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Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid

The number of antibiotic-resistant bacterial strains is increasing due to the excessive and inappropriate use of antibiotics, which are therefore becoming ineffective. Here, we report an effective way of enhancing and restoring the antibacterial activity of inactive antibiotics by applying them toge...

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Autores principales: Hochvaldová, Lucie, Panáček, David, Válková, Lucie, Prucek, Robert, Kohlová, Věra, Večeřová, Renata, Kolář, Milan, Kvítek, Libor, Panáček, Aleš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956642/
https://www.ncbi.nlm.nih.gov/pubmed/35338239
http://dx.doi.org/10.1038/s41598-022-09294-7
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author Hochvaldová, Lucie
Panáček, David
Válková, Lucie
Prucek, Robert
Kohlová, Věra
Večeřová, Renata
Kolář, Milan
Kvítek, Libor
Panáček, Aleš
author_facet Hochvaldová, Lucie
Panáček, David
Válková, Lucie
Prucek, Robert
Kohlová, Věra
Večeřová, Renata
Kolář, Milan
Kvítek, Libor
Panáček, Aleš
author_sort Hochvaldová, Lucie
collection PubMed
description The number of antibiotic-resistant bacterial strains is increasing due to the excessive and inappropriate use of antibiotics, which are therefore becoming ineffective. Here, we report an effective way of enhancing and restoring the antibacterial activity of inactive antibiotics by applying them together with a cyanographene/Ag nanohybrid, a nanomaterial that is applied for the first time for restoring the antibacterial activity of antibiotics. The cyanographene/Ag nanohybrid was synthesized by chemical reduction of a precursor material in which silver cations are coordinated on a cyanographene sheet. The antibacterial efficiency of the combined treatment was evaluated by determining fractional inhibitory concentrations (FIC) for antibiotics with different modes of action (gentamicin, ceftazidime, ciprofloxacin, and colistin) against the strains Escherichia coli, Pseudomonas aeruginosa, and Enterobacter kobei with different resistance mechanisms. Synergistic and partial synergistic effects against multiresistant strains were demonstrated for all of these antibiotics except ciprofloxacin, which exhibited an additive effect. The lowest average FICs equal to 0.29 and 0.39 were obtained for colistin against E. kobei and for gentamicin against E. coli, respectively. More importantly, we have experimentally confirmed for the first time, that interaction between the antibiotic's mode of action and the mechanism of bacterial resistance strongly influenced the combined treatment’s efficacy.
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spelling pubmed-89566422022-03-28 Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid Hochvaldová, Lucie Panáček, David Válková, Lucie Prucek, Robert Kohlová, Věra Večeřová, Renata Kolář, Milan Kvítek, Libor Panáček, Aleš Sci Rep Article The number of antibiotic-resistant bacterial strains is increasing due to the excessive and inappropriate use of antibiotics, which are therefore becoming ineffective. Here, we report an effective way of enhancing and restoring the antibacterial activity of inactive antibiotics by applying them together with a cyanographene/Ag nanohybrid, a nanomaterial that is applied for the first time for restoring the antibacterial activity of antibiotics. The cyanographene/Ag nanohybrid was synthesized by chemical reduction of a precursor material in which silver cations are coordinated on a cyanographene sheet. The antibacterial efficiency of the combined treatment was evaluated by determining fractional inhibitory concentrations (FIC) for antibiotics with different modes of action (gentamicin, ceftazidime, ciprofloxacin, and colistin) against the strains Escherichia coli, Pseudomonas aeruginosa, and Enterobacter kobei with different resistance mechanisms. Synergistic and partial synergistic effects against multiresistant strains were demonstrated for all of these antibiotics except ciprofloxacin, which exhibited an additive effect. The lowest average FICs equal to 0.29 and 0.39 were obtained for colistin against E. kobei and for gentamicin against E. coli, respectively. More importantly, we have experimentally confirmed for the first time, that interaction between the antibiotic's mode of action and the mechanism of bacterial resistance strongly influenced the combined treatment’s efficacy. Nature Publishing Group UK 2022-03-25 /pmc/articles/PMC8956642/ /pubmed/35338239 http://dx.doi.org/10.1038/s41598-022-09294-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hochvaldová, Lucie
Panáček, David
Válková, Lucie
Prucek, Robert
Kohlová, Věra
Večeřová, Renata
Kolář, Milan
Kvítek, Libor
Panáček, Aleš
Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid
title Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid
title_full Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid
title_fullStr Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid
title_full_unstemmed Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid
title_short Restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/Ag nanohybrid
title_sort restoration of antibacterial activity of inactive antibiotics via combined treatment with a cyanographene/ag nanohybrid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956642/
https://www.ncbi.nlm.nih.gov/pubmed/35338239
http://dx.doi.org/10.1038/s41598-022-09294-7
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