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In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging

The aim of this study was to investigate the biocompatibility of contrast agents, such as gadolinium 1, 4, 7, 10 tetraazacyclo-dodecane tetraacetic acid (GdDOTA) and gadolinium dioctyl terephthalate (GdDOTP), encapsulated in a polymeric matrix containing chitosan and hyaluronic acid using RAW264.7 m...

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Autores principales: Gheran, Cecilia Virginia, Voicu, Sorina Nicoleta, Galateanu, Bianca, Callewaert, Maité, Moreau, Juliette, Cadiou, Cyril, Chuburu, Françoise, Dinischiotu, Anca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955704/
https://www.ncbi.nlm.nih.gov/pubmed/35328678
http://dx.doi.org/10.3390/ijms23063258
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author Gheran, Cecilia Virginia
Voicu, Sorina Nicoleta
Galateanu, Bianca
Callewaert, Maité
Moreau, Juliette
Cadiou, Cyril
Chuburu, Françoise
Dinischiotu, Anca
author_facet Gheran, Cecilia Virginia
Voicu, Sorina Nicoleta
Galateanu, Bianca
Callewaert, Maité
Moreau, Juliette
Cadiou, Cyril
Chuburu, Françoise
Dinischiotu, Anca
author_sort Gheran, Cecilia Virginia
collection PubMed
description The aim of this study was to investigate the biocompatibility of contrast agents, such as gadolinium 1, 4, 7, 10 tetraazacyclo-dodecane tetraacetic acid (GdDOTA) and gadolinium dioctyl terephthalate (GdDOTP), encapsulated in a polymeric matrix containing chitosan and hyaluronic acid using RAW264.7 murine macrophages and human blood samples. The cell viability and cytotoxicity were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays, while cell cycle analysis was determined in RAW264.7 cells using flow cytometry. The mitochondrial membrane potential (MMP), hemolytic index, complement activation, and thrombogenic potential of gadolinium (Gd) containing nanohydrogels were measured by fluorometric and spectrophotometric methods. Taken together, our results demonstrate the good bio- and hemocompatibility of chitosan-based nanohydrogels with the RAW264.7 cell line and human blood cells, suggesting that these could be used as injectable formulations for the magnetic resonance imaging diagnostic of lymph nodes.
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spelling pubmed-89557042022-03-26 In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging Gheran, Cecilia Virginia Voicu, Sorina Nicoleta Galateanu, Bianca Callewaert, Maité Moreau, Juliette Cadiou, Cyril Chuburu, Françoise Dinischiotu, Anca Int J Mol Sci Article The aim of this study was to investigate the biocompatibility of contrast agents, such as gadolinium 1, 4, 7, 10 tetraazacyclo-dodecane tetraacetic acid (GdDOTA) and gadolinium dioctyl terephthalate (GdDOTP), encapsulated in a polymeric matrix containing chitosan and hyaluronic acid using RAW264.7 murine macrophages and human blood samples. The cell viability and cytotoxicity were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays, while cell cycle analysis was determined in RAW264.7 cells using flow cytometry. The mitochondrial membrane potential (MMP), hemolytic index, complement activation, and thrombogenic potential of gadolinium (Gd) containing nanohydrogels were measured by fluorometric and spectrophotometric methods. Taken together, our results demonstrate the good bio- and hemocompatibility of chitosan-based nanohydrogels with the RAW264.7 cell line and human blood cells, suggesting that these could be used as injectable formulations for the magnetic resonance imaging diagnostic of lymph nodes. MDPI 2022-03-17 /pmc/articles/PMC8955704/ /pubmed/35328678 http://dx.doi.org/10.3390/ijms23063258 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gheran, Cecilia Virginia
Voicu, Sorina Nicoleta
Galateanu, Bianca
Callewaert, Maité
Moreau, Juliette
Cadiou, Cyril
Chuburu, Françoise
Dinischiotu, Anca
In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging
title In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging
title_full In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging
title_fullStr In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging
title_full_unstemmed In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging
title_short In Vitro Studies Regarding the Safety of Chitosan and Hyaluronic Acid-Based Nanohydrogels Containing Contrast Agents for Magnetic Resonance Imaging
title_sort in vitro studies regarding the safety of chitosan and hyaluronic acid-based nanohydrogels containing contrast agents for magnetic resonance imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955704/
https://www.ncbi.nlm.nih.gov/pubmed/35328678
http://dx.doi.org/10.3390/ijms23063258
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