Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts

The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of ge...

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Detalles Bibliográficos
Autores principales: Nilsson, Olof Rickard, Kari, Laszlo, Rosenke, Rebecca, Steele-Mortimer, Olivia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956821/
https://www.ncbi.nlm.nih.gov/pubmed/35345596
http://dx.doi.org/10.1016/j.xpro.2022.101256
Descripción
Sumario:The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial gene expression within the meninges.

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