NP-based antibody detection assays as well as antigen detection tests relying on anti-NP monoclonal antibodies.
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spelling pubmed-89552052022-03-26 Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein Lombe, Boniface Pongombo Saito, Takeshi Miyamoto, Hiroko Mori-Kajihara, Akina Kajihara, Masahiro Saijo, Masayuki Masumu, Justin Hattori, Takanari Igarashi, Manabu Takada, Ayato Viruses Article Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is a tick-borne zoonotic virus that causes hemorrhagic fever in humans. The CCHFV nucleoprotein (NP) is the antigen most used for serological screening of CCHFV infection in animals and humans. To gain insights into antibody epitopes on the NP molecule, we produced recombinant chimeric NPs between CCHFV and Nairobi sheep disease virus (NSDV), which is another nairovirus, and tested rabbit and mouse antisera/immune ascites, anti-NP monoclonal antibodies, and CCHFV-infected animal/human sera for their reactivities to the NP antigens. We found that the amino acids at positions 161–320 might include dominant epitopes recognized by anti-CCHFV IgG antibodies, whereas cross-reactivity between anti-CCHFV and anti-NSDV antibodies was limited. Their binding capacities were further tested using a series of synthetic peptides whose sequences were derived from CCHFV NP. IgG antibodies in CCHFV-infected monkeys and patients were reactive to some of the synthetic peptide antigens (e.g., amino acid residues at positions 131–150 and 211–230). Only a few peptides were recognized by IgG antibodies in the anti-NSDV serum. These results provide useful information to improve NP-based antibody detection assays as well as antigen detection tests relying on anti-NP monoclonal antibodies. MDPI 2022-03-06 /pmc/articles/PMC8955205/ /pubmed/35336951 http://dx.doi.org/10.3390/v14030544 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lombe, Boniface Pongombo
Saito, Takeshi
Miyamoto, Hiroko
Mori-Kajihara, Akina
Kajihara, Masahiro
Saijo, Masayuki
Masumu, Justin
Hattori, Takanari
Igarashi, Manabu
Takada, Ayato
Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
title Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
title_full Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
title_fullStr Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
title_full_unstemmed Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
title_short Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
title_sort mapping of antibody epitopes on the crimean-congo hemorrhagic fever virus nucleoprotein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955205/
https://www.ncbi.nlm.nih.gov/pubmed/35336951
http://dx.doi.org/10.3390/v14030544
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