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Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species
Crocins are high-value soluble pigments that are used as colorants and supplements, their presence in nature is extremely limited and, consequently, the high cost of these metabolites hinders their use by other sectors, such as the pharmaceutical and cosmetic industries. The carotenoid cleavage diox...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957871/ https://www.ncbi.nlm.nih.gov/pubmed/35350302 http://dx.doi.org/10.3389/fpls.2022.861140 |
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author | Ahrazem, Oussama Zhu, Changfu Huang, Xin Rubio-Moraga, Angela Capell, Teresa Christou, Paul Gómez-Gómez, Lourdes |
author_facet | Ahrazem, Oussama Zhu, Changfu Huang, Xin Rubio-Moraga, Angela Capell, Teresa Christou, Paul Gómez-Gómez, Lourdes |
author_sort | Ahrazem, Oussama |
collection | PubMed |
description | Crocins are high-value soluble pigments that are used as colorants and supplements, their presence in nature is extremely limited and, consequently, the high cost of these metabolites hinders their use by other sectors, such as the pharmaceutical and cosmetic industries. The carotenoid cleavage dioxygenase 2L (CsCCD2L) is the key enzyme in the biosynthetic pathway of crocins in Crocus sativus. In this study, CsCCD2L was introduced into Nicotiana tabacum and Nicotiana glauca for the production of crocins. In addition, a chimeric construct containing the Brevundimonas sp. β-carotene hydroxylase (BrCrtZ), the Arabidopsis thaliana ORANGE mutant gene (AtOrMut), and CsCCD2L was also introduced into N. tabacum. Quantitative and qualitative studies on carotenoids and apocarotenoids in the transgenic plants expressing CsCCD2L alone showed higher crocin level accumulation in N. glauca transgenic plants, reaching almost 400 μg/g DW in leaves, while in N. tabacum 36 μg/g DW was obtained. In contrast, N. tabacum plants coexpressing CsCCD2L, BrCrtZ, and AtOrMut accumulated, 3.5-fold compared to N. tabacum plants only expressing CsCCD2L. Crocins with three and four sugar molecules were the main molecular species in both host systems. Our results demonstrate that the production of saffron apocarotenoids is feasible in engineered Nicotiana species and establishes a basis for the development of strategies that may ultimately lead to the commercial exploitation of these valuable pigments for multiple applications. |
format | Online Article Text |
id | pubmed-8957871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89578712022-03-28 Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species Ahrazem, Oussama Zhu, Changfu Huang, Xin Rubio-Moraga, Angela Capell, Teresa Christou, Paul Gómez-Gómez, Lourdes Front Plant Sci Plant Science Crocins are high-value soluble pigments that are used as colorants and supplements, their presence in nature is extremely limited and, consequently, the high cost of these metabolites hinders their use by other sectors, such as the pharmaceutical and cosmetic industries. The carotenoid cleavage dioxygenase 2L (CsCCD2L) is the key enzyme in the biosynthetic pathway of crocins in Crocus sativus. In this study, CsCCD2L was introduced into Nicotiana tabacum and Nicotiana glauca for the production of crocins. In addition, a chimeric construct containing the Brevundimonas sp. β-carotene hydroxylase (BrCrtZ), the Arabidopsis thaliana ORANGE mutant gene (AtOrMut), and CsCCD2L was also introduced into N. tabacum. Quantitative and qualitative studies on carotenoids and apocarotenoids in the transgenic plants expressing CsCCD2L alone showed higher crocin level accumulation in N. glauca transgenic plants, reaching almost 400 μg/g DW in leaves, while in N. tabacum 36 μg/g DW was obtained. In contrast, N. tabacum plants coexpressing CsCCD2L, BrCrtZ, and AtOrMut accumulated, 3.5-fold compared to N. tabacum plants only expressing CsCCD2L. Crocins with three and four sugar molecules were the main molecular species in both host systems. Our results demonstrate that the production of saffron apocarotenoids is feasible in engineered Nicotiana species and establishes a basis for the development of strategies that may ultimately lead to the commercial exploitation of these valuable pigments for multiple applications. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8957871/ /pubmed/35350302 http://dx.doi.org/10.3389/fpls.2022.861140 Text en Copyright © 2022 Ahrazem, Zhu, Huang, Rubio-Moraga, Capell, Christou and Gómez-Gómez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Ahrazem, Oussama Zhu, Changfu Huang, Xin Rubio-Moraga, Angela Capell, Teresa Christou, Paul Gómez-Gómez, Lourdes Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species |
title | Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species |
title_full | Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species |
title_fullStr | Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species |
title_full_unstemmed | Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species |
title_short | Metabolic Engineering of Crocin Biosynthesis in Nicotiana Species |
title_sort | metabolic engineering of crocin biosynthesis in nicotiana species |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957871/ https://www.ncbi.nlm.nih.gov/pubmed/35350302 http://dx.doi.org/10.3389/fpls.2022.861140 |
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