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Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955835/ https://www.ncbi.nlm.nih.gov/pubmed/35336081 http://dx.doi.org/10.3390/microorganisms10030505 |
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author | Attia, Hanene Sghaier, Manel Rabia Bali, Aymen Guerfali, Fatma Zahra Chlif, Sadok Atri, Chiraz Belhaj-Hamida, Nabil Zaatour, Amor Gharbi, Adel Ben-Salah, Afif Dellagi, Koussay Laouini, Dhafer |
author_facet | Attia, Hanene Sghaier, Manel Rabia Bali, Aymen Guerfali, Fatma Zahra Chlif, Sadok Atri, Chiraz Belhaj-Hamida, Nabil Zaatour, Amor Gharbi, Adel Ben-Salah, Afif Dellagi, Koussay Laouini, Dhafer |
author_sort | Attia, Hanene |
collection | PubMed |
description | The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of bites, genetic variation between L. major isolates might impact on the clinical output of the disease. We investigated herein the intra-specific variability of L. major field isolates independently of host or vector factors and then tried to correlate parasite variability to ZCL severity in corresponding patients. Several assays were applied, i.e., in vivo pathogenicity of promastigotes in a BALB/c mice model, resistance/sensibility to complement lysis, in vitro growth kinetics, and expression of different lectins on the promastigote surface. Combining all these parameters allowed us to conclude that the resistance to complement lysis and PNA/Jacalin lectins binding to parasite surfaces are important markers of parasite virulence. These factors correlate significantly with clinic polymorphism of ZCL and modestly with genetic micro-heterogeneity, a characteristic we previously revealed with a MLMT profile. |
format | Online Article Text |
id | pubmed-8955835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89558352022-03-26 Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism Attia, Hanene Sghaier, Manel Rabia Bali, Aymen Guerfali, Fatma Zahra Chlif, Sadok Atri, Chiraz Belhaj-Hamida, Nabil Zaatour, Amor Gharbi, Adel Ben-Salah, Afif Dellagi, Koussay Laouini, Dhafer Microorganisms Article The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of bites, genetic variation between L. major isolates might impact on the clinical output of the disease. We investigated herein the intra-specific variability of L. major field isolates independently of host or vector factors and then tried to correlate parasite variability to ZCL severity in corresponding patients. Several assays were applied, i.e., in vivo pathogenicity of promastigotes in a BALB/c mice model, resistance/sensibility to complement lysis, in vitro growth kinetics, and expression of different lectins on the promastigote surface. Combining all these parameters allowed us to conclude that the resistance to complement lysis and PNA/Jacalin lectins binding to parasite surfaces are important markers of parasite virulence. These factors correlate significantly with clinic polymorphism of ZCL and modestly with genetic micro-heterogeneity, a characteristic we previously revealed with a MLMT profile. MDPI 2022-02-25 /pmc/articles/PMC8955835/ /pubmed/35336081 http://dx.doi.org/10.3390/microorganisms10030505 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Attia, Hanene Sghaier, Manel Rabia Bali, Aymen Guerfali, Fatma Zahra Chlif, Sadok Atri, Chiraz Belhaj-Hamida, Nabil Zaatour, Amor Gharbi, Adel Ben-Salah, Afif Dellagi, Koussay Laouini, Dhafer Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism |
title | Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism |
title_full | Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism |
title_fullStr | Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism |
title_full_unstemmed | Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism |
title_short | Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism |
title_sort | intra-specific diversity of leishmania major isolates: a key determinant of tunisian zoonotic cutaneous leishmaniasis clinical polymorphism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955835/ https://www.ncbi.nlm.nih.gov/pubmed/35336081 http://dx.doi.org/10.3390/microorganisms10030505 |
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