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Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
Background: Contamination of the hospital environment with multi-resistant (MDR) Staphylococcus increases the risk of infection. The aim of this study is to identify the MDR species of Staphylococcus on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955995/ https://www.ncbi.nlm.nih.gov/pubmed/35336141 http://dx.doi.org/10.3390/microorganisms10030567 |
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author | Menezes, Ralciane de Paula Marques, Lara de Andrade Silva, Felipe Flávio Silva, Nagela Bernadelli Sousa Alves, Priscila Guerino Vilela Bessa, Meliza Arantes de Souza de Araújo, Lúcio Borges Penatti, Mário Paulo Amante Pedroso, Reginaldo dos Santos de Brito Röder, Denise Von Dolinger |
author_facet | Menezes, Ralciane de Paula Marques, Lara de Andrade Silva, Felipe Flávio Silva, Nagela Bernadelli Sousa Alves, Priscila Guerino Vilela Bessa, Meliza Arantes de Souza de Araújo, Lúcio Borges Penatti, Mário Paulo Amante Pedroso, Reginaldo dos Santos de Brito Röder, Denise Von Dolinger |
author_sort | Menezes, Ralciane de Paula |
collection | PubMed |
description | Background: Contamination of the hospital environment with multi-resistant (MDR) Staphylococcus increases the risk of infection. The aim of this study is to identify the MDR species of Staphylococcus on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate with the occurrence of infections in a Neonatal Intensive Care Unit. Methods: Samples of inanimate surfaces and air were taken using a premoistened swab (0.9% sodium chloride) and spontaneous air sedimentation, respectively. The clinical isolates were recovered from infected neonates. The isolates (environmental and clinical) were identified by matrix-assisted laser desorption ionization-time of flight and the resistance profile was calculated using the disk diffusion agar technique. Results: In total, 181 isolates were obtained, 93 from (surfaces), 18 from the air, and 70 clinical samples. S. epidermidis was the most frequent species (66.8%), and the failure rate in air cleaning was 100%. More than 60% of the isolates were MDR, and the majority of clinical isolates (60.4%) had a resistance profile identical to that of the environmental isolates. Conclusion: Staphylococcus spp. were found in most of the analyzed samples, with a high frequency of MDR isolates, demonstrating the importance of the hospital environment as a reservoir, and the need for infection control measures, and rational use of antimicrobials. |
format | Online Article Text |
id | pubmed-8955995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89559952022-03-26 Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment Menezes, Ralciane de Paula Marques, Lara de Andrade Silva, Felipe Flávio Silva, Nagela Bernadelli Sousa Alves, Priscila Guerino Vilela Bessa, Meliza Arantes de Souza de Araújo, Lúcio Borges Penatti, Mário Paulo Amante Pedroso, Reginaldo dos Santos de Brito Röder, Denise Von Dolinger Microorganisms Article Background: Contamination of the hospital environment with multi-resistant (MDR) Staphylococcus increases the risk of infection. The aim of this study is to identify the MDR species of Staphylococcus on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate with the occurrence of infections in a Neonatal Intensive Care Unit. Methods: Samples of inanimate surfaces and air were taken using a premoistened swab (0.9% sodium chloride) and spontaneous air sedimentation, respectively. The clinical isolates were recovered from infected neonates. The isolates (environmental and clinical) were identified by matrix-assisted laser desorption ionization-time of flight and the resistance profile was calculated using the disk diffusion agar technique. Results: In total, 181 isolates were obtained, 93 from (surfaces), 18 from the air, and 70 clinical samples. S. epidermidis was the most frequent species (66.8%), and the failure rate in air cleaning was 100%. More than 60% of the isolates were MDR, and the majority of clinical isolates (60.4%) had a resistance profile identical to that of the environmental isolates. Conclusion: Staphylococcus spp. were found in most of the analyzed samples, with a high frequency of MDR isolates, demonstrating the importance of the hospital environment as a reservoir, and the need for infection control measures, and rational use of antimicrobials. MDPI 2022-03-05 /pmc/articles/PMC8955995/ /pubmed/35336141 http://dx.doi.org/10.3390/microorganisms10030567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Menezes, Ralciane de Paula Marques, Lara de Andrade Silva, Felipe Flávio Silva, Nagela Bernadelli Sousa Alves, Priscila Guerino Vilela Bessa, Meliza Arantes de Souza de Araújo, Lúcio Borges Penatti, Mário Paulo Amante Pedroso, Reginaldo dos Santos de Brito Röder, Denise Von Dolinger Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment |
title | Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment |
title_full | Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment |
title_fullStr | Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment |
title_full_unstemmed | Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment |
title_short | Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment |
title_sort | inanimate surfaces and air contamination with multidrug resistant species of staphylococcus in the neonatal intensive care unit environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955995/ https://www.ncbi.nlm.nih.gov/pubmed/35336141 http://dx.doi.org/10.3390/microorganisms10030567 |
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