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Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place betwe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955682/ https://www.ncbi.nlm.nih.gov/pubmed/35328777 http://dx.doi.org/10.3390/ijms23063355 |
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author | Núñez-Carro, Carmen Blanco-Blanco, Margarita Montoya, Tatiana Villagrán-Andrade, Karla M. Hermida-Gómez, Tamara Blanco, Francisco J. de Andrés, María C. |
author_facet | Núñez-Carro, Carmen Blanco-Blanco, Margarita Montoya, Tatiana Villagrán-Andrade, Karla M. Hermida-Gómez, Tamara Blanco, Francisco J. de Andrés, María C. |
author_sort | Núñez-Carro, Carmen |
collection | PubMed |
description | Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. OA chondrocytes enhance the production of interleukin 1β (IL-1β) and interleukin 8 (IL-8), which are epigenetically regulated. These cytokines upregulate the synthesis of matrix metalloproteinases (MMPs) and aggrecanases, which promote the extracellular matrix (ECM) destruction. This motivates the study of histone PTMs to investigate the epigenetic regulation of proinflammatory molecules, but the absence of specific protocols to extract histones from human articular cartilage has complicated this task. The lack of effective methods can be explained by the structural complexity and low cellularity of this tissue, which are responsible for the biomechanical properties that allow the movement of the joint but also complicate histone isolation. Here, we provide a histone extraction procedure specifically adapted for cryopreserved human articular cartilage that can be useful to understand epigenetic regulation in OA and accelerate the search for novel strategies. |
format | Online Article Text |
id | pubmed-8955682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89556822022-03-26 Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis Núñez-Carro, Carmen Blanco-Blanco, Margarita Montoya, Tatiana Villagrán-Andrade, Karla M. Hermida-Gómez, Tamara Blanco, Francisco J. de Andrés, María C. Int J Mol Sci Article Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. OA chondrocytes enhance the production of interleukin 1β (IL-1β) and interleukin 8 (IL-8), which are epigenetically regulated. These cytokines upregulate the synthesis of matrix metalloproteinases (MMPs) and aggrecanases, which promote the extracellular matrix (ECM) destruction. This motivates the study of histone PTMs to investigate the epigenetic regulation of proinflammatory molecules, but the absence of specific protocols to extract histones from human articular cartilage has complicated this task. The lack of effective methods can be explained by the structural complexity and low cellularity of this tissue, which are responsible for the biomechanical properties that allow the movement of the joint but also complicate histone isolation. Here, we provide a histone extraction procedure specifically adapted for cryopreserved human articular cartilage that can be useful to understand epigenetic regulation in OA and accelerate the search for novel strategies. MDPI 2022-03-20 /pmc/articles/PMC8955682/ /pubmed/35328777 http://dx.doi.org/10.3390/ijms23063355 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Núñez-Carro, Carmen Blanco-Blanco, Margarita Montoya, Tatiana Villagrán-Andrade, Karla M. Hermida-Gómez, Tamara Blanco, Francisco J. de Andrés, María C. Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
title | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
title_full | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
title_fullStr | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
title_full_unstemmed | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
title_short | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
title_sort | histone extraction from human articular cartilage for the study of epigenetic regulation in osteoarthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955682/ https://www.ncbi.nlm.nih.gov/pubmed/35328777 http://dx.doi.org/10.3390/ijms23063355 |
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