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Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension

The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collag...

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Detalles Bibliográficos
Autores principales: Al Hosni, Rawiya, Bozec, Laurent, Roberts, Scott J., Cheema, Umber
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957015/
https://www.ncbi.nlm.nih.gov/pubmed/35345460
http://dx.doi.org/10.1016/j.isci.2022.104059
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author Al Hosni, Rawiya
Bozec, Laurent
Roberts, Scott J.
Cheema, Umber
author_facet Al Hosni, Rawiya
Bozec, Laurent
Roberts, Scott J.
Cheema, Umber
author_sort Al Hosni, Rawiya
collection PubMed
description The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collagen density-specific profile for early and late stage bone cell lineage status. Cell potency was enhanced when AMSCs were cultured within low collagen density environments in hypoxic conditions. A transitional culture containing varied collagen densities in hypoxic conditions directed differential cell fate responses. The early skeletal progenitor identity (PDPN(+)CD146(−)CD73(+)CD164(+)) was rescued in the cells which migrated into low collagen density gels, with cells continuously exposed to the high collagen density gels displaying a transitioned bone-cartilage-stromal phenotype (PDPN(+)CD146(+)CD73(−)CD164(-)). This study uncovers the significant contributions of the physical and physiological cell environment and highlights a chemically independent methodology for reprogramming and isolating skeletal progenitor cells from an adipose-derived cell population.
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spelling pubmed-89570152022-03-27 Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension Al Hosni, Rawiya Bozec, Laurent Roberts, Scott J. Cheema, Umber iScience Article The biophysical microenvironment of the cell is being increasingly used to control cell signaling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collagen density-specific profile for early and late stage bone cell lineage status. Cell potency was enhanced when AMSCs were cultured within low collagen density environments in hypoxic conditions. A transitional culture containing varied collagen densities in hypoxic conditions directed differential cell fate responses. The early skeletal progenitor identity (PDPN(+)CD146(−)CD73(+)CD164(+)) was rescued in the cells which migrated into low collagen density gels, with cells continuously exposed to the high collagen density gels displaying a transitioned bone-cartilage-stromal phenotype (PDPN(+)CD146(+)CD73(−)CD164(-)). This study uncovers the significant contributions of the physical and physiological cell environment and highlights a chemically independent methodology for reprogramming and isolating skeletal progenitor cells from an adipose-derived cell population. Elsevier 2022-03-11 /pmc/articles/PMC8957015/ /pubmed/35345460 http://dx.doi.org/10.1016/j.isci.2022.104059 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al Hosni, Rawiya
Bozec, Laurent
Roberts, Scott J.
Cheema, Umber
Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
title Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
title_full Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
title_fullStr Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
title_full_unstemmed Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
title_short Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
title_sort reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957015/
https://www.ncbi.nlm.nih.gov/pubmed/35345460
http://dx.doi.org/10.1016/j.isci.2022.104059
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