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Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy

Objective: To examine early histologic changes in the aorta exposed to bicuspid flow. Material and Methods: A porcine bicuspid aortopathy model was developed by suturing aortic cusps. Of nine pigs, eight underwent sham surgery (n=3) or bicuspidalization (n=5); one was used as an intact control. Wall...

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Autores principales: Kimura, Naoyuki, Itagaki, Ryo, Nakamura, Masanori, Tofrizal, Alimuddin, Yatabe, Megumi, Yoshizaki, Takamichi, Kokubo, Ryo, Hishikawa, Shuji, Kunita, Satoshi, Adachi, Hideo, Misawa, Yoshio, Yashiro, Takashi, Kawahito, Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese College of Angiology / The Japanese Society for Vascular Surgery / Japanese Society of Phlebology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958402/
https://www.ncbi.nlm.nih.gov/pubmed/35432643
http://dx.doi.org/10.3400/avd.oa.21-00116
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author Kimura, Naoyuki
Itagaki, Ryo
Nakamura, Masanori
Tofrizal, Alimuddin
Yatabe, Megumi
Yoshizaki, Takamichi
Kokubo, Ryo
Hishikawa, Shuji
Kunita, Satoshi
Adachi, Hideo
Misawa, Yoshio
Yashiro, Takashi
Kawahito, Koji
author_facet Kimura, Naoyuki
Itagaki, Ryo
Nakamura, Masanori
Tofrizal, Alimuddin
Yatabe, Megumi
Yoshizaki, Takamichi
Kokubo, Ryo
Hishikawa, Shuji
Kunita, Satoshi
Adachi, Hideo
Misawa, Yoshio
Yashiro, Takashi
Kawahito, Koji
author_sort Kimura, Naoyuki
collection PubMed
description Objective: To examine early histologic changes in the aorta exposed to bicuspid flow. Material and Methods: A porcine bicuspid aortopathy model was developed by suturing aortic cusps. Of nine pigs, eight underwent sham surgery (n=3) or bicuspidalization (n=5); one was used as an intact control. Wall shear stress (WSS) was assessed by computational fluid dynamics (CFD). Animals were exposed to normal or bicuspid flow for 48 h and were then euthanized for histologic examinations. Results: No animal died intraoperatively. One animal subjected to bicuspidalization died of respiratory failure during postoperative imaging studies. Echocardiography showed the aortic valve area decreased from 2.52±1.15 to 1.21±0.48 cm(2) after bicuspidalization, CFD revealed increased maximum WSS (10.0±5.2 vs. 54.0±25.7 Pa; P=0.036) and percentage area of increased WSS (>5 Pa) in the ascending aorta (30.3%±24.1% vs. 81.3%±13.4%; P=0.015) after bicuspidalization. Hematoxylin–eosin staining and transmission electron microscopy showed subintimal edema and detached or degenerated endothelial cells following both sham surgery and bicuspidalization, regardless of WSS distribution. Conclusion: A bicuspid aortic valve appears to increase aortic WSS. The endothelial damage observed might have been related to non-pulsatile flow (cardiopulmonary bypass). Chronic experiments are needed to clarify the relationship between hemodynamic stress and development of bicuspid aortopathy.
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spelling pubmed-89584022022-04-14 Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy Kimura, Naoyuki Itagaki, Ryo Nakamura, Masanori Tofrizal, Alimuddin Yatabe, Megumi Yoshizaki, Takamichi Kokubo, Ryo Hishikawa, Shuji Kunita, Satoshi Adachi, Hideo Misawa, Yoshio Yashiro, Takashi Kawahito, Koji Ann Vasc Dis Original Article Objective: To examine early histologic changes in the aorta exposed to bicuspid flow. Material and Methods: A porcine bicuspid aortopathy model was developed by suturing aortic cusps. Of nine pigs, eight underwent sham surgery (n=3) or bicuspidalization (n=5); one was used as an intact control. Wall shear stress (WSS) was assessed by computational fluid dynamics (CFD). Animals were exposed to normal or bicuspid flow for 48 h and were then euthanized for histologic examinations. Results: No animal died intraoperatively. One animal subjected to bicuspidalization died of respiratory failure during postoperative imaging studies. Echocardiography showed the aortic valve area decreased from 2.52±1.15 to 1.21±0.48 cm(2) after bicuspidalization, CFD revealed increased maximum WSS (10.0±5.2 vs. 54.0±25.7 Pa; P=0.036) and percentage area of increased WSS (>5 Pa) in the ascending aorta (30.3%±24.1% vs. 81.3%±13.4%; P=0.015) after bicuspidalization. Hematoxylin–eosin staining and transmission electron microscopy showed subintimal edema and detached or degenerated endothelial cells following both sham surgery and bicuspidalization, regardless of WSS distribution. Conclusion: A bicuspid aortic valve appears to increase aortic WSS. The endothelial damage observed might have been related to non-pulsatile flow (cardiopulmonary bypass). Chronic experiments are needed to clarify the relationship between hemodynamic stress and development of bicuspid aortopathy. Japanese College of Angiology / The Japanese Society for Vascular Surgery / Japanese Society of Phlebology 2022-03-25 /pmc/articles/PMC8958402/ /pubmed/35432643 http://dx.doi.org/10.3400/avd.oa.21-00116 Text en © 2022 The Editorial Committee of Annals of Vascular Diseases. https://creativecommons.org/licenses/by/2.5/This article is distributed under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the credit of the original work, a link to the license, and indication of any change are properly given, and the original work is not used for commercial purposes. Remixed or transformed contributions must be distributed under the same license as the original.
spellingShingle Original Article
Kimura, Naoyuki
Itagaki, Ryo
Nakamura, Masanori
Tofrizal, Alimuddin
Yatabe, Megumi
Yoshizaki, Takamichi
Kokubo, Ryo
Hishikawa, Shuji
Kunita, Satoshi
Adachi, Hideo
Misawa, Yoshio
Yashiro, Takashi
Kawahito, Koji
Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy
title Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy
title_full Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy
title_fullStr Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy
title_full_unstemmed Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy
title_short Bicuspidalization of the Native Tricuspid Aortic Valve: A Porcine in Vivo Model of Bicuspid Aortopathy
title_sort bicuspidalization of the native tricuspid aortic valve: a porcine in vivo model of bicuspid aortopathy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958402/
https://www.ncbi.nlm.nih.gov/pubmed/35432643
http://dx.doi.org/10.3400/avd.oa.21-00116
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