Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts

The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of ge...

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Autores principales: Nilsson, Olof Rickard, Kari, Laszlo, Rosenke, Rebecca, Steele-Mortimer, Olivia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956821/
https://www.ncbi.nlm.nih.gov/pubmed/35345596
http://dx.doi.org/10.1016/j.xpro.2022.101256
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author Nilsson, Olof Rickard
Kari, Laszlo
Rosenke, Rebecca
Steele-Mortimer, Olivia
author_facet Nilsson, Olof Rickard
Kari, Laszlo
Rosenke, Rebecca
Steele-Mortimer, Olivia
author_sort Nilsson, Olof Rickard
collection PubMed
description The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial gene expression within the meninges.
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spelling pubmed-89568212022-03-27 Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts Nilsson, Olof Rickard Kari, Laszlo Rosenke, Rebecca Steele-Mortimer, Olivia STAR Protoc Protocol The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial gene expression within the meninges. Elsevier 2022-03-22 /pmc/articles/PMC8956821/ /pubmed/35345596 http://dx.doi.org/10.1016/j.xpro.2022.101256 Text en © 2022. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Nilsson, Olof Rickard
Kari, Laszlo
Rosenke, Rebecca
Steele-Mortimer, Olivia
Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
title Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
title_full Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
title_fullStr Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
title_full_unstemmed Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
title_short Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
title_sort protocol for rna fluorescence in situ hybridization in mouse meningeal whole mounts
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956821/
https://www.ncbi.nlm.nih.gov/pubmed/35345596
http://dx.doi.org/10.1016/j.xpro.2022.101256
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