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Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism
Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism p...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957916/ https://www.ncbi.nlm.nih.gov/pubmed/35350179 http://dx.doi.org/10.3389/fbioe.2022.815614 |
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author | Song, Jia Wang, Jun Wang, Xinyu Zhao, Hang Hu, Tao Feng, Zhiwei Lei, Zhi Li, Weizhao Zheng, Yu Wang, Min |
author_facet | Song, Jia Wang, Jun Wang, Xinyu Zhao, Hang Hu, Tao Feng, Zhiwei Lei, Zhi Li, Weizhao Zheng, Yu Wang, Min |
author_sort | Song, Jia |
collection | PubMed |
description | Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism pathway, including glycolysis pathway, TCA cycle, ethanol oxidation, pentose phosphate pathway, and ATP production, was constructed by transcriptome analysis. The effects of fermentation conditions, including dissolved oxygen, initial acetic acid concentration, and total concentration, on acetic acid fermentation and energy metabolism of A. pasteurianus were analyzed by using the RT-PCR method. The results showed the high energy charge inhibited glucose catabolism, and associated with the high ethanol oxidation rate. Consequently, a virtuous circle of increased ethanol oxidation, increased energy generation, and acetic acid tolerance was important for improving acetic acid fermentation. |
format | Online Article Text |
id | pubmed-8957916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89579162022-03-28 Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism Song, Jia Wang, Jun Wang, Xinyu Zhao, Hang Hu, Tao Feng, Zhiwei Lei, Zhi Li, Weizhao Zheng, Yu Wang, Min Front Bioeng Biotechnol Bioengineering and Biotechnology Energy metabolism is important for cell growth and tolerance against environment stress. In acetic acid fermentation by Acetobacter pasteurianus, the correlation coefficients of acid production rate with energy charge and ATP content were 0.9981 and 0.9826, respectively. The main energy metabolism pathway, including glycolysis pathway, TCA cycle, ethanol oxidation, pentose phosphate pathway, and ATP production, was constructed by transcriptome analysis. The effects of fermentation conditions, including dissolved oxygen, initial acetic acid concentration, and total concentration, on acetic acid fermentation and energy metabolism of A. pasteurianus were analyzed by using the RT-PCR method. The results showed the high energy charge inhibited glucose catabolism, and associated with the high ethanol oxidation rate. Consequently, a virtuous circle of increased ethanol oxidation, increased energy generation, and acetic acid tolerance was important for improving acetic acid fermentation. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8957916/ /pubmed/35350179 http://dx.doi.org/10.3389/fbioe.2022.815614 Text en Copyright © 2022 Song, Wang, Wang, Zhao, Hu, Feng, Lei, Li, Zheng and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Song, Jia Wang, Jun Wang, Xinyu Zhao, Hang Hu, Tao Feng, Zhiwei Lei, Zhi Li, Weizhao Zheng, Yu Wang, Min Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism |
title | Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism |
title_full | Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism |
title_fullStr | Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism |
title_full_unstemmed | Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism |
title_short | Improving the Acetic Acid Fermentation of Acetobacter pasteurianus by Enhancing the Energy Metabolism |
title_sort | improving the acetic acid fermentation of acetobacter pasteurianus by enhancing the energy metabolism |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957916/ https://www.ncbi.nlm.nih.gov/pubmed/35350179 http://dx.doi.org/10.3389/fbioe.2022.815614 |
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