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Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.

Cytochrome P450s (P450ome) constitute an extended superfamily group of heme-thiolate enzymes identified in all biological domains. P450omes play a critical role in the oxidation of steroids and fatty acids, xenobiotic degradation of hydrophobic compounds, biosynthesis of hormones, and primary and se...

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Autores principales: Dauda, Wadzani Palnam, Abraham, Peter, Glen, Elkanah, Adetunji, Charles Oluwaseun, Ghazanfar, Shakira, Ali, Shafaqat, Al-Zahrani, Majid, Azameti, Mawuli Kwamla, Alao, Sheik Emmanuel Laykay, Zarafi, Afiniki Bawa, Abraham, Maryam Peter, Musa, Hannatu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955511/
https://www.ncbi.nlm.nih.gov/pubmed/35330202
http://dx.doi.org/10.3390/life12030451
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author Dauda, Wadzani Palnam
Abraham, Peter
Glen, Elkanah
Adetunji, Charles Oluwaseun
Ghazanfar, Shakira
Ali, Shafaqat
Al-Zahrani, Majid
Azameti, Mawuli Kwamla
Alao, Sheik Emmanuel Laykay
Zarafi, Afiniki Bawa
Abraham, Maryam Peter
Musa, Hannatu
author_facet Dauda, Wadzani Palnam
Abraham, Peter
Glen, Elkanah
Adetunji, Charles Oluwaseun
Ghazanfar, Shakira
Ali, Shafaqat
Al-Zahrani, Majid
Azameti, Mawuli Kwamla
Alao, Sheik Emmanuel Laykay
Zarafi, Afiniki Bawa
Abraham, Maryam Peter
Musa, Hannatu
author_sort Dauda, Wadzani Palnam
collection PubMed
description Cytochrome P450s (P450ome) constitute an extended superfamily group of heme-thiolate enzymes identified in all biological domains. P450omes play a critical role in the oxidation of steroids and fatty acids, xenobiotic degradation of hydrophobic compounds, biosynthesis of hormones, and primary and secondary metabolism in organisms. Aspergillus species are among the most economically important fungal organisms in human medicine, industry, and agriculture worldwide. Exploring insight on the genome-wide annotations of cytochrome P450s in Aspergillus species is necessary for their biosynthetic applications. In this present study, we report the identification of 306 cytochrome P450s and their robust profiling in eight notable Aspergillus species (A. carbonarius, A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, and A. terreus). Based on the evolutionary relationship, the Aspergillus P450s families clustered into 15 clades, with clades V, I, and XIII recording higher percentages (17.3%, 15.00%, and 14.71%, respectively) of Cyp families. Cyps were classified into 120 families 64 clans, and their putative functions were also elucidated. P450s were predicted to be located in 13 subcellular components, but the endoplasm reticulum was the dominant location across the eight Aspergillus species. Cyps genes of Aspergillus species were associated with seven secondary metabolism-related gene clusters. Elucidating the genome-wide annotations of P450s enzymes in Aspergillus species will form vital potential biotechnological tools that could be harnessed for industrial, pharmaceutical, and agricultural use.
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spelling pubmed-89555112022-03-26 Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp. Dauda, Wadzani Palnam Abraham, Peter Glen, Elkanah Adetunji, Charles Oluwaseun Ghazanfar, Shakira Ali, Shafaqat Al-Zahrani, Majid Azameti, Mawuli Kwamla Alao, Sheik Emmanuel Laykay Zarafi, Afiniki Bawa Abraham, Maryam Peter Musa, Hannatu Life (Basel) Article Cytochrome P450s (P450ome) constitute an extended superfamily group of heme-thiolate enzymes identified in all biological domains. P450omes play a critical role in the oxidation of steroids and fatty acids, xenobiotic degradation of hydrophobic compounds, biosynthesis of hormones, and primary and secondary metabolism in organisms. Aspergillus species are among the most economically important fungal organisms in human medicine, industry, and agriculture worldwide. Exploring insight on the genome-wide annotations of cytochrome P450s in Aspergillus species is necessary for their biosynthetic applications. In this present study, we report the identification of 306 cytochrome P450s and their robust profiling in eight notable Aspergillus species (A. carbonarius, A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, and A. terreus). Based on the evolutionary relationship, the Aspergillus P450s families clustered into 15 clades, with clades V, I, and XIII recording higher percentages (17.3%, 15.00%, and 14.71%, respectively) of Cyp families. Cyps were classified into 120 families 64 clans, and their putative functions were also elucidated. P450s were predicted to be located in 13 subcellular components, but the endoplasm reticulum was the dominant location across the eight Aspergillus species. Cyps genes of Aspergillus species were associated with seven secondary metabolism-related gene clusters. Elucidating the genome-wide annotations of P450s enzymes in Aspergillus species will form vital potential biotechnological tools that could be harnessed for industrial, pharmaceutical, and agricultural use. MDPI 2022-03-18 /pmc/articles/PMC8955511/ /pubmed/35330202 http://dx.doi.org/10.3390/life12030451 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dauda, Wadzani Palnam
Abraham, Peter
Glen, Elkanah
Adetunji, Charles Oluwaseun
Ghazanfar, Shakira
Ali, Shafaqat
Al-Zahrani, Majid
Azameti, Mawuli Kwamla
Alao, Sheik Emmanuel Laykay
Zarafi, Afiniki Bawa
Abraham, Maryam Peter
Musa, Hannatu
Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
title Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
title_full Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
title_fullStr Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
title_full_unstemmed Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
title_short Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp.
title_sort robust profiling of cytochrome p450s (p450ome) in notable aspergillus spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955511/
https://www.ncbi.nlm.nih.gov/pubmed/35330202
http://dx.doi.org/10.3390/life12030451
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