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The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear

The rabbit supraspinatus is a useful translational model for rotator cuff (RC) repair because it recapitulates muscle atrophy and fat accumulation observed in humans after a chronic tear (the “first hit”). However, a timeline of RC tissue response after repair, especially with regard to recent evide...

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Autores principales: Wu, Isabella T., Gibbons, Michael C., Esparza, Mary C., Vasquez-Bolanos, Laura S., Hyman, Sydnee A., Dorn, Shanelle N., Singh, Anshuman, Lane, John G., Fithian, Donald C., Ruoss, Severin, Ward, Samuel R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958027/
https://www.ncbi.nlm.nih.gov/pubmed/35350696
http://dx.doi.org/10.3389/fphys.2022.801829
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author Wu, Isabella T.
Gibbons, Michael C.
Esparza, Mary C.
Vasquez-Bolanos, Laura S.
Hyman, Sydnee A.
Dorn, Shanelle N.
Singh, Anshuman
Lane, John G.
Fithian, Donald C.
Ruoss, Severin
Ward, Samuel R.
author_facet Wu, Isabella T.
Gibbons, Michael C.
Esparza, Mary C.
Vasquez-Bolanos, Laura S.
Hyman, Sydnee A.
Dorn, Shanelle N.
Singh, Anshuman
Lane, John G.
Fithian, Donald C.
Ruoss, Severin
Ward, Samuel R.
author_sort Wu, Isabella T.
collection PubMed
description The rabbit supraspinatus is a useful translational model for rotator cuff (RC) repair because it recapitulates muscle atrophy and fat accumulation observed in humans after a chronic tear (the “first hit”). However, a timeline of RC tissue response after repair, especially with regard to recent evidence of muscle degeneration and lack of regeneration, is currently unavailable. Thus, the purpose of this study was to characterize the progression of muscle and fat changes over time after the repair of a chronic RC tear in the rabbit model. Two rounds of experiments were conducted in 2017–2018 and 2019–2020 with N = 18 and 16 skeletally mature New Zealand White rabbits, respectively. Animals underwent left supraspinatus tenotomy with repair 8 weeks later. The unoperated right shoulder served as control. The rabbits were sacrificed at 1-, 2-, 4-, and 8-weeks post-repair for histological and biochemical analysis. Atrophy, measured by fiber cross-sectional area and muscle mass, was greatest around 2 weeks after repair. Active muscle degeneration peaked at the same time, involving 8% of slide areas. There was no significant regeneration at any timepoint. Fat accumulation and fibrosis were significantly increased across all time points compared to contralateral. Statement of Clinical Significance: These results demonstrate model reproducibility and a “second hit” phenomenon of repair-induced muscle atrophy and degeneration which partially recovers after a short time, while increased fat and fibrosis persist.
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spelling pubmed-89580272022-03-28 The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear Wu, Isabella T. Gibbons, Michael C. Esparza, Mary C. Vasquez-Bolanos, Laura S. Hyman, Sydnee A. Dorn, Shanelle N. Singh, Anshuman Lane, John G. Fithian, Donald C. Ruoss, Severin Ward, Samuel R. Front Physiol Physiology The rabbit supraspinatus is a useful translational model for rotator cuff (RC) repair because it recapitulates muscle atrophy and fat accumulation observed in humans after a chronic tear (the “first hit”). However, a timeline of RC tissue response after repair, especially with regard to recent evidence of muscle degeneration and lack of regeneration, is currently unavailable. Thus, the purpose of this study was to characterize the progression of muscle and fat changes over time after the repair of a chronic RC tear in the rabbit model. Two rounds of experiments were conducted in 2017–2018 and 2019–2020 with N = 18 and 16 skeletally mature New Zealand White rabbits, respectively. Animals underwent left supraspinatus tenotomy with repair 8 weeks later. The unoperated right shoulder served as control. The rabbits were sacrificed at 1-, 2-, 4-, and 8-weeks post-repair for histological and biochemical analysis. Atrophy, measured by fiber cross-sectional area and muscle mass, was greatest around 2 weeks after repair. Active muscle degeneration peaked at the same time, involving 8% of slide areas. There was no significant regeneration at any timepoint. Fat accumulation and fibrosis were significantly increased across all time points compared to contralateral. Statement of Clinical Significance: These results demonstrate model reproducibility and a “second hit” phenomenon of repair-induced muscle atrophy and degeneration which partially recovers after a short time, while increased fat and fibrosis persist. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8958027/ /pubmed/35350696 http://dx.doi.org/10.3389/fphys.2022.801829 Text en Copyright © 2022 Wu, Gibbons, Esparza, Vasquez-Bolanos, Hyman, Dorn, Singh, Lane, Fithian, Ruoss and Ward. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Wu, Isabella T.
Gibbons, Michael C.
Esparza, Mary C.
Vasquez-Bolanos, Laura S.
Hyman, Sydnee A.
Dorn, Shanelle N.
Singh, Anshuman
Lane, John G.
Fithian, Donald C.
Ruoss, Severin
Ward, Samuel R.
The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear
title The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear
title_full The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear
title_fullStr The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear
title_full_unstemmed The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear
title_short The “Second Hit” of Repair in a Rabbit Model of Chronic Rotator Cuff Tear
title_sort “second hit” of repair in a rabbit model of chronic rotator cuff tear
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958027/
https://www.ncbi.nlm.nih.gov/pubmed/35350696
http://dx.doi.org/10.3389/fphys.2022.801829
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