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REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish

REV-ERBα (nr1d1, nuclear receptor subfamily 1 group D member 1) is a transcriptional repressor that in mammals regulates nutrient metabolism, and has effects on energy homeostasis, although its role in teleosts is poorly understood. To determine REV-ERBα’s involvement in fish energy balance and meta...

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Autores principales: Saiz, Nuria, Herrera-Castillo, Lisbeth, Isorna, Esther, Delgado, María Jesús, Conde-Sieira, Marta, Soengas, José Luis, de Pedro, Nuria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955992/
https://www.ncbi.nlm.nih.gov/pubmed/35328345
http://dx.doi.org/10.3390/ijms23062921
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author Saiz, Nuria
Herrera-Castillo, Lisbeth
Isorna, Esther
Delgado, María Jesús
Conde-Sieira, Marta
Soengas, José Luis
de Pedro, Nuria
author_facet Saiz, Nuria
Herrera-Castillo, Lisbeth
Isorna, Esther
Delgado, María Jesús
Conde-Sieira, Marta
Soengas, José Luis
de Pedro, Nuria
author_sort Saiz, Nuria
collection PubMed
description REV-ERBα (nr1d1, nuclear receptor subfamily 1 group D member 1) is a transcriptional repressor that in mammals regulates nutrient metabolism, and has effects on energy homeostasis, although its role in teleosts is poorly understood. To determine REV-ERBα’s involvement in fish energy balance and metabolism, we studied the effects of acute and 7-day administration of its agonist SR9009 on food intake, weight and length gain, locomotor activity, feeding regulators, plasma and hepatic metabolites, and liver enzymatic activity. SR9009 inhibited feeding, lowering body weight and length gain. In addition, the abundance of ghrelin mRNA decreased in the intestine, and abundance of leptin-aI mRNA increased in the liver. Hypocretin, neuropeptide y (npy), and proopiomelanocortin (pomc) mRNA abundance was not modified after acute or subchronic SR9009 administration, while hypothalamic cocaine- and amphetamine-regulated transcript (cartpt-I) was induced in the subchronic treatment, being a possible mediator of the anorectic effects. Moreover, SR9009 decreased plasma glucose, coinciding with increased glycolysis and a decreased gluconeogenesis in the liver. Decreased triglyceride levels and activity of lipogenic enzymes suggest a lipogenesis reduction by SR9009. Energy expenditure by locomotor activity was not significantly affected by SR9009. Overall, this study shows for the first time in fish the effects of REV-ERBα activation via SR9009, promoting a negative energy balance by reducing energetic inputs and regulating lipid and glucose metabolism.
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spelling pubmed-89559922022-03-26 REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish Saiz, Nuria Herrera-Castillo, Lisbeth Isorna, Esther Delgado, María Jesús Conde-Sieira, Marta Soengas, José Luis de Pedro, Nuria Int J Mol Sci Article REV-ERBα (nr1d1, nuclear receptor subfamily 1 group D member 1) is a transcriptional repressor that in mammals regulates nutrient metabolism, and has effects on energy homeostasis, although its role in teleosts is poorly understood. To determine REV-ERBα’s involvement in fish energy balance and metabolism, we studied the effects of acute and 7-day administration of its agonist SR9009 on food intake, weight and length gain, locomotor activity, feeding regulators, plasma and hepatic metabolites, and liver enzymatic activity. SR9009 inhibited feeding, lowering body weight and length gain. In addition, the abundance of ghrelin mRNA decreased in the intestine, and abundance of leptin-aI mRNA increased in the liver. Hypocretin, neuropeptide y (npy), and proopiomelanocortin (pomc) mRNA abundance was not modified after acute or subchronic SR9009 administration, while hypothalamic cocaine- and amphetamine-regulated transcript (cartpt-I) was induced in the subchronic treatment, being a possible mediator of the anorectic effects. Moreover, SR9009 decreased plasma glucose, coinciding with increased glycolysis and a decreased gluconeogenesis in the liver. Decreased triglyceride levels and activity of lipogenic enzymes suggest a lipogenesis reduction by SR9009. Energy expenditure by locomotor activity was not significantly affected by SR9009. Overall, this study shows for the first time in fish the effects of REV-ERBα activation via SR9009, promoting a negative energy balance by reducing energetic inputs and regulating lipid and glucose metabolism. MDPI 2022-03-08 /pmc/articles/PMC8955992/ /pubmed/35328345 http://dx.doi.org/10.3390/ijms23062921 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saiz, Nuria
Herrera-Castillo, Lisbeth
Isorna, Esther
Delgado, María Jesús
Conde-Sieira, Marta
Soengas, José Luis
de Pedro, Nuria
REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
title REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
title_full REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
title_fullStr REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
title_full_unstemmed REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
title_short REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
title_sort rev-erbα agonist sr9009 promotes a negative energy balance in goldfish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955992/
https://www.ncbi.nlm.nih.gov/pubmed/35328345
http://dx.doi.org/10.3390/ijms23062921
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