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Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment

Background: Contamination of the hospital environment with multi-resistant (MDR) Staphylococcus increases the risk of infection. The aim of this study is to identify the MDR species of Staphylococcus on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate...

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Autores principales: Menezes, Ralciane de Paula, Marques, Lara de Andrade, Silva, Felipe Flávio, Silva, Nagela Bernadelli Sousa, Alves, Priscila Guerino Vilela, Bessa, Meliza Arantes de Souza, de Araújo, Lúcio Borges, Penatti, Mário Paulo Amante, Pedroso, Reginaldo dos Santos, de Brito Röder, Denise Von Dolinger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955995/
https://www.ncbi.nlm.nih.gov/pubmed/35336141
http://dx.doi.org/10.3390/microorganisms10030567
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author Menezes, Ralciane de Paula
Marques, Lara de Andrade
Silva, Felipe Flávio
Silva, Nagela Bernadelli Sousa
Alves, Priscila Guerino Vilela
Bessa, Meliza Arantes de Souza
de Araújo, Lúcio Borges
Penatti, Mário Paulo Amante
Pedroso, Reginaldo dos Santos
de Brito Röder, Denise Von Dolinger
author_facet Menezes, Ralciane de Paula
Marques, Lara de Andrade
Silva, Felipe Flávio
Silva, Nagela Bernadelli Sousa
Alves, Priscila Guerino Vilela
Bessa, Meliza Arantes de Souza
de Araújo, Lúcio Borges
Penatti, Mário Paulo Amante
Pedroso, Reginaldo dos Santos
de Brito Röder, Denise Von Dolinger
author_sort Menezes, Ralciane de Paula
collection PubMed
description Background: Contamination of the hospital environment with multi-resistant (MDR) Staphylococcus increases the risk of infection. The aim of this study is to identify the MDR species of Staphylococcus on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate with the occurrence of infections in a Neonatal Intensive Care Unit. Methods: Samples of inanimate surfaces and air were taken using a premoistened swab (0.9% sodium chloride) and spontaneous air sedimentation, respectively. The clinical isolates were recovered from infected neonates. The isolates (environmental and clinical) were identified by matrix-assisted laser desorption ionization-time of flight and the resistance profile was calculated using the disk diffusion agar technique. Results: In total, 181 isolates were obtained, 93 from (surfaces), 18 from the air, and 70 clinical samples. S. epidermidis was the most frequent species (66.8%), and the failure rate in air cleaning was 100%. More than 60% of the isolates were MDR, and the majority of clinical isolates (60.4%) had a resistance profile identical to that of the environmental isolates. Conclusion: Staphylococcus spp. were found in most of the analyzed samples, with a high frequency of MDR isolates, demonstrating the importance of the hospital environment as a reservoir, and the need for infection control measures, and rational use of antimicrobials.
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spelling pubmed-89559952022-03-26 Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment Menezes, Ralciane de Paula Marques, Lara de Andrade Silva, Felipe Flávio Silva, Nagela Bernadelli Sousa Alves, Priscila Guerino Vilela Bessa, Meliza Arantes de Souza de Araújo, Lúcio Borges Penatti, Mário Paulo Amante Pedroso, Reginaldo dos Santos de Brito Röder, Denise Von Dolinger Microorganisms Article Background: Contamination of the hospital environment with multi-resistant (MDR) Staphylococcus increases the risk of infection. The aim of this study is to identify the MDR species of Staphylococcus on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate with the occurrence of infections in a Neonatal Intensive Care Unit. Methods: Samples of inanimate surfaces and air were taken using a premoistened swab (0.9% sodium chloride) and spontaneous air sedimentation, respectively. The clinical isolates were recovered from infected neonates. The isolates (environmental and clinical) were identified by matrix-assisted laser desorption ionization-time of flight and the resistance profile was calculated using the disk diffusion agar technique. Results: In total, 181 isolates were obtained, 93 from (surfaces), 18 from the air, and 70 clinical samples. S. epidermidis was the most frequent species (66.8%), and the failure rate in air cleaning was 100%. More than 60% of the isolates were MDR, and the majority of clinical isolates (60.4%) had a resistance profile identical to that of the environmental isolates. Conclusion: Staphylococcus spp. were found in most of the analyzed samples, with a high frequency of MDR isolates, demonstrating the importance of the hospital environment as a reservoir, and the need for infection control measures, and rational use of antimicrobials. MDPI 2022-03-05 /pmc/articles/PMC8955995/ /pubmed/35336141 http://dx.doi.org/10.3390/microorganisms10030567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Menezes, Ralciane de Paula
Marques, Lara de Andrade
Silva, Felipe Flávio
Silva, Nagela Bernadelli Sousa
Alves, Priscila Guerino Vilela
Bessa, Meliza Arantes de Souza
de Araújo, Lúcio Borges
Penatti, Mário Paulo Amante
Pedroso, Reginaldo dos Santos
de Brito Röder, Denise Von Dolinger
Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
title Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
title_full Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
title_fullStr Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
title_full_unstemmed Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
title_short Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of Staphylococcus in the Neonatal Intensive Care Unit Environment
title_sort inanimate surfaces and air contamination with multidrug resistant species of staphylococcus in the neonatal intensive care unit environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955995/
https://www.ncbi.nlm.nih.gov/pubmed/35336141
http://dx.doi.org/10.3390/microorganisms10030567
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