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Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis

Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place betwe...

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Autores principales: Núñez-Carro, Carmen, Blanco-Blanco, Margarita, Montoya, Tatiana, Villagrán-Andrade, Karla M., Hermida-Gómez, Tamara, Blanco, Francisco J., de Andrés, María C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955682/
https://www.ncbi.nlm.nih.gov/pubmed/35328777
http://dx.doi.org/10.3390/ijms23063355
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author Núñez-Carro, Carmen
Blanco-Blanco, Margarita
Montoya, Tatiana
Villagrán-Andrade, Karla M.
Hermida-Gómez, Tamara
Blanco, Francisco J.
de Andrés, María C.
author_facet Núñez-Carro, Carmen
Blanco-Blanco, Margarita
Montoya, Tatiana
Villagrán-Andrade, Karla M.
Hermida-Gómez, Tamara
Blanco, Francisco J.
de Andrés, María C.
author_sort Núñez-Carro, Carmen
collection PubMed
description Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. OA chondrocytes enhance the production of interleukin 1β (IL-1β) and interleukin 8 (IL-8), which are epigenetically regulated. These cytokines upregulate the synthesis of matrix metalloproteinases (MMPs) and aggrecanases, which promote the extracellular matrix (ECM) destruction. This motivates the study of histone PTMs to investigate the epigenetic regulation of proinflammatory molecules, but the absence of specific protocols to extract histones from human articular cartilage has complicated this task. The lack of effective methods can be explained by the structural complexity and low cellularity of this tissue, which are responsible for the biomechanical properties that allow the movement of the joint but also complicate histone isolation. Here, we provide a histone extraction procedure specifically adapted for cryopreserved human articular cartilage that can be useful to understand epigenetic regulation in OA and accelerate the search for novel strategies.
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spelling pubmed-89556822022-03-26 Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis Núñez-Carro, Carmen Blanco-Blanco, Margarita Montoya, Tatiana Villagrán-Andrade, Karla M. Hermida-Gómez, Tamara Blanco, Francisco J. de Andrés, María C. Int J Mol Sci Article Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. OA chondrocytes enhance the production of interleukin 1β (IL-1β) and interleukin 8 (IL-8), which are epigenetically regulated. These cytokines upregulate the synthesis of matrix metalloproteinases (MMPs) and aggrecanases, which promote the extracellular matrix (ECM) destruction. This motivates the study of histone PTMs to investigate the epigenetic regulation of proinflammatory molecules, but the absence of specific protocols to extract histones from human articular cartilage has complicated this task. The lack of effective methods can be explained by the structural complexity and low cellularity of this tissue, which are responsible for the biomechanical properties that allow the movement of the joint but also complicate histone isolation. Here, we provide a histone extraction procedure specifically adapted for cryopreserved human articular cartilage that can be useful to understand epigenetic regulation in OA and accelerate the search for novel strategies. MDPI 2022-03-20 /pmc/articles/PMC8955682/ /pubmed/35328777 http://dx.doi.org/10.3390/ijms23063355 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Núñez-Carro, Carmen
Blanco-Blanco, Margarita
Montoya, Tatiana
Villagrán-Andrade, Karla M.
Hermida-Gómez, Tamara
Blanco, Francisco J.
de Andrés, María C.
Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
title Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
title_full Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
title_fullStr Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
title_full_unstemmed Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
title_short Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
title_sort histone extraction from human articular cartilage for the study of epigenetic regulation in osteoarthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955682/
https://www.ncbi.nlm.nih.gov/pubmed/35328777
http://dx.doi.org/10.3390/ijms23063355
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