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Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L.
Calotropis gigantea is often found in mining areas with heavy metal pollution. However, little is known about the physiological and molecular response mechanism of C. gigantea to Cd stress. In the present study, Cd tolerance characteristic of C. gigantea and the potential mechanisms were explored. S...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955323/ https://www.ncbi.nlm.nih.gov/pubmed/35328749 http://dx.doi.org/10.3390/ijms23063329 |
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author | Yang, Jingya Li, Lingxiong Zhang, Xiong Wu, Shibo Han, Xiaohui Li, Xiong Xu, Jianchu |
author_facet | Yang, Jingya Li, Lingxiong Zhang, Xiong Wu, Shibo Han, Xiaohui Li, Xiong Xu, Jianchu |
author_sort | Yang, Jingya |
collection | PubMed |
description | Calotropis gigantea is often found in mining areas with heavy metal pollution. However, little is known about the physiological and molecular response mechanism of C. gigantea to Cd stress. In the present study, Cd tolerance characteristic of C. gigantea and the potential mechanisms were explored. Seed germination test results showed that C. gigantea had a certain Cd tolerance capacity. Biochemical and transcriptomic analysis indicated that the roots and leaves of C. gigantea had different responses to early Cd stress. A total of 176 and 1618 DEGs were identified in the roots and leaves of C. gigantea treated with Cd compared to the control samples, respectively. Results indicated that oxidative stress was mainly initiated in the roots of C. gigantea, whereas the leaves activated several Cd detoxification processes to cope with Cd, including the upregulation of genes involved in Cd transport (i.e., absorption, efflux, or compartmentalization), cell wall remodeling, antioxidant system, and chelation. This study provides preliminary information to understand how C. gigantea respond to Cd stress, which is useful for evaluating the potential of C. gigantea in the remediation of Cd-contaminated soils. |
format | Online Article Text |
id | pubmed-8955323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89553232022-03-26 Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. Yang, Jingya Li, Lingxiong Zhang, Xiong Wu, Shibo Han, Xiaohui Li, Xiong Xu, Jianchu Int J Mol Sci Article Calotropis gigantea is often found in mining areas with heavy metal pollution. However, little is known about the physiological and molecular response mechanism of C. gigantea to Cd stress. In the present study, Cd tolerance characteristic of C. gigantea and the potential mechanisms were explored. Seed germination test results showed that C. gigantea had a certain Cd tolerance capacity. Biochemical and transcriptomic analysis indicated that the roots and leaves of C. gigantea had different responses to early Cd stress. A total of 176 and 1618 DEGs were identified in the roots and leaves of C. gigantea treated with Cd compared to the control samples, respectively. Results indicated that oxidative stress was mainly initiated in the roots of C. gigantea, whereas the leaves activated several Cd detoxification processes to cope with Cd, including the upregulation of genes involved in Cd transport (i.e., absorption, efflux, or compartmentalization), cell wall remodeling, antioxidant system, and chelation. This study provides preliminary information to understand how C. gigantea respond to Cd stress, which is useful for evaluating the potential of C. gigantea in the remediation of Cd-contaminated soils. MDPI 2022-03-19 /pmc/articles/PMC8955323/ /pubmed/35328749 http://dx.doi.org/10.3390/ijms23063329 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Jingya Li, Lingxiong Zhang, Xiong Wu, Shibo Han, Xiaohui Li, Xiong Xu, Jianchu Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. |
title | Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. |
title_full | Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. |
title_fullStr | Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. |
title_full_unstemmed | Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. |
title_short | Comparative Transcriptomics Analysis of Roots and Leaves under Cd Stress in Calotropis gigantea L. |
title_sort | comparative transcriptomics analysis of roots and leaves under cd stress in calotropis gigantea l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955323/ https://www.ncbi.nlm.nih.gov/pubmed/35328749 http://dx.doi.org/10.3390/ijms23063329 |
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