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Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism

The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of...

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Autores principales: Attia, Hanene, Sghaier, Manel Rabia, Bali, Aymen, Guerfali, Fatma Zahra, Chlif, Sadok, Atri, Chiraz, Belhaj-Hamida, Nabil, Zaatour, Amor, Gharbi, Adel, Ben-Salah, Afif, Dellagi, Koussay, Laouini, Dhafer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955835/
https://www.ncbi.nlm.nih.gov/pubmed/35336081
http://dx.doi.org/10.3390/microorganisms10030505
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author Attia, Hanene
Sghaier, Manel Rabia
Bali, Aymen
Guerfali, Fatma Zahra
Chlif, Sadok
Atri, Chiraz
Belhaj-Hamida, Nabil
Zaatour, Amor
Gharbi, Adel
Ben-Salah, Afif
Dellagi, Koussay
Laouini, Dhafer
author_facet Attia, Hanene
Sghaier, Manel Rabia
Bali, Aymen
Guerfali, Fatma Zahra
Chlif, Sadok
Atri, Chiraz
Belhaj-Hamida, Nabil
Zaatour, Amor
Gharbi, Adel
Ben-Salah, Afif
Dellagi, Koussay
Laouini, Dhafer
author_sort Attia, Hanene
collection PubMed
description The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of bites, genetic variation between L. major isolates might impact on the clinical output of the disease. We investigated herein the intra-specific variability of L. major field isolates independently of host or vector factors and then tried to correlate parasite variability to ZCL severity in corresponding patients. Several assays were applied, i.e., in vivo pathogenicity of promastigotes in a BALB/c mice model, resistance/sensibility to complement lysis, in vitro growth kinetics, and expression of different lectins on the promastigote surface. Combining all these parameters allowed us to conclude that the resistance to complement lysis and PNA/Jacalin lectins binding to parasite surfaces are important markers of parasite virulence. These factors correlate significantly with clinic polymorphism of ZCL and modestly with genetic micro-heterogeneity, a characteristic we previously revealed with a MLMT profile.
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spelling pubmed-89558352022-03-26 Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism Attia, Hanene Sghaier, Manel Rabia Bali, Aymen Guerfali, Fatma Zahra Chlif, Sadok Atri, Chiraz Belhaj-Hamida, Nabil Zaatour, Amor Gharbi, Adel Ben-Salah, Afif Dellagi, Koussay Laouini, Dhafer Microorganisms Article The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of bites, genetic variation between L. major isolates might impact on the clinical output of the disease. We investigated herein the intra-specific variability of L. major field isolates independently of host or vector factors and then tried to correlate parasite variability to ZCL severity in corresponding patients. Several assays were applied, i.e., in vivo pathogenicity of promastigotes in a BALB/c mice model, resistance/sensibility to complement lysis, in vitro growth kinetics, and expression of different lectins on the promastigote surface. Combining all these parameters allowed us to conclude that the resistance to complement lysis and PNA/Jacalin lectins binding to parasite surfaces are important markers of parasite virulence. These factors correlate significantly with clinic polymorphism of ZCL and modestly with genetic micro-heterogeneity, a characteristic we previously revealed with a MLMT profile. MDPI 2022-02-25 /pmc/articles/PMC8955835/ /pubmed/35336081 http://dx.doi.org/10.3390/microorganisms10030505 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Attia, Hanene
Sghaier, Manel Rabia
Bali, Aymen
Guerfali, Fatma Zahra
Chlif, Sadok
Atri, Chiraz
Belhaj-Hamida, Nabil
Zaatour, Amor
Gharbi, Adel
Ben-Salah, Afif
Dellagi, Koussay
Laouini, Dhafer
Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
title Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
title_full Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
title_fullStr Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
title_full_unstemmed Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
title_short Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism
title_sort intra-specific diversity of leishmania major isolates: a key determinant of tunisian zoonotic cutaneous leishmaniasis clinical polymorphism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955835/
https://www.ncbi.nlm.nih.gov/pubmed/35336081
http://dx.doi.org/10.3390/microorganisms10030505
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