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Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote

Previously, we reported that the Polo-like kinase PLK-1 phosphorylates the single Caenorhabditis elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed that this event is required to form a pronuclear envelope scission event that removes membranes on the juxtaposed oocyte...

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Autores principales: Velez-Aguilera, Griselda, Ossareh-Nazari, Batool, Van Hove, Lucie, Joly, Nicolas, Pintard, Lionel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956289/
https://www.ncbi.nlm.nih.gov/pubmed/35259092
http://dx.doi.org/10.7554/eLife.75382
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author Velez-Aguilera, Griselda
Ossareh-Nazari, Batool
Van Hove, Lucie
Joly, Nicolas
Pintard, Lionel
author_facet Velez-Aguilera, Griselda
Ossareh-Nazari, Batool
Van Hove, Lucie
Joly, Nicolas
Pintard, Lionel
author_sort Velez-Aguilera, Griselda
collection PubMed
description Previously, we reported that the Polo-like kinase PLK-1 phosphorylates the single Caenorhabditis elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed that this event is required to form a pronuclear envelope scission event that removes membranes on the juxtaposed oocyte and sperm pronuclear envelopes in the zygote, allowing the parental chromosomes to merge in a single nucleus after segregation (Velez-Aguilera et al., 2020). Here, we show that cortical microtubule pulling forces contribute to pronuclear envelopes scission by promoting mitotic spindle elongation, and conversely, nuclear envelopes remodeling facilitates spindle elongation. We also demonstrate that weakening the pronuclear envelopes via PLK-1-mediated lamina depolymerization, is a prerequisite for the astral microtubule pulling forces to trigger pronuclear membranes scission. Finally, we provide evidence that PLK-1 mainly acts via lamina depolymerization in this process. These observations thus indicate that temporal coordination between lamina depolymerization and mitotic spindle elongation facilitates pronuclear envelopes scission and parental genomes unification.
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spelling pubmed-89562892022-03-26 Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote Velez-Aguilera, Griselda Ossareh-Nazari, Batool Van Hove, Lucie Joly, Nicolas Pintard, Lionel eLife Cell Biology Previously, we reported that the Polo-like kinase PLK-1 phosphorylates the single Caenorhabditis elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed that this event is required to form a pronuclear envelope scission event that removes membranes on the juxtaposed oocyte and sperm pronuclear envelopes in the zygote, allowing the parental chromosomes to merge in a single nucleus after segregation (Velez-Aguilera et al., 2020). Here, we show that cortical microtubule pulling forces contribute to pronuclear envelopes scission by promoting mitotic spindle elongation, and conversely, nuclear envelopes remodeling facilitates spindle elongation. We also demonstrate that weakening the pronuclear envelopes via PLK-1-mediated lamina depolymerization, is a prerequisite for the astral microtubule pulling forces to trigger pronuclear membranes scission. Finally, we provide evidence that PLK-1 mainly acts via lamina depolymerization in this process. These observations thus indicate that temporal coordination between lamina depolymerization and mitotic spindle elongation facilitates pronuclear envelopes scission and parental genomes unification. eLife Sciences Publications, Ltd 2022-03-08 /pmc/articles/PMC8956289/ /pubmed/35259092 http://dx.doi.org/10.7554/eLife.75382 Text en © 2022, Velez-Aguilera et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Velez-Aguilera, Griselda
Ossareh-Nazari, Batool
Van Hove, Lucie
Joly, Nicolas
Pintard, Lionel
Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote
title Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote
title_full Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote
title_fullStr Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote
title_full_unstemmed Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote
title_short Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote
title_sort cortical microtubule pulling forces contribute to the union of the parental genomes in the caenorhabditis elegans zygote
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956289/
https://www.ncbi.nlm.nih.gov/pubmed/35259092
http://dx.doi.org/10.7554/eLife.75382
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