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Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis

The role played by hereditary factors in the development of diabetes mellitus type 2 (DM2) has not yet been fully established. Therefore, the purpose of our study was to investigate the prevalence of adiponectin and polymorphism in its gene receptors in connection with the primary symptoms of DM2 pa...

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Autores principales: Shramko, Iuliana, Ageeva, Elizaveta, Krutikov, Eugene, Maliy, Konstantin, Repinskaya, Irina, Fomochkina, Iryna, Kubishkin, Anatolii, Gurtovaya, Anna, Tarimov, Cyrill, Shekhar, Suman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956057/
https://www.ncbi.nlm.nih.gov/pubmed/35366291
http://dx.doi.org/10.3390/pathophysiology29010008
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author Shramko, Iuliana
Ageeva, Elizaveta
Krutikov, Eugene
Maliy, Konstantin
Repinskaya, Irina
Fomochkina, Iryna
Kubishkin, Anatolii
Gurtovaya, Anna
Tarimov, Cyrill
Shekhar, Suman
author_facet Shramko, Iuliana
Ageeva, Elizaveta
Krutikov, Eugene
Maliy, Konstantin
Repinskaya, Irina
Fomochkina, Iryna
Kubishkin, Anatolii
Gurtovaya, Anna
Tarimov, Cyrill
Shekhar, Suman
author_sort Shramko, Iuliana
collection PubMed
description The role played by hereditary factors in the development of diabetes mellitus type 2 (DM2) has not yet been fully established. Therefore, the purpose of our study was to investigate the prevalence of adiponectin and polymorphism in its gene receptors in connection with the primary symptoms of DM2 pathogenesis. Genomic DNA was isolated from the whole blood of 94 patients with an established diagnosis of DM2 using the phenol–chloroform method. Gene polymorphisms were determined using real-time polymerase chain reaction (PCR). The most common polymorphic variants in patients with DM2 were the genotypes AA (rs11061971) and GG (rs16928751) on the ADIPOR2 gene. A strong correlation was found between the rs16928751 polymorphism on the ADIPOR2 gene and increased body mass index (BMI). TG (rs2275737) ADIPOR1 gene genotype carriers were found to have the highest levels of glycosylated hemoglobin (HbA1), whereas TT (rs2275738) caused stable hyperglycemia. In addition, the rs16928751 ADIPOR2 gene polymorphism showed an association with the development of key mechanisms of DM2 in the Russian population, although a number of genomic searches failed to show any association of this gene with DM2. Unique gene variants associated with the risk of developing DM2 in the Crimean population were established.
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spelling pubmed-89560572022-03-26 Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis Shramko, Iuliana Ageeva, Elizaveta Krutikov, Eugene Maliy, Konstantin Repinskaya, Irina Fomochkina, Iryna Kubishkin, Anatolii Gurtovaya, Anna Tarimov, Cyrill Shekhar, Suman Pathophysiology Article The role played by hereditary factors in the development of diabetes mellitus type 2 (DM2) has not yet been fully established. Therefore, the purpose of our study was to investigate the prevalence of adiponectin and polymorphism in its gene receptors in connection with the primary symptoms of DM2 pathogenesis. Genomic DNA was isolated from the whole blood of 94 patients with an established diagnosis of DM2 using the phenol–chloroform method. Gene polymorphisms were determined using real-time polymerase chain reaction (PCR). The most common polymorphic variants in patients with DM2 were the genotypes AA (rs11061971) and GG (rs16928751) on the ADIPOR2 gene. A strong correlation was found between the rs16928751 polymorphism on the ADIPOR2 gene and increased body mass index (BMI). TG (rs2275737) ADIPOR1 gene genotype carriers were found to have the highest levels of glycosylated hemoglobin (HbA1), whereas TT (rs2275738) caused stable hyperglycemia. In addition, the rs16928751 ADIPOR2 gene polymorphism showed an association with the development of key mechanisms of DM2 in the Russian population, although a number of genomic searches failed to show any association of this gene with DM2. Unique gene variants associated with the risk of developing DM2 in the Crimean population were established. MDPI 2022-02-28 /pmc/articles/PMC8956057/ /pubmed/35366291 http://dx.doi.org/10.3390/pathophysiology29010008 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shramko, Iuliana
Ageeva, Elizaveta
Krutikov, Eugene
Maliy, Konstantin
Repinskaya, Irina
Fomochkina, Iryna
Kubishkin, Anatolii
Gurtovaya, Anna
Tarimov, Cyrill
Shekhar, Suman
Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis
title Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis
title_full Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis
title_fullStr Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis
title_full_unstemmed Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis
title_short Polymorphism in Adiponectin and Adiponectin Receptor Genes in Diabetes Mellitus Pathogenesis
title_sort polymorphism in adiponectin and adiponectin receptor genes in diabetes mellitus pathogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956057/
https://www.ncbi.nlm.nih.gov/pubmed/35366291
http://dx.doi.org/10.3390/pathophysiology29010008
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