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Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography

Synchrotron radiation offers unique properties of coherence, utilized in phase-contrast imaging, and high flux as well as a wide energy spectrum which allow the selection of very narrow energy bands of radiation, used in K-edge subtraction imaging (KES) imaging. These properties extend X-ray compute...

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Autores principales: Bayat, Sam, Fardin, Luca, Cercos-Pita, José Luis, Perchiazzi, Gaetano, Bravin, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957951/
https://www.ncbi.nlm.nih.gov/pubmed/35350681
http://dx.doi.org/10.3389/fphys.2022.825433
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author Bayat, Sam
Fardin, Luca
Cercos-Pita, José Luis
Perchiazzi, Gaetano
Bravin, Alberto
author_facet Bayat, Sam
Fardin, Luca
Cercos-Pita, José Luis
Perchiazzi, Gaetano
Bravin, Alberto
author_sort Bayat, Sam
collection PubMed
description Synchrotron radiation offers unique properties of coherence, utilized in phase-contrast imaging, and high flux as well as a wide energy spectrum which allow the selection of very narrow energy bands of radiation, used in K-edge subtraction imaging (KES) imaging. These properties extend X-ray computed tomography (CT) capabilities to quantitatively assess lung morphology, and to map regional lung ventilation, perfusion, inflammation, aerosol particle distribution and biomechanical properties, with microscopic spatial resolution. Four-dimensional imaging, allows the investigation of the dynamics of regional lung functional parameters simultaneously with structural deformation of the lung as a function of time. These techniques have proven to be very useful for revealing the regional differences in both lung structure and function which is crucial for better understanding of disease mechanisms as well as for evaluating treatment in small animal models of lung diseases. Here, synchrotron radiation imaging methods are described and examples of their application to the study of disease mechanisms in preclinical animal models are presented.
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spelling pubmed-89579512022-03-28 Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography Bayat, Sam Fardin, Luca Cercos-Pita, José Luis Perchiazzi, Gaetano Bravin, Alberto Front Physiol Physiology Synchrotron radiation offers unique properties of coherence, utilized in phase-contrast imaging, and high flux as well as a wide energy spectrum which allow the selection of very narrow energy bands of radiation, used in K-edge subtraction imaging (KES) imaging. These properties extend X-ray computed tomography (CT) capabilities to quantitatively assess lung morphology, and to map regional lung ventilation, perfusion, inflammation, aerosol particle distribution and biomechanical properties, with microscopic spatial resolution. Four-dimensional imaging, allows the investigation of the dynamics of regional lung functional parameters simultaneously with structural deformation of the lung as a function of time. These techniques have proven to be very useful for revealing the regional differences in both lung structure and function which is crucial for better understanding of disease mechanisms as well as for evaluating treatment in small animal models of lung diseases. Here, synchrotron radiation imaging methods are described and examples of their application to the study of disease mechanisms in preclinical animal models are presented. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8957951/ /pubmed/35350681 http://dx.doi.org/10.3389/fphys.2022.825433 Text en Copyright © 2022 Bayat, Fardin, Cercos-Pita, Perchiazzi and Bravin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Bayat, Sam
Fardin, Luca
Cercos-Pita, José Luis
Perchiazzi, Gaetano
Bravin, Alberto
Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography
title Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography
title_full Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography
title_fullStr Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography
title_full_unstemmed Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography
title_short Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography
title_sort imaging regional lung structure and function in small animals using synchrotron radiation phase-contrast and k-edge subtraction computed tomography
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957951/
https://www.ncbi.nlm.nih.gov/pubmed/35350681
http://dx.doi.org/10.3389/fphys.2022.825433
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