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Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 10(6.0) EID(50) of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955285/ https://www.ncbi.nlm.nih.gov/pubmed/35335628 http://dx.doi.org/10.3390/pathogens11030304 |
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author | Verma, Asha Kumari Kumar, Manoj Murugkar, Harshad V. Nagarajan, Shanmugasundaram Tosh, Chakradhar Namdeo, Pushpendra Singh, Rupal Mishra, Suman Kombiah, Subbiah Dhanapal, Senthilkumar Singh, Vijendra Pal |
author_facet | Verma, Asha Kumari Kumar, Manoj Murugkar, Harshad V. Nagarajan, Shanmugasundaram Tosh, Chakradhar Namdeo, Pushpendra Singh, Rupal Mishra, Suman Kombiah, Subbiah Dhanapal, Senthilkumar Singh, Vijendra Pal |
author_sort | Verma, Asha Kumari |
collection | PubMed |
description | This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 10(6.0) EID(50) of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation six naïve crows were co-housed with infected crows. Crows were observed for 14 days for any overt signs of illness. Oropharyngeal and cloacal swabs were collected up to 14 days to assess virus excretion. No apparent clinical signs were observed in either infected or in-contact crows. Virus excretion was observed only in infected birds up to 9 days post-infection (dpi) through both oropharyngeal and cloacal routes. All six infected crows seroconverted to H9N2 virus at 14 dpi, whereas all in-contact crows remained negative to H9N2 virus antibodies. No virus could be isolated from tissues viz., lung, liver, kidney, pancreas, small intestine and large intestine. Although crows became infected with the H9N2 virus, transmission of the virus was inefficient to the in-contact group. However, virus excretion through oral and cloacal swabs from infected crows suggests a potential threat for inter-species transmission, including humans. Crows, being a common synanthrope species, might have some role in influenza virus transmission to poultry and humans, which needs to be explored further. |
format | Online Article Text |
id | pubmed-8955285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89552852022-03-26 Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows Verma, Asha Kumari Kumar, Manoj Murugkar, Harshad V. Nagarajan, Shanmugasundaram Tosh, Chakradhar Namdeo, Pushpendra Singh, Rupal Mishra, Suman Kombiah, Subbiah Dhanapal, Senthilkumar Singh, Vijendra Pal Pathogens Communication This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 10(6.0) EID(50) of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation six naïve crows were co-housed with infected crows. Crows were observed for 14 days for any overt signs of illness. Oropharyngeal and cloacal swabs were collected up to 14 days to assess virus excretion. No apparent clinical signs were observed in either infected or in-contact crows. Virus excretion was observed only in infected birds up to 9 days post-infection (dpi) through both oropharyngeal and cloacal routes. All six infected crows seroconverted to H9N2 virus at 14 dpi, whereas all in-contact crows remained negative to H9N2 virus antibodies. No virus could be isolated from tissues viz., lung, liver, kidney, pancreas, small intestine and large intestine. Although crows became infected with the H9N2 virus, transmission of the virus was inefficient to the in-contact group. However, virus excretion through oral and cloacal swabs from infected crows suggests a potential threat for inter-species transmission, including humans. Crows, being a common synanthrope species, might have some role in influenza virus transmission to poultry and humans, which needs to be explored further. MDPI 2022-02-28 /pmc/articles/PMC8955285/ /pubmed/35335628 http://dx.doi.org/10.3390/pathogens11030304 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Verma, Asha Kumari Kumar, Manoj Murugkar, Harshad V. Nagarajan, Shanmugasundaram Tosh, Chakradhar Namdeo, Pushpendra Singh, Rupal Mishra, Suman Kombiah, Subbiah Dhanapal, Senthilkumar Singh, Vijendra Pal Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows |
title | Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows |
title_full | Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows |
title_fullStr | Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows |
title_full_unstemmed | Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows |
title_short | Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows |
title_sort | experimental infection and in-contact transmission of h9n2 avian influenza virus in crows |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955285/ https://www.ncbi.nlm.nih.gov/pubmed/35335628 http://dx.doi.org/10.3390/pathogens11030304 |
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