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Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism

Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism p...

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Autores principales: Song, Jia, Wang, Jun, Wang, Xinyu, Zhao, Hang, Hu, Tao, Feng, Zhiwei, Lei, Zhi, Li, Weizhao, Zheng, Yu, Wang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957916/
https://www.ncbi.nlm.nih.gov/pubmed/35350179
http://dx.doi.org/10.3389/fbioe.2022.815614
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author Song, Jia
Wang, Jun
Wang, Xinyu
Zhao, Hang
Hu, Tao
Feng, Zhiwei
Lei, Zhi
Li, Weizhao
Zheng, Yu
Wang, Min
author_facet Song, Jia
Wang, Jun
Wang, Xinyu
Zhao, Hang
Hu, Tao
Feng, Zhiwei
Lei, Zhi
Li, Weizhao
Zheng, Yu
Wang, Min
author_sort Song, Jia
collection PubMed
description Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism pathway, including glycolysis pathway, TCA cycle, ethanol oxidation, pentose phosphate pathway, and ATP production, was constructed by transcriptome analysis. The effects of fermentation conditions, including dissolved oxygen, initial acetic acid concentration, and total concentration, on acetic acid fermentation and energy metabolism of A. pasteurianus were analyzed by using the RT-PCR method. The results showed the high energy charge inhibited glucose catabolism, and associated with the high ethanol oxidation rate. Consequently, a virtuous circle of increased ethanol oxidation, increased energy generation, and acetic acid tolerance was important for improving acetic acid fermentation.
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spelling pubmed-89579162022-03-28 Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism Song, Jia Wang, Jun Wang, Xinyu Zhao, Hang Hu, Tao Feng, Zhiwei Lei, Zhi Li, Weizhao Zheng, Yu Wang, Min Front Bioeng Biotechnol Bioengineering and Biotechnology Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism pathway, including glycolysis pathway, TCA cycle, ethanol oxidation, pentose phosphate pathway, and ATP production, was constructed by transcriptome analysis. The effects of fermentation conditions, including dissolved oxygen, initial acetic acid concentration, and total concentration, on acetic acid fermentation and energy metabolism of A. pasteurianus were analyzed by using the RT-PCR method. The results showed the high energy charge inhibited glucose catabolism, and associated with the high ethanol oxidation rate. Consequently, a virtuous circle of increased ethanol oxidation, increased energy generation, and acetic acid tolerance was important for improving acetic acid fermentation. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8957916/ /pubmed/35350179 http://dx.doi.org/10.3389/fbioe.2022.815614 Text en Copyright © 2022 Song, Wang, Wang, Zhao, Hu, Feng, Lei, Li, Zheng and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Song, Jia
Wang, Jun
Wang, Xinyu
Zhao, Hang
Hu, Tao
Feng, Zhiwei
Lei, Zhi
Li, Weizhao
Zheng, Yu
Wang, Min
Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
title Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
title_full Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
title_fullStr Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
title_full_unstemmed Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
title_short Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
title_sort improving the acetic acid fermentation of acetobacter pasteurianus by enhancing the energy metabolism
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957916/
https://www.ncbi.nlm.nih.gov/pubmed/35350179
http://dx.doi.org/10.3389/fbioe.2022.815614
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