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Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collag...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957015/ https://www.ncbi.nlm.nih.gov/pubmed/35345460 http://dx.doi.org/10.1016/j.isci.2022.104059 |
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author | Al Hosni, Rawiya Bozec, Laurent Roberts, Scott J. Cheema, Umber |
author_facet | Al Hosni, Rawiya Bozec, Laurent Roberts, Scott J. Cheema, Umber |
author_sort | Al Hosni, Rawiya |
collection | PubMed |
description | The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collagen density-specific profile for early and late stage bone cell lineage status. Cell potency was enhanced when AMSCs were cultured within low collagen density environments in hypoxic conditions. A transitional culture containing varied collagen densities in hypoxic conditions directed differential cell fate responses. The early skeletal progenitor identity (PDPN(+)CD146(−)CD73(+)CD164(+)) was rescued in the cells which migrated into low collagen density gels, with cells continuously exposed to the high collagen density gels displaying a transitioned bone-cartilage-stromal phenotype (PDPN(+)CD146(+)CD73(−)CD164(-)). This study uncovers the significant contributions of the physical and physiological cell environment and highlights a chemically independent methodology for reprogramming and isolating skeletal progenitor cells from an adipose-derived cell population. |
format | Online Article Text |
id | pubmed-8957015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89570152022-03-27 Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension Al Hosni, Rawiya Bozec, Laurent Roberts, Scott J. Cheema, Umber iScience Article The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collagen density-specific profile for early and late stage bone cell lineage status. Cell potency was enhanced when AMSCs were cultured within low collagen density environments in hypoxic conditions. A transitional culture containing varied collagen densities in hypoxic conditions directed differential cell fate responses. The early skeletal progenitor identity (PDPN(+)CD146(−)CD73(+)CD164(+)) was rescued in the cells which migrated into low collagen density gels, with cells continuously exposed to the high collagen density gels displaying a transitioned bone-cartilage-stromal phenotype (PDPN(+)CD146(+)CD73(−)CD164(-)). This study uncovers the significant contributions of the physical and physiological cell environment and highlights a chemically independent methodology for reprogramming and isolating skeletal progenitor cells from an adipose-derived cell population. Elsevier 2022-03-11 /pmc/articles/PMC8957015/ /pubmed/35345460 http://dx.doi.org/10.1016/j.isci.2022.104059 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Al Hosni, Rawiya Bozec, Laurent Roberts, Scott J. Cheema, Umber Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
title | Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
title_full | Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
title_fullStr | Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
title_full_unstemmed | Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
title_short | Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
title_sort | reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957015/ https://www.ncbi.nlm.nih.gov/pubmed/35345460 http://dx.doi.org/10.1016/j.isci.2022.104059 |
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