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Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study

The surface of titanium (Ti) dental implants must be modified to improve their applicability, owing to the biological inertness of Ti. This study aims to use sandblasting as a pretreatment method and prepare a hydroxyapatite (HA) coating on Ti to improve its biocompatibility and induce bone bonding...

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Autores principales: Ma, Lin, Li, Min, Komasa, Satoshi, Yan, Sifan, Yang, Yuanyuan, Nishizaki, Mariko, Chen, Liji, Zeng, Yuhao, Wang, Xin, Yamamoto, Ei, Hontsu, Shigeki, Hashimoto, Yoshiya, Okazaki, Joji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955651/
https://www.ncbi.nlm.nih.gov/pubmed/35329758
http://dx.doi.org/10.3390/ma15062306
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author Ma, Lin
Li, Min
Komasa, Satoshi
Yan, Sifan
Yang, Yuanyuan
Nishizaki, Mariko
Chen, Liji
Zeng, Yuhao
Wang, Xin
Yamamoto, Ei
Hontsu, Shigeki
Hashimoto, Yoshiya
Okazaki, Joji
author_facet Ma, Lin
Li, Min
Komasa, Satoshi
Yan, Sifan
Yang, Yuanyuan
Nishizaki, Mariko
Chen, Liji
Zeng, Yuhao
Wang, Xin
Yamamoto, Ei
Hontsu, Shigeki
Hashimoto, Yoshiya
Okazaki, Joji
author_sort Ma, Lin
collection PubMed
description The surface of titanium (Ti) dental implants must be modified to improve their applicability, owing to the biological inertness of Ti. This study aims to use sandblasting as a pretreatment method and prepare a hydroxyapatite (HA) coating on Ti to improve its biocompatibility and induce bone bonding and osteogenesis. In this paper, sandblasted Ti discs were coated with α-tricalcium phosphate (α-TCP) via Er:YAG pulsed laser deposition (Er:YAG-PLD). An HA coating was then obtained via the hydrothermal treatment of the discs at 90 °C for 10 h. The surface characteristics of the samples were evaluated by SEM, SPM, XPS, XRD, FTIR, and tensile tests. Rat bone marrow mesenchymal stem cells were seeded on the HA-coated discs to determine cellular responses in vitro. The surface characterization results indicated the successful transformation of the HA coating with a nanorod-like morphology, and its surface roughness increased. In vitro experiments revealed increased cell attachment on the HA-coated discs, as did the cell morphology of fluorescence staining and SEM analysis; in contrast, there was no increase in cell proliferation. This study confirms that Er:YAG-PLD could be used as an implant surface-modification technique to prepare HA coatings with a nanorod-like morphology on Ti discs.
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spelling pubmed-89556512022-03-26 Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study Ma, Lin Li, Min Komasa, Satoshi Yan, Sifan Yang, Yuanyuan Nishizaki, Mariko Chen, Liji Zeng, Yuhao Wang, Xin Yamamoto, Ei Hontsu, Shigeki Hashimoto, Yoshiya Okazaki, Joji Materials (Basel) Article The surface of titanium (Ti) dental implants must be modified to improve their applicability, owing to the biological inertness of Ti. This study aims to use sandblasting as a pretreatment method and prepare a hydroxyapatite (HA) coating on Ti to improve its biocompatibility and induce bone bonding and osteogenesis. In this paper, sandblasted Ti discs were coated with α-tricalcium phosphate (α-TCP) via Er:YAG pulsed laser deposition (Er:YAG-PLD). An HA coating was then obtained via the hydrothermal treatment of the discs at 90 °C for 10 h. The surface characteristics of the samples were evaluated by SEM, SPM, XPS, XRD, FTIR, and tensile tests. Rat bone marrow mesenchymal stem cells were seeded on the HA-coated discs to determine cellular responses in vitro. The surface characterization results indicated the successful transformation of the HA coating with a nanorod-like morphology, and its surface roughness increased. In vitro experiments revealed increased cell attachment on the HA-coated discs, as did the cell morphology of fluorescence staining and SEM analysis; in contrast, there was no increase in cell proliferation. This study confirms that Er:YAG-PLD could be used as an implant surface-modification technique to prepare HA coatings with a nanorod-like morphology on Ti discs. MDPI 2022-03-20 /pmc/articles/PMC8955651/ /pubmed/35329758 http://dx.doi.org/10.3390/ma15062306 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Lin
Li, Min
Komasa, Satoshi
Yan, Sifan
Yang, Yuanyuan
Nishizaki, Mariko
Chen, Liji
Zeng, Yuhao
Wang, Xin
Yamamoto, Ei
Hontsu, Shigeki
Hashimoto, Yoshiya
Okazaki, Joji
Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study
title Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study
title_full Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study
title_fullStr Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study
title_full_unstemmed Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study
title_short Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study
title_sort characterization of hydroxyapatite film obtained by er:yag pulsed laser deposition on sandblasted titanium: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955651/
https://www.ncbi.nlm.nih.gov/pubmed/35329758
http://dx.doi.org/10.3390/ma15062306
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