Cargando…
Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environ...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957974/ https://www.ncbi.nlm.nih.gov/pubmed/35350186 http://dx.doi.org/10.3389/fbioe.2022.843887 |
_version_ | 1784676849244176384 |
---|---|
author | Liu, Xiutao Zhao, Guang Sun, Shengjie Fan, Chuanle Feng, Xinjun Xiong, Peng |
author_facet | Liu, Xiutao Zhao, Guang Sun, Shengjie Fan, Chuanle Feng, Xinjun Xiong, Peng |
author_sort | Liu, Xiutao |
collection | PubMed |
description | Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD(+) ratios, and optimizing CO(2) supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed. |
format | Online Article Text |
id | pubmed-8957974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89579742022-03-28 Biosynthetic Pathway and Metabolic Engineering of Succinic Acid Liu, Xiutao Zhao, Guang Sun, Shengjie Fan, Chuanle Feng, Xinjun Xiong, Peng Front Bioeng Biotechnol Bioengineering and Biotechnology Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD(+) ratios, and optimizing CO(2) supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8957974/ /pubmed/35350186 http://dx.doi.org/10.3389/fbioe.2022.843887 Text en Copyright © 2022 Liu, Zhao, Sun, Fan, Feng and Xiong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Liu, Xiutao Zhao, Guang Sun, Shengjie Fan, Chuanle Feng, Xinjun Xiong, Peng Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
title | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
title_full | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
title_fullStr | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
title_full_unstemmed | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
title_short | Biosynthetic Pathway and Metabolic Engineering of Succinic Acid |
title_sort | biosynthetic pathway and metabolic engineering of succinic acid |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957974/ https://www.ncbi.nlm.nih.gov/pubmed/35350186 http://dx.doi.org/10.3389/fbioe.2022.843887 |
work_keys_str_mv | AT liuxiutao biosyntheticpathwayandmetabolicengineeringofsuccinicacid AT zhaoguang biosyntheticpathwayandmetabolicengineeringofsuccinicacid AT sunshengjie biosyntheticpathwayandmetabolicengineeringofsuccinicacid AT fanchuanle biosyntheticpathwayandmetabolicengineeringofsuccinicacid AT fengxinjun biosyntheticpathwayandmetabolicengineeringofsuccinicacid AT xiongpeng biosyntheticpathwayandmetabolicengineeringofsuccinicacid |