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Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows

This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 10(6.0) EID(50) of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation...

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Autores principales: Verma, Asha Kumari, Kumar, Manoj, Murugkar, Harshad V., Nagarajan, Shanmugasundaram, Tosh, Chakradhar, Namdeo, Pushpendra, Singh, Rupal, Mishra, Suman, Kombiah, Subbiah, Dhanapal, Senthilkumar, Singh, Vijendra Pal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955285/
https://www.ncbi.nlm.nih.gov/pubmed/35335628
http://dx.doi.org/10.3390/pathogens11030304
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author Verma, Asha Kumari
Kumar, Manoj
Murugkar, Harshad V.
Nagarajan, Shanmugasundaram
Tosh, Chakradhar
Namdeo, Pushpendra
Singh, Rupal
Mishra, Suman
Kombiah, Subbiah
Dhanapal, Senthilkumar
Singh, Vijendra Pal
author_facet Verma, Asha Kumari
Kumar, Manoj
Murugkar, Harshad V.
Nagarajan, Shanmugasundaram
Tosh, Chakradhar
Namdeo, Pushpendra
Singh, Rupal
Mishra, Suman
Kombiah, Subbiah
Dhanapal, Senthilkumar
Singh, Vijendra Pal
author_sort Verma, Asha Kumari
collection PubMed
description This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 10(6.0) EID(50) of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation six naïve crows were co-housed with infected crows. Crows were observed for 14 days for any overt signs of illness. Oropharyngeal and cloacal swabs were collected up to 14 days to assess virus excretion. No apparent clinical signs were observed in either infected or in-contact crows. Virus excretion was observed only in infected birds up to 9 days post-infection (dpi) through both oropharyngeal and cloacal routes. All six infected crows seroconverted to H9N2 virus at 14 dpi, whereas all in-contact crows remained negative to H9N2 virus antibodies. No virus could be isolated from tissues viz., lung, liver, kidney, pancreas, small intestine and large intestine. Although crows became infected with the H9N2 virus, transmission of the virus was inefficient to the in-contact group. However, virus excretion through oral and cloacal swabs from infected crows suggests a potential threat for inter-species transmission, including humans. Crows, being a common synanthrope species, might have some role in influenza virus transmission to poultry and humans, which needs to be explored further.
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spelling pubmed-89552852022-03-26 Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows Verma, Asha Kumari Kumar, Manoj Murugkar, Harshad V. Nagarajan, Shanmugasundaram Tosh, Chakradhar Namdeo, Pushpendra Singh, Rupal Mishra, Suman Kombiah, Subbiah Dhanapal, Senthilkumar Singh, Vijendra Pal Pathogens Communication This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 10(6.0) EID(50) of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation six naïve crows were co-housed with infected crows. Crows were observed for 14 days for any overt signs of illness. Oropharyngeal and cloacal swabs were collected up to 14 days to assess virus excretion. No apparent clinical signs were observed in either infected or in-contact crows. Virus excretion was observed only in infected birds up to 9 days post-infection (dpi) through both oropharyngeal and cloacal routes. All six infected crows seroconverted to H9N2 virus at 14 dpi, whereas all in-contact crows remained negative to H9N2 virus antibodies. No virus could be isolated from tissues viz., lung, liver, kidney, pancreas, small intestine and large intestine. Although crows became infected with the H9N2 virus, transmission of the virus was inefficient to the in-contact group. However, virus excretion through oral and cloacal swabs from infected crows suggests a potential threat for inter-species transmission, including humans. Crows, being a common synanthrope species, might have some role in influenza virus transmission to poultry and humans, which needs to be explored further. MDPI 2022-02-28 /pmc/articles/PMC8955285/ /pubmed/35335628 http://dx.doi.org/10.3390/pathogens11030304 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Verma, Asha Kumari
Kumar, Manoj
Murugkar, Harshad V.
Nagarajan, Shanmugasundaram
Tosh, Chakradhar
Namdeo, Pushpendra
Singh, Rupal
Mishra, Suman
Kombiah, Subbiah
Dhanapal, Senthilkumar
Singh, Vijendra Pal
Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
title Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
title_full Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
title_fullStr Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
title_full_unstemmed Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
title_short Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
title_sort experimental infection and in-contact transmission of h9n2 avian influenza virus in crows
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955285/
https://www.ncbi.nlm.nih.gov/pubmed/35335628
http://dx.doi.org/10.3390/pathogens11030304
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