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MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis
Linkage between microRNAs (miRNAs) and pre-eclampsia (PE) has been documented. Here, we focused on miR-495 in PE and its underlying mechanism in regulation of trophoblast cells. Expression of miR-495, HDAC2, p53 and PUMA was determined in collected placental tissue samples. Loss- and gain-function w...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956677/ https://www.ncbi.nlm.nih.gov/pubmed/35338123 http://dx.doi.org/10.1038/s41420-022-00874-0 |
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author | Zhao, Yi Zhao, Ge Li, Weiwei |
author_facet | Zhao, Yi Zhao, Ge Li, Weiwei |
author_sort | Zhao, Yi |
collection | PubMed |
description | Linkage between microRNAs (miRNAs) and pre-eclampsia (PE) has been documented. Here, we focused on miR-495 in PE and its underlying mechanism in regulation of trophoblast cells. Expression of miR-495, HDAC2, p53 and PUMA was determined in collected placental tissue samples. Loss- and gain-function was performed to determine the roles of miR-495, HDAC2, p53, and PUMA in biological processes of HTR8/SVneo cells and primary trophoblast cells. The relationships among miR-495, HDAC2, and p53 were pinpointed. PE patients presented with higher expression of miR-495, p53, and PUMA in placental tissues, but lower HDAC2. miR-495 negatively targeted HDAC2 expression. HDAC2 suppressed p53 expression via deacetylation. Overexpression of miR-495, p53, or PUMA inhibited biological properties of HTR8/SVneo cells and primary trophoblast cells, while opposite trends were observed in response to oe-HDAC2. In conclusion, miR-495 knockdown can suppress p53/PUMA axis by targeting HDAC2 to enhance biological behaviors of trophoblast cells, which may prevent occurrence of PE. |
format | Online Article Text |
id | pubmed-8956677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89566772022-04-11 MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis Zhao, Yi Zhao, Ge Li, Weiwei Cell Death Discov Article Linkage between microRNAs (miRNAs) and pre-eclampsia (PE) has been documented. Here, we focused on miR-495 in PE and its underlying mechanism in regulation of trophoblast cells. Expression of miR-495, HDAC2, p53 and PUMA was determined in collected placental tissue samples. Loss- and gain-function was performed to determine the roles of miR-495, HDAC2, p53, and PUMA in biological processes of HTR8/SVneo cells and primary trophoblast cells. The relationships among miR-495, HDAC2, and p53 were pinpointed. PE patients presented with higher expression of miR-495, p53, and PUMA in placental tissues, but lower HDAC2. miR-495 negatively targeted HDAC2 expression. HDAC2 suppressed p53 expression via deacetylation. Overexpression of miR-495, p53, or PUMA inhibited biological properties of HTR8/SVneo cells and primary trophoblast cells, while opposite trends were observed in response to oe-HDAC2. In conclusion, miR-495 knockdown can suppress p53/PUMA axis by targeting HDAC2 to enhance biological behaviors of trophoblast cells, which may prevent occurrence of PE. Nature Publishing Group UK 2022-03-25 /pmc/articles/PMC8956677/ /pubmed/35338123 http://dx.doi.org/10.1038/s41420-022-00874-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhao, Yi Zhao, Ge Li, Weiwei MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis |
title | MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis |
title_full | MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis |
title_fullStr | MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis |
title_full_unstemmed | MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis |
title_short | MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis |
title_sort | microrna-495 suppresses pre-eclampsia via activation of p53/puma axis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956677/ https://www.ncbi.nlm.nih.gov/pubmed/35338123 http://dx.doi.org/10.1038/s41420-022-00874-0 |
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