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Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies

The Mediterranean fruit fly (medfly), Ceratitis capitata, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA profile of medfly. B...

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Autores principales: Hernández-Pelegrín, Luis, Llopis-Giménez, Ángel, Crava, Cristina Maria, Ortego, Félix, Hernández-Crespo, Pedro, Ros, Vera I. D., Herrero, Salvador
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955247/
https://www.ncbi.nlm.nih.gov/pubmed/35337030
http://dx.doi.org/10.3390/v14030623
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author Hernández-Pelegrín, Luis
Llopis-Giménez, Ángel
Crava, Cristina Maria
Ortego, Félix
Hernández-Crespo, Pedro
Ros, Vera I. D.
Herrero, Salvador
author_facet Hernández-Pelegrín, Luis
Llopis-Giménez, Ángel
Crava, Cristina Maria
Ortego, Félix
Hernández-Crespo, Pedro
Ros, Vera I. D.
Herrero, Salvador
author_sort Hernández-Pelegrín, Luis
collection PubMed
description The Mediterranean fruit fly (medfly), Ceratitis capitata, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA profile of medfly. By means of transcriptome mining, we expanded the medfly RNA virome to 13 viruses, including two novel positive ssRNA viruses and the first two novel dsRNA viruses reported for medfly. Our analysis across multiple laboratory-reared and field-collected medfly samples showed the presence of a core RNA virome comprised of Ceratitis capitata iflavirus 2 and Ceratitis capitata negev-like virus 1. Furthermore, field-collected flies showed a higher viral diversity in comparison to the laboratory-reared flies. Based on the small RNA sequencing, we detected small interfering RNAs mapping to all the viruses present in each sample, except for Ceratitis capitata nora virus. Although the identified RNA viruses do not cause obvious symptoms in medflies, the outcome of their interaction may still influence the medfly’s fitness and ecology, becoming either a risk or an opportunity for mass-rearing and SIT applications.
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spelling pubmed-89552472022-03-26 Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies Hernández-Pelegrín, Luis Llopis-Giménez, Ángel Crava, Cristina Maria Ortego, Félix Hernández-Crespo, Pedro Ros, Vera I. D. Herrero, Salvador Viruses Article The Mediterranean fruit fly (medfly), Ceratitis capitata, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA profile of medfly. By means of transcriptome mining, we expanded the medfly RNA virome to 13 viruses, including two novel positive ssRNA viruses and the first two novel dsRNA viruses reported for medfly. Our analysis across multiple laboratory-reared and field-collected medfly samples showed the presence of a core RNA virome comprised of Ceratitis capitata iflavirus 2 and Ceratitis capitata negev-like virus 1. Furthermore, field-collected flies showed a higher viral diversity in comparison to the laboratory-reared flies. Based on the small RNA sequencing, we detected small interfering RNAs mapping to all the viruses present in each sample, except for Ceratitis capitata nora virus. Although the identified RNA viruses do not cause obvious symptoms in medflies, the outcome of their interaction may still influence the medfly’s fitness and ecology, becoming either a risk or an opportunity for mass-rearing and SIT applications. MDPI 2022-03-17 /pmc/articles/PMC8955247/ /pubmed/35337030 http://dx.doi.org/10.3390/v14030623 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Pelegrín, Luis
Llopis-Giménez, Ángel
Crava, Cristina Maria
Ortego, Félix
Hernández-Crespo, Pedro
Ros, Vera I. D.
Herrero, Salvador
Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
title Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
title_full Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
title_fullStr Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
title_full_unstemmed Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
title_short Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies
title_sort expanding the medfly virome: viral diversity, prevalence, and srna profiling in mass-reared and field-derived medflies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955247/
https://www.ncbi.nlm.nih.gov/pubmed/35337030
http://dx.doi.org/10.3390/v14030623
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