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High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms

Rotavirus, one of the main pathogens causing morbidity and mortality in neonatal dairy calves worldwide, is responsible for 30–44% of cattle deaths. It is considered to be the most common etiologic agent of diarrhea in neonatal dairy calves and children, the dominant type being group A. Two hundred...

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Autores principales: Alotaibi, Mohammad A., Al-Amad, S., Chenari Bouket, Ali, Al-Aqeel, H., Haider, E., Hijji, A. Bin, Belbahri, Lassaad, Alenezi, Faizah N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959109/
https://www.ncbi.nlm.nih.gov/pubmed/35356787
http://dx.doi.org/10.3389/fvets.2022.745934
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author Alotaibi, Mohammad A.
Al-Amad, S.
Chenari Bouket, Ali
Al-Aqeel, H.
Haider, E.
Hijji, A. Bin
Belbahri, Lassaad
Alenezi, Faizah N.
author_facet Alotaibi, Mohammad A.
Al-Amad, S.
Chenari Bouket, Ali
Al-Aqeel, H.
Haider, E.
Hijji, A. Bin
Belbahri, Lassaad
Alenezi, Faizah N.
author_sort Alotaibi, Mohammad A.
collection PubMed
description Rotavirus, one of the main pathogens causing morbidity and mortality in neonatal dairy calves worldwide, is responsible for 30–44% of cattle deaths. It is considered to be the most common etiologic agent of diarrhea in neonatal dairy calves and children, the dominant type being group A. Two hundred seventy animals from 27 farms from 2 regions of Kuwait were tested for the presence of Rotavirus serogroup A (RVA) using latex agglutination test (LAT) and reverse transcription–polymerase chain (RT-PCR) testing. RVA non-structural proteins NSP1-2, NSP4-5 and capsid protein genes VP1-7 were characterized by next generation sequencing. LAT was positive in 15.56% of the animals, and RT-PCR in 28.89%. Using RT-PCR as a reference method, LAT was 100% specific but only 83.33% sensitive. ANOVA analysis showed correlation only with the location of the farms but no significant correlation with the age and sex of the animals. Although there was a tendency of clustering of RVA positive animals, it did not reach statistical significance (p = 0.035 for LAT). The phylogenetic analysis showed that Kuwaiti isolates of group A rotavirus clustered with human rotaviruses. Taken together, it seems that rotavirus was present in most of the dairy farms in Kuwait. The high occurrence of the virus in calves in Kuwaiti dairy farms and the close phylogenetic affinity with human isolates warrants urgent action to minimize and control its spread between calves in farms.
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spelling pubmed-89591092022-03-29 High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms Alotaibi, Mohammad A. Al-Amad, S. Chenari Bouket, Ali Al-Aqeel, H. Haider, E. Hijji, A. Bin Belbahri, Lassaad Alenezi, Faizah N. Front Vet Sci Veterinary Science Rotavirus, one of the main pathogens causing morbidity and mortality in neonatal dairy calves worldwide, is responsible for 30–44% of cattle deaths. It is considered to be the most common etiologic agent of diarrhea in neonatal dairy calves and children, the dominant type being group A. Two hundred seventy animals from 27 farms from 2 regions of Kuwait were tested for the presence of Rotavirus serogroup A (RVA) using latex agglutination test (LAT) and reverse transcription–polymerase chain (RT-PCR) testing. RVA non-structural proteins NSP1-2, NSP4-5 and capsid protein genes VP1-7 were characterized by next generation sequencing. LAT was positive in 15.56% of the animals, and RT-PCR in 28.89%. Using RT-PCR as a reference method, LAT was 100% specific but only 83.33% sensitive. ANOVA analysis showed correlation only with the location of the farms but no significant correlation with the age and sex of the animals. Although there was a tendency of clustering of RVA positive animals, it did not reach statistical significance (p = 0.035 for LAT). The phylogenetic analysis showed that Kuwaiti isolates of group A rotavirus clustered with human rotaviruses. Taken together, it seems that rotavirus was present in most of the dairy farms in Kuwait. The high occurrence of the virus in calves in Kuwaiti dairy farms and the close phylogenetic affinity with human isolates warrants urgent action to minimize and control its spread between calves in farms. Frontiers Media S.A. 2022-03-08 /pmc/articles/PMC8959109/ /pubmed/35356787 http://dx.doi.org/10.3389/fvets.2022.745934 Text en Copyright © 2022 Alotaibi, Al-Amad, Chenari Bouket, Al-Aqeel, Haider, Hijji, Belbahri and Alenezi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Alotaibi, Mohammad A.
Al-Amad, S.
Chenari Bouket, Ali
Al-Aqeel, H.
Haider, E.
Hijji, A. Bin
Belbahri, Lassaad
Alenezi, Faizah N.
High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms
title High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms
title_full High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms
title_fullStr High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms
title_full_unstemmed High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms
title_short High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms
title_sort high occurrence among calves and close phylogenetic relationships with human viruses warrants close surveillance of rotaviruses in kuwaiti dairy farms
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959109/
https://www.ncbi.nlm.nih.gov/pubmed/35356787
http://dx.doi.org/10.3389/fvets.2022.745934
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