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Universal buffers for use in biochemistry and biophysical experiments
The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins. Buffer molecules can induce changes in conformational equilibria, dynamic behavior, and catalytic proper...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956001/ https://www.ncbi.nlm.nih.gov/pubmed/35340547 http://dx.doi.org/10.3934/biophy.2015.3.336 |
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author | Brooke, Dewey Movahed, Navid Bothner, Brian |
author_facet | Brooke, Dewey Movahed, Navid Bothner, Brian |
author_sort | Brooke, Dewey |
collection | PubMed |
description | The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins. Buffer molecules can induce changes in conformational equilibria, dynamic behavior, and catalytic properties merely by their presence in solution. This effect is of concern because many of the standard experiments used to investigate protein structure and function involve changing solution conditions such as pH and/or temperature. In experiments in which pH is varied, it is common practice to switch buffering agents so that the pH is within the working range of the weak acid and conjugate base. If multiple buffers are used, it is not always possible to decouple buffer induced change from pH or temperature induced change. We have developed a series of mixed biological buffers for protein analysis that can be used across a broad pH range, are compatible with biologically relevant metal ions, and avoid complications that may arise from changing the small molecule composition of buffers when pH is used as an experimental variable. |
format | Online Article Text |
id | pubmed-8956001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-89560012022-03-25 Universal buffers for use in biochemistry and biophysical experiments Brooke, Dewey Movahed, Navid Bothner, Brian AIMS Biophys Article The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins. Buffer molecules can induce changes in conformational equilibria, dynamic behavior, and catalytic properties merely by their presence in solution. This effect is of concern because many of the standard experiments used to investigate protein structure and function involve changing solution conditions such as pH and/or temperature. In experiments in which pH is varied, it is common practice to switch buffering agents so that the pH is within the working range of the weak acid and conjugate base. If multiple buffers are used, it is not always possible to decouple buffer induced change from pH or temperature induced change. We have developed a series of mixed biological buffers for protein analysis that can be used across a broad pH range, are compatible with biologically relevant metal ions, and avoid complications that may arise from changing the small molecule composition of buffers when pH is used as an experimental variable. 2015 2015-08-14 /pmc/articles/PMC8956001/ /pubmed/35340547 http://dx.doi.org/10.3934/biophy.2015.3.336 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ) |
spellingShingle | Article Brooke, Dewey Movahed, Navid Bothner, Brian Universal buffers for use in biochemistry and biophysical experiments |
title | Universal buffers for use in biochemistry and biophysical experiments |
title_full | Universal buffers for use in biochemistry and biophysical experiments |
title_fullStr | Universal buffers for use in biochemistry and biophysical experiments |
title_full_unstemmed | Universal buffers for use in biochemistry and biophysical experiments |
title_short | Universal buffers for use in biochemistry and biophysical experiments |
title_sort | universal buffers for use in biochemistry and biophysical experiments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956001/ https://www.ncbi.nlm.nih.gov/pubmed/35340547 http://dx.doi.org/10.3934/biophy.2015.3.336 |
work_keys_str_mv | AT brookedewey universalbuffersforuseinbiochemistryandbiophysicalexperiments AT movahednavid universalbuffersforuseinbiochemistryandbiophysicalexperiments AT bothnerbrian universalbuffersforuseinbiochemistryandbiophysicalexperiments |