Cargando…
Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment
Lung cancer is the most common cancer in the world and several miRNAs are associated with it. MiRNA sponges are presented as tools to inhibit miRNAs. We designed a system to capture miRNAs based on circular RNAs (circRNA). To demonstrate its usefulness, we chose miR-21, which is upregulated and impl...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955967/ https://www.ncbi.nlm.nih.gov/pubmed/35328383 http://dx.doi.org/10.3390/ijms23062963 |
_version_ | 1784676465918345216 |
---|---|
author | Rama, Ana R. Quiñonero, Francisco Mesas, Cristina Melguizo, Consolación Prados, Jose |
author_facet | Rama, Ana R. Quiñonero, Francisco Mesas, Cristina Melguizo, Consolación Prados, Jose |
author_sort | Rama, Ana R. |
collection | PubMed |
description | Lung cancer is the most common cancer in the world and several miRNAs are associated with it. MiRNA sponges are presented as tools to inhibit miRNAs. We designed a system to capture miRNAs based on circular RNAs (circRNA). To demonstrate its usefulness, we chose miR-21, which is upregulated and implicated in lung cancer. We constructed a miR-21 sponge and inserted it into a vector that facilitates circular RNA production (Circ-21) to study its effect on growth, colony formation, and migration in lung cancer cell lines and multicellular tumor spheroids (MTS). Circ-21 induced a significant and time-dependent decrease in the growth of A549 and LL2 cells, but not in L132 cells. Furthermore, A549 and LL2 cells transfected with Circ-21 showed a lower number of colonies and migration than L132. Similar findings were seen in A549 and LL2 Circ-21 MTS, which showed a significant decrease in volume growth, but not in L132 Circ-21 MTS. Based on this, the miR-21 circular sponge may suppress the processes of tumorigenesis and progression. Therefore, our system based on circular sponges seems to be effective, as a tool for the capture of other miRNAs. |
format | Online Article Text |
id | pubmed-8955967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89559672022-03-26 Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment Rama, Ana R. Quiñonero, Francisco Mesas, Cristina Melguizo, Consolación Prados, Jose Int J Mol Sci Article Lung cancer is the most common cancer in the world and several miRNAs are associated with it. MiRNA sponges are presented as tools to inhibit miRNAs. We designed a system to capture miRNAs based on circular RNAs (circRNA). To demonstrate its usefulness, we chose miR-21, which is upregulated and implicated in lung cancer. We constructed a miR-21 sponge and inserted it into a vector that facilitates circular RNA production (Circ-21) to study its effect on growth, colony formation, and migration in lung cancer cell lines and multicellular tumor spheroids (MTS). Circ-21 induced a significant and time-dependent decrease in the growth of A549 and LL2 cells, but not in L132 cells. Furthermore, A549 and LL2 cells transfected with Circ-21 showed a lower number of colonies and migration than L132. Similar findings were seen in A549 and LL2 Circ-21 MTS, which showed a significant decrease in volume growth, but not in L132 Circ-21 MTS. Based on this, the miR-21 circular sponge may suppress the processes of tumorigenesis and progression. Therefore, our system based on circular sponges seems to be effective, as a tool for the capture of other miRNAs. MDPI 2022-03-09 /pmc/articles/PMC8955967/ /pubmed/35328383 http://dx.doi.org/10.3390/ijms23062963 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rama, Ana R. Quiñonero, Francisco Mesas, Cristina Melguizo, Consolación Prados, Jose Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
title | Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
title_full | Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
title_fullStr | Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
title_full_unstemmed | Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
title_short | Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment |
title_sort | synthetic circular mir-21 sponge as tool for lung cancer treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955967/ https://www.ncbi.nlm.nih.gov/pubmed/35328383 http://dx.doi.org/10.3390/ijms23062963 |
work_keys_str_mv | AT ramaanar syntheticcircularmir21spongeastoolforlungcancertreatment AT quinonerofrancisco syntheticcircularmir21spongeastoolforlungcancertreatment AT mesascristina syntheticcircularmir21spongeastoolforlungcancertreatment AT melguizoconsolacion syntheticcircularmir21spongeastoolforlungcancertreatment AT pradosjose syntheticcircularmir21spongeastoolforlungcancertreatment |