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Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties

Nickel nanoparticles (NPs) are used for soil remediation and wastewater treatment due to their high adsorption capacity against complex organic pollutants. However, despite the growing use of nickel NPs, their toxicological towards environmental bacteria have not been sufficiently studied. Actinobac...

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Autores principales: Kuyukina, Maria S., Glebov, Grigorii G., Ivshina, Irena B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955278/
https://www.ncbi.nlm.nih.gov/pubmed/35335763
http://dx.doi.org/10.3390/nano12060951
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author Kuyukina, Maria S.
Glebov, Grigorii G.
Ivshina, Irena B.
author_facet Kuyukina, Maria S.
Glebov, Grigorii G.
Ivshina, Irena B.
author_sort Kuyukina, Maria S.
collection PubMed
description Nickel nanoparticles (NPs) are used for soil remediation and wastewater treatment due to their high adsorption capacity against complex organic pollutants. However, despite the growing use of nickel NPs, their toxicological towards environmental bacteria have not been sufficiently studied. Actinobacteria of the genus Rhodococcus are valuable bioremediation agents degrading a range of harmful and recalcitrant chemicals. Both positive and negative effects of metal ions and NPs on the biodegradation of organic pollutants by Rhodococcus were revealed, however, the mechanisms of such interactions, in addition to direct toxic effects, remain unclear. In the present work, the influence of nickel NPs on the viability, surface topology and nanomechanical properties of Rhodococcus cells have been studied. Bacterial adaptations to high (up to 1.0 g/L) concentrations of nickel NPs during prolonged (24 and 48 h) exposure were detected using combined confocal laser scanning and atomic force microscopy. Incubation with nickel NPs resulted in a 1.25–1.5-fold increase in the relative surface area and roughness, changes in cellular charge and adhesion characteristics, as well as a 2–8-fold decrease in the Young’s modulus of Rhodococcus ruber IEGM 231 cells. Presumably, the treatment of rhodococcal cells with sublethal concentrations (0.01–0.1 g/L) of nickel NPs facilitates the colonization of surfaces, which is important in the production of immobilized biocatalysts based on whole bacterial cells adsorbed on solid carriers. Based on the data obtained, cell surface functionalizing with NPs is possible to enhance adhesive and catalytic properties of bacteria suitable for environmental applications.
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spelling pubmed-89552782022-03-26 Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties Kuyukina, Maria S. Glebov, Grigorii G. Ivshina, Irena B. Nanomaterials (Basel) Article Nickel nanoparticles (NPs) are used for soil remediation and wastewater treatment due to their high adsorption capacity against complex organic pollutants. However, despite the growing use of nickel NPs, their toxicological towards environmental bacteria have not been sufficiently studied. Actinobacteria of the genus Rhodococcus are valuable bioremediation agents degrading a range of harmful and recalcitrant chemicals. Both positive and negative effects of metal ions and NPs on the biodegradation of organic pollutants by Rhodococcus were revealed, however, the mechanisms of such interactions, in addition to direct toxic effects, remain unclear. In the present work, the influence of nickel NPs on the viability, surface topology and nanomechanical properties of Rhodococcus cells have been studied. Bacterial adaptations to high (up to 1.0 g/L) concentrations of nickel NPs during prolonged (24 and 48 h) exposure were detected using combined confocal laser scanning and atomic force microscopy. Incubation with nickel NPs resulted in a 1.25–1.5-fold increase in the relative surface area and roughness, changes in cellular charge and adhesion characteristics, as well as a 2–8-fold decrease in the Young’s modulus of Rhodococcus ruber IEGM 231 cells. Presumably, the treatment of rhodococcal cells with sublethal concentrations (0.01–0.1 g/L) of nickel NPs facilitates the colonization of surfaces, which is important in the production of immobilized biocatalysts based on whole bacterial cells adsorbed on solid carriers. Based on the data obtained, cell surface functionalizing with NPs is possible to enhance adhesive and catalytic properties of bacteria suitable for environmental applications. MDPI 2022-03-14 /pmc/articles/PMC8955278/ /pubmed/35335763 http://dx.doi.org/10.3390/nano12060951 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuyukina, Maria S.
Glebov, Grigorii G.
Ivshina, Irena B.
Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties
title Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties
title_full Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties
title_fullStr Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties
title_full_unstemmed Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties
title_short Effects of Nickel Nanoparticles on Rhodococcus Cell Surface Morphology and Nanomechanical Properties
title_sort effects of nickel nanoparticles on rhodococcus cell surface morphology and nanomechanical properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955278/
https://www.ncbi.nlm.nih.gov/pubmed/35335763
http://dx.doi.org/10.3390/nano12060951
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