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Biosynthetic Pathway and Metabolic Engineering of Succinic Acid

Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environ...

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Autores principales: Liu, Xiutao, Zhao, Guang, Sun, Shengjie, Fan, Chuanle, Feng, Xinjun, Xiong, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957974/
https://www.ncbi.nlm.nih.gov/pubmed/35350186
http://dx.doi.org/10.3389/fbioe.2022.843887
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author Liu, Xiutao
Zhao, Guang
Sun, Shengjie
Fan, Chuanle
Feng, Xinjun
Xiong, Peng
author_facet Liu, Xiutao
Zhao, Guang
Sun, Shengjie
Fan, Chuanle
Feng, Xinjun
Xiong, Peng
author_sort Liu, Xiutao
collection PubMed
description Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD(+) ratios, and optimizing CO(2) supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed.
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spelling pubmed-89579742022-03-28 Biosynthetic Pathway and Metabolic Engineering of Succinic Acid Liu, Xiutao Zhao, Guang Sun, Shengjie Fan, Chuanle Feng, Xinjun Xiong, Peng Front Bioeng Biotechnol Bioengineering and Biotechnology Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD(+) ratios, and optimizing CO(2) supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8957974/ /pubmed/35350186 http://dx.doi.org/10.3389/fbioe.2022.843887 Text en Copyright © 2022 Liu, Zhao, Sun, Fan, Feng and Xiong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Xiutao
Zhao, Guang
Sun, Shengjie
Fan, Chuanle
Feng, Xinjun
Xiong, Peng
Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
title Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
title_full Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
title_fullStr Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
title_full_unstemmed Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
title_short Biosynthetic Pathway and Metabolic Engineering of Succinic Acid
title_sort biosynthetic pathway and metabolic engineering of succinic acid
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957974/
https://www.ncbi.nlm.nih.gov/pubmed/35350186
http://dx.doi.org/10.3389/fbioe.2022.843887
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